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Macromolecular geometries determined with field-flow fractionation and their impact on the overlap concentration.

Rojas, Carola LU ; Wahlund, Karl-Gustav LU ; Bergenståhl, Björn LU and Nilsson, Lars LU (2008) In Biomacromolecules 9(6). p.1684-1690
Abstract
In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared... (More)
In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared with viscosimetry and discussed in terms of concentration and close-packing in relation to macromolecular shape and conformation. Hence, the results show that AsFlFFF and our novel methodology enable the determination of several physical properties with high relevance for the solution behavior of polydisperse macromolecules. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
9
issue
6
pages
1684 - 1690
publisher
The American Chemical Society
external identifiers
  • wos:000256635100024
  • pmid:18537296
  • scopus:46849106470
ISSN
1526-4602
DOI
10.1021/bm800127n
language
English
LU publication?
yes
id
cbe53809-67a6-4f85-be9c-eb476e8f8364 (old id 1169054)
date added to LUP
2009-03-09 11:43:48
date last changed
2017-01-01 05:01:57
@article{cbe53809-67a6-4f85-be9c-eb476e8f8364,
  abstract     = {In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared with viscosimetry and discussed in terms of concentration and close-packing in relation to macromolecular shape and conformation. Hence, the results show that AsFlFFF and our novel methodology enable the determination of several physical properties with high relevance for the solution behavior of polydisperse macromolecules.},
  author       = {Rojas, Carola and Wahlund, Karl-Gustav and Bergenståhl, Björn and Nilsson, Lars},
  issn         = {1526-4602},
  language     = {eng},
  number       = {6},
  pages        = {1684--1690},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Macromolecular geometries determined with field-flow fractionation and their impact on the overlap concentration.},
  url          = {http://dx.doi.org/10.1021/bm800127n},
  volume       = {9},
  year         = {2008},
}