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The enzyme levels in blood are not affected by oral administration of a pancreatic enzyme preparation (Creon 10,000) in pancreas-insufficient pigs.

Gewert, Karin; Holowachuk, Scott A; Rippe, Catarina LU ; Gregory, Peter C; Erlanson-Albertsson, Charlotte; Olivecrona, Gunilla; Kruszewska, Danuta LU ; Piedra, Jose Valverde; Weström, Björn LU and Pierzynowski, Stefan LU (2004) In Pancreas 28(1). p.80-88
Abstract
After oral intake, small amounts of intact protein may be absorbed into the blood circulation. The current study investigated whether orally administered pancreatic enzymes were absorbed from the intestine. The study included 28 pigs; 3 control pigs with intact pancreatic function and 25 pigs that were made exocrine pancreas insufficient by duct ligation (20 pigs) or total pancreatectomy (5 pigs).The pigs received a pancreatic enzyme preparation (0, 2, 4, or 8 g of Creon 10,000) together with the feed. The blood plasma was analyzed for pancreatic lipase activity with a [3H]-triolein substrate assay, while (pro)colipase and cationic trypsin(ogen) levels were measured with enzyme-linked immunosorbent assay (ELISA). Administration of Creon... (More)
After oral intake, small amounts of intact protein may be absorbed into the blood circulation. The current study investigated whether orally administered pancreatic enzymes were absorbed from the intestine. The study included 28 pigs; 3 control pigs with intact pancreatic function and 25 pigs that were made exocrine pancreas insufficient by duct ligation (20 pigs) or total pancreatectomy (5 pigs).The pigs received a pancreatic enzyme preparation (0, 2, 4, or 8 g of Creon 10,000) together with the feed. The blood plasma was analyzed for pancreatic lipase activity with a [3H]-triolein substrate assay, while (pro)colipase and cationic trypsin(ogen) levels were measured with enzyme-linked immunosorbent assay (ELISA). Administration of Creon (0-8 g) caused no significant changes in plasma (pro)colipase or cationic trypsin(ogen) levels. Lipase activity peaks in plasma samples were found, but they did not correspond to the administration of Creon. The potential source of these plasma lipase activity peaks is discussed. The results showed no absorption into blood of pancreatic enzymes after oral administration (0, 2, 4, or 8 g of Creon mixed with 100 g of feed) to pancreas-insufficient pigs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pancreas
volume
28
issue
1
pages
80 - 88
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:14707735
  • wos:000187840800013
  • scopus:9144229785
ISSN
0885-3177
language
English
LU publication?
yes
id
1be0b32c-29fe-4676-9736-9faf59200f42 (old id 120310)
alternative location
http://www.pancreasjournal.com/pt/re/pancreas/abstract.00006676-200401000-00013.htm
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=14707735&ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum
date added to LUP
2007-06-28 13:39:27
date last changed
2017-11-05 03:48:32
@article{1be0b32c-29fe-4676-9736-9faf59200f42,
  abstract     = {After oral intake, small amounts of intact protein may be absorbed into the blood circulation. The current study investigated whether orally administered pancreatic enzymes were absorbed from the intestine. The study included 28 pigs; 3 control pigs with intact pancreatic function and 25 pigs that were made exocrine pancreas insufficient by duct ligation (20 pigs) or total pancreatectomy (5 pigs).The pigs received a pancreatic enzyme preparation (0, 2, 4, or 8 g of Creon 10,000) together with the feed. The blood plasma was analyzed for pancreatic lipase activity with a [3H]-triolein substrate assay, while (pro)colipase and cationic trypsin(ogen) levels were measured with enzyme-linked immunosorbent assay (ELISA). Administration of Creon (0-8 g) caused no significant changes in plasma (pro)colipase or cationic trypsin(ogen) levels. Lipase activity peaks in plasma samples were found, but they did not correspond to the administration of Creon. The potential source of these plasma lipase activity peaks is discussed. The results showed no absorption into blood of pancreatic enzymes after oral administration (0, 2, 4, or 8 g of Creon mixed with 100 g of feed) to pancreas-insufficient pigs.},
  author       = {Gewert, Karin and Holowachuk, Scott A and Rippe, Catarina and Gregory, Peter C and Erlanson-Albertsson, Charlotte and Olivecrona, Gunilla and Kruszewska, Danuta and Piedra, Jose Valverde and Weström, Björn and Pierzynowski, Stefan},
  issn         = {0885-3177},
  language     = {eng},
  number       = {1},
  pages        = {80--88},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Pancreas},
  title        = {The enzyme levels in blood are not affected by oral administration of a pancreatic enzyme preparation (Creon 10,000) in pancreas-insufficient pigs.},
  volume       = {28},
  year         = {2004},
}