Grafted neural stem cells develop into functional pyramidal neurons and integrate into host cortical circuitry.
Englund, Ulrica ; Björklund, Anders LU
; Wictorin, Klas
LU
; Lindvall, Olle
LU
and Kokaia, Merab
LU
(2002)
In Proceedings of the National Academy of Sciences
99(26).
p.17089-17094
- Abstract
- In vitro expanded neural stem/progenitor cells can undergo region-specific differentiation after transplantation to the developing or adult brain, and display morphologies and markers characteristic of mature neurons. Here we have used patch-clamp techniques to explore whether grafted stem cells also can develop physiological properties of mature neurons and become functionally integrated within host neural circuitry. The immortalized neural progenitor cell line, RN33B, prelabeled with GFP by using a lentiviral vector, was transplanted into the cortex or hippocampus of neonatal rats. We found that the grafted GFP-positive cells differentiated into cells with morphological features of cortical or hippocampal pyramidal neurons, and that many... (More)
- In vitro expanded neural stem/progenitor cells can undergo region-specific differentiation after transplantation to the developing or adult brain, and display morphologies and markers characteristic of mature neurons. Here we have used patch-clamp techniques to explore whether grafted stem cells also can develop physiological properties of mature neurons and become functionally integrated within host neural circuitry. The immortalized neural progenitor cell line, RN33B, prelabeled with GFP by using a lentiviral vector, was transplanted into the cortex or hippocampus of neonatal rats. We found that the grafted GFP-positive cells differentiated into cells with morphological features of cortical or hippocampal pyramidal neurons, and that many of them had established appropriate cortico-thalamic and contralateral hippocampal connections, respectively, as revealed by retrograde tracing. Whole-cell patch-clamp recordings from grafted cells with morphological characteristics of pyramidal neurons showed that they were able to generate action potentials, and received functional excitatory and inhibitory synaptic inputs from neighboring cells. These data provide evidence that grafted neural progenitors can differentiate into morphologically mature pyramidal projection neurons, establish appropriate long-distance axonal projections, exhibit normal electrophysiological properties, and become functionally integrated into host cortical circuitry. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/111530
- author
- Englund, Ulrica
; Björklund, Anders
LU
; Wictorin, Klas
LU
; Lindvall, Olle
LU
and Kokaia, Merab
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences
- volume
- 99
- issue
- 26
- pages
- 17089 - 17094
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000180101600104
- scopus:0037168599
- pmid:12471158
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.252589099
- language
- English
- LU publication?
- yes
- id
- 8e6fd87e-8f62-4302-9a74-f3522dab4287 (old id 111530)
- date added to LUP
- 2016-04-01 12:18:12
- date last changed
- 2022-01-27 01:45:09
@article{8e6fd87e-8f62-4302-9a74-f3522dab4287,
abstract = {{In vitro expanded neural stem/progenitor cells can undergo region-specific differentiation after transplantation to the developing or adult brain, and display morphologies and markers characteristic of mature neurons. Here we have used patch-clamp techniques to explore whether grafted stem cells also can develop physiological properties of mature neurons and become functionally integrated within host neural circuitry. The immortalized neural progenitor cell line, RN33B, prelabeled with GFP by using a lentiviral vector, was transplanted into the cortex or hippocampus of neonatal rats. We found that the grafted GFP-positive cells differentiated into cells with morphological features of cortical or hippocampal pyramidal neurons, and that many of them had established appropriate cortico-thalamic and contralateral hippocampal connections, respectively, as revealed by retrograde tracing. Whole-cell patch-clamp recordings from grafted cells with morphological characteristics of pyramidal neurons showed that they were able to generate action potentials, and received functional excitatory and inhibitory synaptic inputs from neighboring cells. These data provide evidence that grafted neural progenitors can differentiate into morphologically mature pyramidal projection neurons, establish appropriate long-distance axonal projections, exhibit normal electrophysiological properties, and become functionally integrated into host cortical circuitry.}},
author = {{Englund, Ulrica and Björklund, Anders and Wictorin, Klas and Lindvall, Olle and Kokaia, Merab}},
issn = {{1091-6490}},
language = {{eng}},
number = {{26}},
pages = {{17089--17094}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences}},
title = {{Grafted neural stem cells develop into functional pyramidal neurons and integrate into host cortical circuitry.}},
url = {{http://dx.doi.org/10.1073/pnas.252589099}},
doi = {{10.1073/pnas.252589099}},
volume = {{99}},
year = {{2002}},
}