Primary and secondary capture of platelets onto inflamed femoral artery endothelium is dependent on P-selectin and PSGL-1.
Braun, Oscar LU ; Slotta, Jan E ; Menger, Michael D ; Erlinge, David LU
and Thorlacius, Henrik
LU
(2008)
In European Journal of Pharmacology
592.
p.128-132
- Abstract
- Platelets constitute a key role in vascular injuries, however, the detailed mechanisms behind platelet-endothelial cell and platelet-leukocyte interactions in the femoral artery are not yet fully elucidated. We used intravital fluorescence microscopy of the femoral artery in C57BL/6 mice to study primary and secondary capture of platelets onto endothelial cells as well as onto adherent platelets and leukocytes in vivo. By use of monoclonal antibodies, the role of P-selectin and P-selectin glycoprotein ligand 1 (PSGL-1) in these adhesive interactions in mice exposed to endotoxin was determined. Intravenous injection of endotoxin significantly increased gene expression of P-selectin as well as platelet tethering, rolling and adhesion in the... (More)
- Platelets constitute a key role in vascular injuries, however, the detailed mechanisms behind platelet-endothelial cell and platelet-leukocyte interactions in the femoral artery are not yet fully elucidated. We used intravital fluorescence microscopy of the femoral artery in C57BL/6 mice to study primary and secondary capture of platelets onto endothelial cells as well as onto adherent platelets and leukocytes in vivo. By use of monoclonal antibodies, the role of P-selectin and P-selectin glycoprotein ligand 1 (PSGL-1) in these adhesive interactions in mice exposed to endotoxin was determined. Intravenous injection of endotoxin significantly increased gene expression of P-selectin as well as platelet tethering, rolling and adhesion in the femoral artery. Pretreatment with the anti-PSGL-1 antibody decreased platelet tethering by 85%, platelet rolling by 88% and platelet adhesion by 96%. Immunoneutralization of P-selectin reduced platelet tethering by 91%, platelet rolling by 98%, and platelet adhesion by 97%. In addition, inhibition of P-selectin and PSGL-1 completely abolished secondary capture of platelets onto adherent platelets and leukocytes. Our data show that P-selectin and PSGL-1 mediate early interactions between platelets and other cells, including endothelial cells and leukocytes, in inflamed arteries. These novel results suggest that interference with P-selectin and PSGL-1 may be a useful target in strategies aiming to protect the vascular wall during arterial inflammation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1180955
- author
- Braun, Oscar
LU
; Slotta, Jan E
; Menger, Michael D
; Erlinge, David
LU
and Thorlacius, Henrik
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Pharmacology
- volume
- 592
- pages
- 128 - 132
- publisher
- Elsevier
- external identifiers
-
- wos:000259434400021
- pmid:18644365
- scopus:49749144880
- ISSN
- 1879-0712
- DOI
- 10.1016/j.ejphar.2008.06.102
- language
- English
- LU publication?
- yes
- id
- 350aaee9-b7cc-41c6-9b7a-2141709b9dd6 (old id 1180955)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18644365?dopt=Abstract
- date added to LUP
- 2016-04-04 08:06:39
- date last changed
- 2022-01-29 02:59:55
@article{350aaee9-b7cc-41c6-9b7a-2141709b9dd6,
abstract = {{Platelets constitute a key role in vascular injuries, however, the detailed mechanisms behind platelet-endothelial cell and platelet-leukocyte interactions in the femoral artery are not yet fully elucidated. We used intravital fluorescence microscopy of the femoral artery in C57BL/6 mice to study primary and secondary capture of platelets onto endothelial cells as well as onto adherent platelets and leukocytes in vivo. By use of monoclonal antibodies, the role of P-selectin and P-selectin glycoprotein ligand 1 (PSGL-1) in these adhesive interactions in mice exposed to endotoxin was determined. Intravenous injection of endotoxin significantly increased gene expression of P-selectin as well as platelet tethering, rolling and adhesion in the femoral artery. Pretreatment with the anti-PSGL-1 antibody decreased platelet tethering by 85%, platelet rolling by 88% and platelet adhesion by 96%. Immunoneutralization of P-selectin reduced platelet tethering by 91%, platelet rolling by 98%, and platelet adhesion by 97%. In addition, inhibition of P-selectin and PSGL-1 completely abolished secondary capture of platelets onto adherent platelets and leukocytes. Our data show that P-selectin and PSGL-1 mediate early interactions between platelets and other cells, including endothelial cells and leukocytes, in inflamed arteries. These novel results suggest that interference with P-selectin and PSGL-1 may be a useful target in strategies aiming to protect the vascular wall during arterial inflammation.}},
author = {{Braun, Oscar and Slotta, Jan E and Menger, Michael D and Erlinge, David and Thorlacius, Henrik}},
issn = {{1879-0712}},
language = {{eng}},
pages = {{128--132}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmacology}},
title = {{Primary and secondary capture of platelets onto inflamed femoral artery endothelium is dependent on P-selectin and PSGL-1.}},
url = {{http://dx.doi.org/10.1016/j.ejphar.2008.06.102}},
doi = {{10.1016/j.ejphar.2008.06.102}},
volume = {{592}},
year = {{2008}},
}