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Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system

Planas, J; Rådström, Peter LU ; Tjerneld, Folke LU and Hahn-Hägerdal, Bärbel LU (1996) In Applied Microbiology and Biotechnology 45(6). p.737-743
Abstract
Abstract (1/2-em) In order to enhance the productivity of lactic acid and reduce the end-product inhibition of fermentation, the partitioning and growth of four different strains of lactic acid bacteria in three different aqueous two-phase systems were studied. Polyethyleneglycol/ dextran, polyethyleneglycol/hydroxypropyl starch polymer (HPS), and a random copolymer of ethylene oxide and propylene oxide (EO-PO)/HPS were used as polymer systems. One strain each of Lactococcus lactis subsp. lactis and of Lactobacillus delbrueckii subsp. delbrueckii partitioned completely to the interface and bottom phase in two-phase systems with low polymer concentrations of EO-PO/HPS100 and EO-PO/ HPS200. The growth and production of lactic acid by two of... (More)
Abstract (1/2-em) In order to enhance the productivity of lactic acid and reduce the end-product inhibition of fermentation, the partitioning and growth of four different strains of lactic acid bacteria in three different aqueous two-phase systems were studied. Polyethyleneglycol/ dextran, polyethyleneglycol/hydroxypropyl starch polymer (HPS), and a random copolymer of ethylene oxide and propylene oxide (EO-PO)/HPS were used as polymer systems. One strain each of Lactococcus lactis subsp. lactis and of Lactobacillus delbrueckii subsp. delbrueckii partitioned completely to the interface and bottom phase in two-phase systems with low polymer concentrations of EO-PO/HPS100 and EO-PO/ HPS200. The growth and production of lactic acid by two of three L. lactis strains in a two-phase system with 5.5% (w/w) EO-PO and 12.0% (w/w) HPS100 were reduced by less than 10% compared with a reference fermentation in a normal growth medium. The viability of L. lactis subsp. lactis ATCC 19435 was maintained for at least 501/4-em h and with four top-phase replacements during extractive fermentation in the EO-PO/HPS100 system. Moreover, when cell density reached the stationary phase in the first extractive fermentation, the lactate production in this aqueous two-phase system was maintained. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
45
issue
6
pages
737 - 743
publisher
Springer
external identifiers
  • scopus:0029742390
ISSN
1432-0614
DOI
10.1007/s002530050756
language
English
LU publication?
yes
id
5edda2d4-639f-4a89-8e6f-d81db79669d1 (old id 126338)
date added to LUP
2007-06-28 16:41:06
date last changed
2017-01-01 06:38:15
@article{5edda2d4-639f-4a89-8e6f-d81db79669d1,
  abstract     = {Abstract (1/2-em) In order to enhance the productivity of lactic acid and reduce the end-product inhibition of fermentation, the partitioning and growth of four different strains of lactic acid bacteria in three different aqueous two-phase systems were studied. Polyethyleneglycol/ dextran, polyethyleneglycol/hydroxypropyl starch polymer (HPS), and a random copolymer of ethylene oxide and propylene oxide (EO-PO)/HPS were used as polymer systems. One strain each of Lactococcus lactis subsp. lactis and of Lactobacillus delbrueckii subsp. delbrueckii partitioned completely to the interface and bottom phase in two-phase systems with low polymer concentrations of EO-PO/HPS100 and EO-PO/ HPS200. The growth and production of lactic acid by two of three L. lactis strains in a two-phase system with 5.5% (w/w) EO-PO and 12.0% (w/w) HPS100 were reduced by less than 10% compared with a reference fermentation in a normal growth medium. The viability of L. lactis subsp. lactis ATCC 19435 was maintained for at least 501/4-em h and with four top-phase replacements during extractive fermentation in the EO-PO/HPS100 system. Moreover, when cell density reached the stationary phase in the first extractive fermentation, the lactate production in this aqueous two-phase system was maintained.},
  author       = {Planas, J and Rådström, Peter and Tjerneld, Folke and Hahn-Hägerdal, Bärbel},
  issn         = {1432-0614},
  language     = {eng},
  number       = {6},
  pages        = {737--743},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Enhanced production of lactic acid through the use of a novel aqueous two-phase system as an extractive fermentation system},
  url          = {http://dx.doi.org/10.1007/s002530050756},
  volume       = {45},
  year         = {1996},
}