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A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol

Nistor, Catalin LU and Emnéus, Jenny LU (2003) In Analytical and Bioanalytical Chemistry 375(1). p.125-132
Abstract
This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection... (More)
This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl-#-D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical and Bioanalytical Chemistry
volume
375
issue
1
pages
125 - 132
publisher
Springer
external identifiers
  • pmid:12520448
  • wos:000180848800030
  • scopus:0037268719
ISSN
1618-2642
DOI
language
English
LU publication?
yes
id
89f9bdd4-2275-4fb1-aac5-77f3e3fc9708 (old id 128960)
date added to LUP
2007-06-27 13:40:40
date last changed
2018-05-29 10:37:16
@article{89f9bdd4-2275-4fb1-aac5-77f3e3fc9708,
  abstract     = {This paper describes the development of two different capillary-based heterogeneous competitive flow immunoassay formats (capillary flow injection immunoassay (CFIIA) and capillary sequential injection immunoassay (CSIIA)) for the determination of 2,4,6-trichlorophenol (2,4,6-TCP). The assays are based on the competition between the analyte and an analyte derivative labelled with the enzyme #-galactosidase, for an anti-TCP antibody, followed by the injection of the mixture at equilibrium into a flow stream, where separation between the fractions bound and unbound to the antibody is performed in a glass capillary containing immobilised protein A. The antibody-tracer fraction retained inside the protein A capillary was measured by injection of 4-aminophenyl-#-D-galactoside (4-APG), followed by amperometric detection of the enzymatically generated 4-aminophenol (4-AP), leading to a negative correlation between the signal and the analyte concentration.},
  author       = {Nistor, Catalin and Emnéus, Jenny},
  issn         = {1618-2642},
  language     = {eng},
  number       = {1},
  pages        = {125--132},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {A capillary-based amperometric flow immunoassay for 2,4,6-trichlorophenol},
  url          = {http://dx.doi.org/},
  volume       = {375},
  year         = {2003},
}