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Determination of heterocyclic aromatic amines in human urine by using hollow-fibre supported liquid membrane extraction and liquid chromatography-ultraviolet detection system.

Ullah, Faiz LU ; Barri, Thaer LU ; Jönsson, Jan Åke LU and Skog, Kerstin LU (2008) In Journal of Chromatography. B 870(2). p.203-208
Abstract
A hollow-fibre supported liquid membrane (HF-SLM) extraction method has been developed for determination of 11 heterocyclic aromatic amines (HCAs) in human urine samples by using high performance liquid chromatography (HPLC) equipped with an ultraviolet (UV) absorbance detector. These compounds were extracted from an alkaline urine sample (donor phase) into the organic solvent residing in the pores of a polypropylene hollow fibre and then back extracted into an acidic solution (acceptor phase) inside the lumen of the hollow fibre. After extraction, HCAs were analyzed by injecting the analyte enriched acceptor phase into the HPLC. The analyte enrichment factors ranged between 241 and 339 obtained in a 90min extraction time, and method... (More)
A hollow-fibre supported liquid membrane (HF-SLM) extraction method has been developed for determination of 11 heterocyclic aromatic amines (HCAs) in human urine samples by using high performance liquid chromatography (HPLC) equipped with an ultraviolet (UV) absorbance detector. These compounds were extracted from an alkaline urine sample (donor phase) into the organic solvent residing in the pores of a polypropylene hollow fibre and then back extracted into an acidic solution (acceptor phase) inside the lumen of the hollow fibre. After extraction, HCAs were analyzed by injecting the analyte enriched acceptor phase into the HPLC. The analyte enrichment factors ranged between 241 and 339 obtained in a 90min extraction time, and method detection limits (MDL) ranged between 0.1 and 0.5mugL(-1) with relative standard deviation (RSD) values between 3.4% and 11%. The extraction technique employed in this work is easy to use and rapid as it involves only a few minutes manipulation of each sample. It is the most economical sample preparation/preconcentration technique to our knowledge as compared to other microextraction techniques. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
High performance liquid chromatography with ultraviolet detector, Heterocyclic aromatic amines, Urine sample, Hollow-fibre supported liquid membrane
in
Journal of Chromatography. B
volume
870
issue
2
pages
203 - 208
publisher
Elsevier
external identifiers
  • wos:000258388600011
  • pmid:18599369
  • scopus:47249157569
ISSN
1873-376X
DOI
10.1016/j.jchromb.2008.06.006
language
English
LU publication?
yes
id
df39cf10-ab12-49a1-97b0-2ae5e473107b (old id 1181567)
date added to LUP
2008-10-17 11:21:50
date last changed
2017-07-09 03:36:29
@article{df39cf10-ab12-49a1-97b0-2ae5e473107b,
  abstract     = {A hollow-fibre supported liquid membrane (HF-SLM) extraction method has been developed for determination of 11 heterocyclic aromatic amines (HCAs) in human urine samples by using high performance liquid chromatography (HPLC) equipped with an ultraviolet (UV) absorbance detector. These compounds were extracted from an alkaline urine sample (donor phase) into the organic solvent residing in the pores of a polypropylene hollow fibre and then back extracted into an acidic solution (acceptor phase) inside the lumen of the hollow fibre. After extraction, HCAs were analyzed by injecting the analyte enriched acceptor phase into the HPLC. The analyte enrichment factors ranged between 241 and 339 obtained in a 90min extraction time, and method detection limits (MDL) ranged between 0.1 and 0.5mugL(-1) with relative standard deviation (RSD) values between 3.4% and 11%. The extraction technique employed in this work is easy to use and rapid as it involves only a few minutes manipulation of each sample. It is the most economical sample preparation/preconcentration technique to our knowledge as compared to other microextraction techniques.},
  author       = {Ullah, Faiz and Barri, Thaer and Jönsson, Jan Åke and Skog, Kerstin},
  issn         = {1873-376X},
  keyword      = {High performance liquid chromatography with ultraviolet detector,Heterocyclic aromatic amines,Urine sample,Hollow-fibre supported liquid membrane},
  language     = {eng},
  number       = {2},
  pages        = {203--208},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Determination of heterocyclic aromatic amines in human urine by using hollow-fibre supported liquid membrane extraction and liquid chromatography-ultraviolet detection system.},
  url          = {http://dx.doi.org/10.1016/j.jchromb.2008.06.006},
  volume       = {870},
  year         = {2008},
}