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Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens

Hirai, Hiroki; Miura, Junnosuke; Hu, Yafang; Larsson, Helena LU ; Larsson, Karin LU ; Lernmark, Åke LU ; Ivarsson, Sten LU ; Wu, Tianxia; Kingman, Albert and Tzioufas, Athanasios G, et al. (2008) In Clinical Immunology 127(3). p.366-374
Abstract
The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory... (More)
The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases. (Less)
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publication status
published
subject
keywords
type 1 diabetes, secretory vesicles, proteome, phosphatase, protein tyrosine, IA-2, GAD65, autoantibodies, autoantigens
in
Clinical Immunology
volume
127
issue
3
pages
366 - 374
publisher
Elsevier
external identifiers
  • wos:000256373600014
  • scopus:45249111131
ISSN
1521-6616
DOI
10.1016/j.clim.2008.01.018
language
English
LU publication?
yes
id
0553cb5f-f2bb-40ca-9421-032dd731a8cc (old id 1201432)
date added to LUP
2008-09-15 10:15:00
date last changed
2017-07-23 03:36:26
@article{0553cb5f-f2bb-40ca-9421-032dd731a8cc,
  abstract     = {The four major autoantigens (IA-2, IA-2 beta, GAD65 and insulin) of type 1 diabetes are all associated with dense core or synaptic vesicles. This raised the possibility that other secretory vesicle-associated proteins might be targets of the autoimmune response in type 1 diabetes. To test this hypothesis 56 proteins, two-thirds of which are associated with secretory vesicles, were prepared by in vitro transcription/translation and screened for autoantibodies by liquid phase radioimmunoprecipitation. Two secretory vesicle-associated proteins, VAMP2 and NPY, were identified as new minor autoantigens with 21% and 9%, respectively, of 200 type 1 diabetes sera reacting positively. These findings add support to the hypothesis that secretory vesicle-associated proteins are particularly important, but not the exclusive, targets of the autoimmune response in type 1 diabetes. Selective screening of the human proteome offers a useful approach for identifying new autoantigens in autoimmune diseases.},
  author       = {Hirai, Hiroki and Miura, Junnosuke and Hu, Yafang and Larsson, Helena and Larsson, Karin and Lernmark, Åke and Ivarsson, Sten and Wu, Tianxia and Kingman, Albert and Tzioufas, Athanasios G and Notkins, Abner L},
  issn         = {1521-6616},
  keyword      = {type 1 diabetes,secretory vesicles,proteome,phosphatase,protein tyrosine,IA-2,GAD65,autoantibodies,autoantigens},
  language     = {eng},
  number       = {3},
  pages        = {366--374},
  publisher    = {Elsevier},
  series       = {Clinical Immunology},
  title        = {Selective screening of secretory vesicle-associated proteins for autoantigens in type 1 diabetes: VAMP2 and NPY are new minor autoantigens},
  url          = {http://dx.doi.org/10.1016/j.clim.2008.01.018},
  volume       = {127},
  year         = {2008},
}