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Arabidopsis genes encoding mitochondrial type II NAD(P)H dehydrogenases have different evolutionary orgin and show distinct responses to light.

Michalecka, Agnieszka LU ; Svensson, Staffan LU ; Johansson, Fredrik I LU ; Agius, Stephanie C; Johanson, Urban LU ; Brennicke, Axel; Binder, Stefan and Rasmusson, Allan LU (2003) In Plant Physiology 133(2). p.642-652
Abstract
In addition to proton-pumping complex I, plant mitochondria contain several type II NAD(P)H dehydrogenases in the electron transport chain. The extra enzymes allow the nonenergy-conserving electron transfer from cytoplasmic and matrix NAD(P)H to ubiquinone. We have investigated the type II NAD(P)H dehydrogenase gene families in Arabidopsis. This model plant contains two and four genes closely related to potato (Solanum tuberosum) genes nda1 and ndb1, respectively. A novel homolog, termed ndc1, with a lower but significant similarity to potato nda1 and ndb1, is also present. All genes are expressed in several organs of the plant. Among the nda genes, expression of nda1, but not nda2, is dependent on light and circadian regulation,... (More)
In addition to proton-pumping complex I, plant mitochondria contain several type II NAD(P)H dehydrogenases in the electron transport chain. The extra enzymes allow the nonenergy-conserving electron transfer from cytoplasmic and matrix NAD(P)H to ubiquinone. We have investigated the type II NAD(P)H dehydrogenase gene families in Arabidopsis. This model plant contains two and four genes closely related to potato (Solanum tuberosum) genes nda1 and ndb1, respectively. A novel homolog, termed ndc1, with a lower but significant similarity to potato nda1 and ndb1, is also present. All genes are expressed in several organs of the plant. Among the nda genes, expression of nda1, but not nda2, is dependent on light and circadian regulation, suggesting separate roles in photosynthesis-associated and other respiratory NADH oxidation. Genes from all three gene families encode proteins exclusively targeted to mitochondria, as revealed by expression of green fluorescent fusion proteins and by western blotting of fractionated cells. Phylogenetic analysis indicates that ndc1 affiliates with cyanobacterial type II NADH dehydrogenase genes, suggesting that this gene entered the eukaryotic cell via the chloroplast progenitor. The ndc1 should then have been transferred to the nucleus and acquired a signal for mitochondrial targeting of the protein product. Although they are of different origin, the nda, ndb, and ndc genes carry an identical intron position. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Physiology
volume
133
issue
2
pages
642 - 652
publisher
American Society of Plant Biologists
external identifiers
  • wos:000185974800026
  • pmid:12972666
  • scopus:0142152465
ISSN
1532-2548
DOI
language
English
LU publication?
yes
id
ad308f1c-1fcb-4e7d-b177-d6b503be2e7e (old id 132690)
date added to LUP
2007-07-06 12:26:04
date last changed
2018-05-29 11:44:19
@article{ad308f1c-1fcb-4e7d-b177-d6b503be2e7e,
  abstract     = {In addition to proton-pumping complex I, plant mitochondria contain several type II NAD(P)H dehydrogenases in the electron transport chain. The extra enzymes allow the nonenergy-conserving electron transfer from cytoplasmic and matrix NAD(P)H to ubiquinone. We have investigated the type II NAD(P)H dehydrogenase gene families in Arabidopsis. This model plant contains two and four genes closely related to potato (Solanum tuberosum) genes nda1 and ndb1, respectively. A novel homolog, termed ndc1, with a lower but significant similarity to potato nda1 and ndb1, is also present. All genes are expressed in several organs of the plant. Among the nda genes, expression of nda1, but not nda2, is dependent on light and circadian regulation, suggesting separate roles in photosynthesis-associated and other respiratory NADH oxidation. Genes from all three gene families encode proteins exclusively targeted to mitochondria, as revealed by expression of green fluorescent fusion proteins and by western blotting of fractionated cells. Phylogenetic analysis indicates that ndc1 affiliates with cyanobacterial type II NADH dehydrogenase genes, suggesting that this gene entered the eukaryotic cell via the chloroplast progenitor. The ndc1 should then have been transferred to the nucleus and acquired a signal for mitochondrial targeting of the protein product. Although they are of different origin, the nda, ndb, and ndc genes carry an identical intron position.},
  author       = {Michalecka, Agnieszka and Svensson, Staffan and Johansson, Fredrik I and Agius, Stephanie C and Johanson, Urban and Brennicke, Axel and Binder, Stefan and Rasmusson, Allan},
  issn         = {1532-2548},
  language     = {eng},
  number       = {2},
  pages        = {642--652},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Physiology},
  title        = {Arabidopsis genes encoding mitochondrial type II NAD(P)H dehydrogenases have different evolutionary orgin and show distinct responses to light.},
  url          = {http://dx.doi.org/},
  volume       = {133},
  year         = {2003},
}