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Valorization of Brewer's spent grain to prebiotic oligosaccharide : Production, xylanase catalyzed hydrolysis, in-vitro evaluation with probiotic strains and in a batch human fecal fermentation model

Sajib, Mursalin; Falck, Peter LU ; Sardari, Roya R.R. LU ; Mathew, Sindhu; Grey, Carl LU ; Karlsson, Eva Nordberg LU and Adlercreutz, Patrick LU (2018) In Journal of Biotechnology 268. p.61-70
Abstract

Brewer's spent grain (BSG) accounts for around 85% of the solid by-products from beer production. BSG was first extracted to obtain water-soluble arabinoxylan (AX). Using subsequent alkali extraction (0.5 M KOH) it was possible to dissolve additional AX. In total, about 57% of the AX in BSG was extracted with the purity of 45–55%. After comparison of nine xylanases, Pentopan mono BG, a GH11 enzyme, was selected for hydrolysis of the extracts to oligosaccharides with minimal formation of monosaccharides. Growth of Bifidobacterium adolescentis (ATCC 15703) was promoted by the enzymatic hydrolysis to arabinoxylooligosaccharides, while Lactobacillus brevis (DSMZ 1264) utilized only unsubstituted xylooligosaccharides. Furthermore,... (More)

Brewer's spent grain (BSG) accounts for around 85% of the solid by-products from beer production. BSG was first extracted to obtain water-soluble arabinoxylan (AX). Using subsequent alkali extraction (0.5 M KOH) it was possible to dissolve additional AX. In total, about 57% of the AX in BSG was extracted with the purity of 45–55%. After comparison of nine xylanases, Pentopan mono BG, a GH11 enzyme, was selected for hydrolysis of the extracts to oligosaccharides with minimal formation of monosaccharides. Growth of Bifidobacterium adolescentis (ATCC 15703) was promoted by the enzymatic hydrolysis to arabinoxylooligosaccharides, while Lactobacillus brevis (DSMZ 1264) utilized only unsubstituted xylooligosaccharides. Furthermore, utilization of the hydrolysates by human gut microbiota was also assessed in a batch human fecal fermentation model. Results revealed that the rates of fermentation of the BSG hydrolysates by human gut microbiota were similar to that of commercial prebiotic fructooligosaccharides, while inulin was fermented at a slower rate. In summary, a sustainable process to valorize BSG to functional food ingredients has been proposed.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arabinoxylan, Arabinoxylooligosaccharides, Bifidobacterium adolescentis, Brewer's spent grain, Xylanase
in
Journal of Biotechnology
volume
268
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:85041352261
ISSN
0168-1656
DOI
10.1016/j.jbiotec.2018.01.005
language
English
LU publication?
yes
id
12da11ff-2908-46d4-b289-46f5408fcbfe
date added to LUP
2018-02-12 07:39:47
date last changed
2018-05-29 10:30:10
@article{12da11ff-2908-46d4-b289-46f5408fcbfe,
  abstract     = {<p>Brewer's spent grain (BSG) accounts for around 85% of the solid by-products from beer production. BSG was first extracted to obtain water-soluble arabinoxylan (AX). Using subsequent alkali extraction (0.5 M KOH) it was possible to dissolve additional AX. In total, about 57% of the AX in BSG was extracted with the purity of 45–55%. After comparison of nine xylanases, Pentopan mono BG, a GH11 enzyme, was selected for hydrolysis of the extracts to oligosaccharides with minimal formation of monosaccharides. Growth of Bifidobacterium adolescentis (ATCC 15703) was promoted by the enzymatic hydrolysis to arabinoxylooligosaccharides, while Lactobacillus brevis (DSMZ 1264) utilized only unsubstituted xylooligosaccharides. Furthermore, utilization of the hydrolysates by human gut microbiota was also assessed in a batch human fecal fermentation model. Results revealed that the rates of fermentation of the BSG hydrolysates by human gut microbiota were similar to that of commercial prebiotic fructooligosaccharides, while inulin was fermented at a slower rate. In summary, a sustainable process to valorize BSG to functional food ingredients has been proposed.</p>},
  author       = {Sajib, Mursalin and Falck, Peter and Sardari, Roya R.R. and Mathew, Sindhu and Grey, Carl and Karlsson, Eva Nordberg and Adlercreutz, Patrick},
  issn         = {0168-1656},
  keyword      = {Arabinoxylan,Arabinoxylooligosaccharides,Bifidobacterium adolescentis,Brewer's spent grain,Xylanase},
  language     = {eng},
  month        = {02},
  pages        = {61--70},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Valorization of Brewer's spent grain to prebiotic oligosaccharide : Production, xylanase catalyzed hydrolysis, in-vitro evaluation with probiotic strains and in a batch human fecal fermentation model},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2018.01.005},
  volume       = {268},
  year         = {2018},
}