Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system
(1996) In Applied Microbiology and Biotechnology 45(6). p.737-743- Abstract
- Abstract (1/2-em) In order to enhance the productivity of lactic acid and reduce the end-product inhibition of fermentation, the partitioning and growth of four different strains of lactic acid bacteria in three different aqueous two-phase systems were studied. Polyethyleneglycol/ dextran, polyethyleneglycol/hydroxypropyl starch polymer (HPS), and a random copolymer of ethylene oxide and propylene oxide (EO-PO)/HPS were used as polymer systems. One strain each of Lactococcus lactis subsp. lactis and of Lactobacillus delbrueckii subsp. delbrueckii partitioned completely to the interface and bottom phase in two-phase systems with low polymer concentrations of EO-PO/HPS100 and EO-PO/ HPS200. The growth and production of lactic acid by two of... (More)
- Abstract (1/2-em) In order to enhance the productivity of lactic acid and reduce the end-product inhibition of fermentation, the partitioning and growth of four different strains of lactic acid bacteria in three different aqueous two-phase systems were studied. Polyethyleneglycol/ dextran, polyethyleneglycol/hydroxypropyl starch polymer (HPS), and a random copolymer of ethylene oxide and propylene oxide (EO-PO)/HPS were used as polymer systems. One strain each of Lactococcus lactis subsp. lactis and of Lactobacillus delbrueckii subsp. delbrueckii partitioned completely to the interface and bottom phase in two-phase systems with low polymer concentrations of EO-PO/HPS100 and EO-PO/ HPS200. The growth and production of lactic acid by two of three L. lactis strains in a two-phase system with 5.5% (w/w) EO-PO and 12.0% (w/w) HPS100 were reduced by less than 10% compared with a reference fermentation in a normal growth medium. The viability of L. lactis subsp. lactis ATCC 19435 was maintained for at least 501/4-em h and with four top-phase replacements during extractive fermentation in the EO-PO/HPS100 system. Moreover, when cell density reached the stationary phase in the first extractive fermentation, the lactate production in this aqueous two-phase system was maintained. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/126338
- author
- Planas, J ; Rådström, Peter LU ; Tjerneld, Folke LU and Hahn-Hägerdal, Bärbel LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Microbiology and Biotechnology
- volume
- 45
- issue
- 6
- pages
- 737 - 743
- publisher
- Springer
- external identifiers
-
- scopus:0029742390
- ISSN
- 1432-0614
- DOI
- 10.1007/s002530050756
- language
- English
- LU publication?
- yes
- id
- 5edda2d4-639f-4a89-8e6f-d81db79669d1 (old id 126338)
- date added to LUP
- 2016-04-01 15:21:47
- date last changed
- 2022-01-28 04:59:39
@article{5edda2d4-639f-4a89-8e6f-d81db79669d1, abstract = {{Abstract (1/2-em) In order to enhance the productivity of lactic acid and reduce the end-product inhibition of fermentation, the partitioning and growth of four different strains of lactic acid bacteria in three different aqueous two-phase systems were studied. Polyethyleneglycol/ dextran, polyethyleneglycol/hydroxypropyl starch polymer (HPS), and a random copolymer of ethylene oxide and propylene oxide (EO-PO)/HPS were used as polymer systems. One strain each of Lactococcus lactis subsp. lactis and of Lactobacillus delbrueckii subsp. delbrueckii partitioned completely to the interface and bottom phase in two-phase systems with low polymer concentrations of EO-PO/HPS100 and EO-PO/ HPS200. The growth and production of lactic acid by two of three L. lactis strains in a two-phase system with 5.5% (w/w) EO-PO and 12.0% (w/w) HPS100 were reduced by less than 10% compared with a reference fermentation in a normal growth medium. The viability of L. lactis subsp. lactis ATCC 19435 was maintained for at least 501/4-em h and with four top-phase replacements during extractive fermentation in the EO-PO/HPS100 system. Moreover, when cell density reached the stationary phase in the first extractive fermentation, the lactate production in this aqueous two-phase system was maintained.}}, author = {{Planas, J and Rådström, Peter and Tjerneld, Folke and Hahn-Hägerdal, Bärbel}}, issn = {{1432-0614}}, language = {{eng}}, number = {{6}}, pages = {{737--743}}, publisher = {{Springer}}, series = {{Applied Microbiology and Biotechnology}}, title = {{Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system}}, url = {{http://dx.doi.org/10.1007/s002530050756}}, doi = {{10.1007/s002530050756}}, volume = {{45}}, year = {{1996}}, }