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The proportion of carboxylated to total or intact osteocalcin in serum discriminates warfarin-treated patients from control subjects

Obrant, Karl LU ; Kakonen, S M; Astermark, Jan LU ; Lilja, Hans LU ; Lovgren, T; Åkesson, Kristina LU and Pettersson, K (1999) In Journal of Bone and Mineral Research 14(4). p.555-560
Abstract
We assessed the serum concentration of gamma-carboxylated osteocalcin (OC), total OC, and full-length OC in a clinical setting of 37 patients on continuous warfarin treatment (international normalized ratio 2.0-3.8). A comparison was done with the results from 30 untreated age-matched controls. Four monoclonal antibodies, previously generated and characterized as to their ability to recognize different human OC forms and fragments, were used in three two-site immunofluorometric assays. The warfarin-treated patients had significantly lower levels of carboxylated OC 4.9 +/- 3.8 (+/- 1 SD) ng/ml compared with the controls 13.1 +/- 9.7 (p < 0.0001). There was no difference in the levels of total OC or full-length OC between the two groups... (More)
We assessed the serum concentration of gamma-carboxylated osteocalcin (OC), total OC, and full-length OC in a clinical setting of 37 patients on continuous warfarin treatment (international normalized ratio 2.0-3.8). A comparison was done with the results from 30 untreated age-matched controls. Four monoclonal antibodies, previously generated and characterized as to their ability to recognize different human OC forms and fragments, were used in three two-site immunofluorometric assays. The warfarin-treated patients had significantly lower levels of carboxylated OC 4.9 +/- 3.8 (+/- 1 SD) ng/ml compared with the controls 13.1 +/- 9.7 (p < 0.0001). There was no difference in the levels of total OC or full-length OC between the two groups of patients. A strong correlation was found between the serum concentration of carboxylated OC and total OC, both for the warfarin-treated patients (r = 0.98) and for the controls (r = 0.99). There was a distinct cut-off level at 0.80, in the quotient carboxylated OC/total OC, at which all warfarin-treated patients fell below and all controls above this level. Hence, the concentration or ratio of serum gamma-carboxylated OC in clinical settings such as warfarin-treated patients could be measured using two-site immunoassays. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bone and Mineral Research
volume
14
issue
4
pages
555 - 560
publisher
AMBMR
external identifiers
  • pmid:10234576
  • scopus:0344628892
ISSN
1523-4681
DOI
10.1359/jbmr.1999.14.4.555
language
English
LU publication?
yes
id
140625d3-f0be-4f00-9533-792f7807d55a (old id 1114957)
date added to LUP
2008-07-07 11:35:28
date last changed
2017-01-01 04:45:17
@article{140625d3-f0be-4f00-9533-792f7807d55a,
  abstract     = {We assessed the serum concentration of gamma-carboxylated osteocalcin (OC), total OC, and full-length OC in a clinical setting of 37 patients on continuous warfarin treatment (international normalized ratio 2.0-3.8). A comparison was done with the results from 30 untreated age-matched controls. Four monoclonal antibodies, previously generated and characterized as to their ability to recognize different human OC forms and fragments, were used in three two-site immunofluorometric assays. The warfarin-treated patients had significantly lower levels of carboxylated OC 4.9 +/- 3.8 (+/- 1 SD) ng/ml compared with the controls 13.1 +/- 9.7 (p &lt; 0.0001). There was no difference in the levels of total OC or full-length OC between the two groups of patients. A strong correlation was found between the serum concentration of carboxylated OC and total OC, both for the warfarin-treated patients (r = 0.98) and for the controls (r = 0.99). There was a distinct cut-off level at 0.80, in the quotient carboxylated OC/total OC, at which all warfarin-treated patients fell below and all controls above this level. Hence, the concentration or ratio of serum gamma-carboxylated OC in clinical settings such as warfarin-treated patients could be measured using two-site immunoassays.},
  author       = {Obrant, Karl and Kakonen, S M and Astermark, Jan and Lilja, Hans and Lovgren, T and Åkesson, Kristina and Pettersson, K},
  issn         = {1523-4681},
  language     = {eng},
  number       = {4},
  pages        = {555--560},
  publisher    = {AMBMR},
  series       = {Journal of Bone and Mineral Research},
  title        = {The proportion of carboxylated to total or intact osteocalcin in serum discriminates warfarin-treated patients from control subjects},
  url          = {http://dx.doi.org/10.1359/jbmr.1999.14.4.555},
  volume       = {14},
  year         = {1999},
}