Advanced

Exploring metabolic responses of potato tissue induced by electric pulses

Gomez, Federico LU ; Wadsö, Lars LU ; Vicente, A and Dejmek, Petr LU (2008) In Food Biophysics
Abstract
In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the... (More)
In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Metabolic stimulation, Isothermal calorimetry, Impedance, Electrical resistance
in
Food Biophysics
publisher
Springer
external identifiers
  • scopus:54249151917
ISSN
1557-1866
DOI
10.1007/s11483-008-9086-3
language
English
LU publication?
yes
id
eb1fd823-e637-44e1-b5d9-4ffceffc4222 (old id 1169697)
date added to LUP
2008-07-21 10:46:58
date last changed
2017-09-24 03:43:09
@article{eb1fd823-e637-44e1-b5d9-4ffceffc4222,
  abstract     = {In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress.},
  author       = {Gomez, Federico and Wadsö, Lars and Vicente, A and Dejmek, Petr},
  issn         = {1557-1866},
  keyword      = {Metabolic stimulation,Isothermal calorimetry,Impedance,Electrical resistance},
  language     = {eng},
  publisher    = {Springer},
  series       = {Food Biophysics},
  title        = {Exploring metabolic responses of potato tissue induced by electric pulses},
  url          = {http://dx.doi.org/10.1007/s11483-008-9086-3},
  year         = {2008},
}