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Regulation of the eicosanoid pathway by tumour necrosis factor alpha and leukotriene D(4) in intestinal epithelial cells.

Yudina, Yulyana LU ; Parhamifar, Ladan LU ; Bengtsson, Astrid LU ; Juhas, Maria LU and Sjölander, Anita LU (2008) In Prostaglandins, Leukotrienes and Essential Fatty Acids 79(6). p.223-231
Abstract
In this study the mRNA and protein levels of the key enzymes involved in eicosanoid biosynthesis and the cysteinyl leukotriene receptors (CysLT(1)R and CysLT(2)R) have been analysed in non-transformed intestinal epithelial and colon cancer cell lines. Our results revealed that tumour necrosis factor alpha (TNF-alpha), and leukotriene D(4) (LTD(4)), which are inflammatory mediators implicated in carcinogenesis, stimulated an increase of cyclooxygenase-2 (COX-2), in non-transformed epithelial cells, and 5-lipoxygenase (5-LO) in both non-transformed and cancer cell lines. Furthermore, these mediators also stimulated an up-regulation of LTC(4) synthase in cancer cells as well as non-transformed cells. We also observed an endogenous production... (More)
In this study the mRNA and protein levels of the key enzymes involved in eicosanoid biosynthesis and the cysteinyl leukotriene receptors (CysLT(1)R and CysLT(2)R) have been analysed in non-transformed intestinal epithelial and colon cancer cell lines. Our results revealed that tumour necrosis factor alpha (TNF-alpha), and leukotriene D(4) (LTD(4)), which are inflammatory mediators implicated in carcinogenesis, stimulated an increase of cyclooxygenase-2 (COX-2), in non-transformed epithelial cells, and 5-lipoxygenase (5-LO) in both non-transformed and cancer cell lines. Furthermore, these mediators also stimulated an up-regulation of LTC(4) synthase in cancer cells as well as non-transformed cells. We also observed an endogenous production of CysLTs in these cells. TNF-alpha and LTD(4), to a lesser extent, up-regulate the CysLT(1)R levels. Interestingly, TNF-alpha also reduced CysLT(2)R expression in cancer cells. Our results demonstrate that inflammatory mediators can cause intestinal epithelial cells to up-regulate the expression of enzymes needed for the biosynthesis of eicosanoids, including the cysteinyl leukotrienes, as well as the signal transducing proteins, the CysLT receptors, thus providing important mechanisms for both maintaining inflammation and for tumour progression. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Prostaglandins, Leukotrienes and Essential Fatty Acids
volume
79
issue
6
pages
223 - 231
publisher
Elsevier
external identifiers
  • wos:000261994800006
  • pmid:19042113
  • scopus:61549132288
ISSN
0952-3278
DOI
10.1016/j.plefa.2008.09.024
language
English
LU publication?
yes
id
1a887eae-d94a-4591-afd8-1e3c1e1a03d8 (old id 1276653)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19042113?dopt=Abstract
date added to LUP
2009-01-09 08:42:16
date last changed
2017-01-01 07:33:51
@article{1a887eae-d94a-4591-afd8-1e3c1e1a03d8,
  abstract     = {In this study the mRNA and protein levels of the key enzymes involved in eicosanoid biosynthesis and the cysteinyl leukotriene receptors (CysLT(1)R and CysLT(2)R) have been analysed in non-transformed intestinal epithelial and colon cancer cell lines. Our results revealed that tumour necrosis factor alpha (TNF-alpha), and leukotriene D(4) (LTD(4)), which are inflammatory mediators implicated in carcinogenesis, stimulated an increase of cyclooxygenase-2 (COX-2), in non-transformed epithelial cells, and 5-lipoxygenase (5-LO) in both non-transformed and cancer cell lines. Furthermore, these mediators also stimulated an up-regulation of LTC(4) synthase in cancer cells as well as non-transformed cells. We also observed an endogenous production of CysLTs in these cells. TNF-alpha and LTD(4), to a lesser extent, up-regulate the CysLT(1)R levels. Interestingly, TNF-alpha also reduced CysLT(2)R expression in cancer cells. Our results demonstrate that inflammatory mediators can cause intestinal epithelial cells to up-regulate the expression of enzymes needed for the biosynthesis of eicosanoids, including the cysteinyl leukotrienes, as well as the signal transducing proteins, the CysLT receptors, thus providing important mechanisms for both maintaining inflammation and for tumour progression.},
  author       = {Yudina, Yulyana and Parhamifar, Ladan and Bengtsson, Astrid and Juhas, Maria and Sjölander, Anita},
  issn         = {0952-3278},
  language     = {eng},
  number       = {6},
  pages        = {223--231},
  publisher    = {Elsevier},
  series       = {Prostaglandins, Leukotrienes and Essential Fatty Acids},
  title        = {Regulation of the eicosanoid pathway by tumour necrosis factor alpha and leukotriene D(4) in intestinal epithelial cells.},
  url          = {http://dx.doi.org/10.1016/j.plefa.2008.09.024},
  volume       = {79},
  year         = {2008},
}