Macromolecular geometries determined with field-flow fractionation and their impact on the overlap concentration.
Rojas, Carola LU ; Wahlund, Karl-Gustav LU ; Bergenståhl, Björn LU
and Nilsson, Lars
LU
(2008)
In Biomacromolecules
9(6).
p.1684-1690
- Abstract
- In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared... (More)
- In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared with viscosimetry and discussed in terms of concentration and close-packing in relation to macromolecular shape and conformation. Hence, the results show that AsFlFFF and our novel methodology enable the determination of several physical properties with high relevance for the solution behavior of polydisperse macromolecules. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1169054
- author
- Rojas, Carola
LU
; Wahlund, Karl-Gustav
LU
; Bergenståhl, Björn
LU
and Nilsson, Lars
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomacromolecules
- volume
- 9
- issue
- 6
- pages
- 1684 - 1690
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000256635100024
- pmid:18537296
- scopus:46849106470
- pmid:18537296
- ISSN
- 1526-4602
- DOI
- 10.1021/bm800127n
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Food Technology (011001017)
- id
- cbe53809-67a6-4f85-be9c-eb476e8f8364 (old id 1169054)
- date added to LUP
- 2016-04-01 12:18:45
- date last changed
- 2024-10-09 05:35:59
@article{cbe53809-67a6-4f85-be9c-eb476e8f8364,
abstract = {{In this paper we aim to understand the size/conformation relationship in waxy barley starch, a polydisperse and ultrahigh molar mass biomacromolecule. Characterizations are performed with asymmetrical flow field-flow fractionation (AsFlFFF). Furthermore, we study the effect of homogenization on the molar mass, rms radius (r rms) and hydrodynamic radius (r h). For the untreated sample, the macromolecules are elongated objects with low apparent density. As a result of homogenization, molar mass, and r rms decrease, while r h remains unaffected. The process also induces an increase, and scaling with size, of apparent density as well as changes in conformation, represented qualitatively by r rms/ r h. Finally, results from AsFlFFF are compared with viscosimetry and discussed in terms of concentration and close-packing in relation to macromolecular shape and conformation. Hence, the results show that AsFlFFF and our novel methodology enable the determination of several physical properties with high relevance for the solution behavior of polydisperse macromolecules.}},
author = {{Rojas, Carola and Wahlund, Karl-Gustav and Bergenståhl, Björn and Nilsson, Lars}},
issn = {{1526-4602}},
language = {{eng}},
number = {{6}},
pages = {{1684--1690}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Biomacromolecules}},
title = {{Macromolecular geometries determined with field-flow fractionation and their impact on the overlap concentration.}},
url = {{http://dx.doi.org/10.1021/bm800127n}},
doi = {{10.1021/bm800127n}},
volume = {{9}},
year = {{2008}},
}