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Optimisation of initial cell concentration enhances freeze-drying tolerance of Pseudomonas chlororaphis.

Palmfeldt, Johan LU ; Rådström, Peter LU and Hahn-Hägerdal, Bärbel LU (2003) In Cryobiology 47(1). p.21-29
Abstract
The freeze-drying tolerance of Pseudomonas chlororaphis, an antifungal bacterium used as biocontrol agent was investigated. P. chlororaphis is freeze-drying sensitive and the viability drops more than 3 log units in the absence of protective freeze-drying medium. Of the freeze-drying media tested, lactose, sucrose, trehalose, glutamate, sucrose with glutamate, skimmed milk, and skimmed milk with trehalose, skimmed milk gave the lowest survival (0.6 ± 0.2%) and sucrose the highest (6.4 ± 1.2%). Cellular accumulation of sucrose from the freeze-drying medium and the protective effect of sucrose were dependent on sucrose concentration. The effect of initial cell concentration, from 1 × 107 to 5 × 1010 CFU/ml, on survival after freeze-drying... (More)
The freeze-drying tolerance of Pseudomonas chlororaphis, an antifungal bacterium used as biocontrol agent was investigated. P. chlororaphis is freeze-drying sensitive and the viability drops more than 3 log units in the absence of protective freeze-drying medium. Of the freeze-drying media tested, lactose, sucrose, trehalose, glutamate, sucrose with glutamate, skimmed milk, and skimmed milk with trehalose, skimmed milk gave the lowest survival (0.6 ± 0.2%) and sucrose the highest (6.4 ± 1.2%). Cellular accumulation of sucrose from the freeze-drying medium and the protective effect of sucrose were dependent on sucrose concentration. The effect of initial cell concentration, from 1 × 107 to 5 × 1010 CFU/ml, on survival after freeze-drying was studied for carbon starved cells with sucrose as freeze-drying medium. The highest freeze-drying survival values, 15–25%, were obtained for initial cell concentrations between 1 × 109 and 1 × 1010 CFU/ml. For cell concentrations outside this window more than 10 times lower survival values were observed. P. chlororaphis was cultivated to induce stress response that could confer protection against freeze-drying inactivation. Carbon starvation and, to a lesser extent, heat treatment enhanced freeze-drying tolerance. By combining optimal cell concentration, optimal sucrose concentration and carbon starvation the survival after freeze-drying was 26 ± 6%. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Freeze-drying, Gram-negative bacteria, Sucrose, Cryoprotectant, Pseudomonas chlororaphis, Stress, Storage, Lyophilization
in
Cryobiology
volume
47
issue
1
pages
21 - 29
publisher
Elsevier
external identifiers
  • wos:000220794400003
  • pmid:12963409
  • scopus:0042831405
ISSN
0011-2240
DOI
10.1016/S0011-2240(03)00065-8
language
English
LU publication?
yes
id
5670d2e1-9c21-4004-9454-4dd7ecdf5e8e (old id 132828)
date added to LUP
2007-06-28 16:21:50
date last changed
2018-09-30 03:39:38
@article{5670d2e1-9c21-4004-9454-4dd7ecdf5e8e,
  abstract     = {The freeze-drying tolerance of Pseudomonas chlororaphis, an antifungal bacterium used as biocontrol agent was investigated. P. chlororaphis is freeze-drying sensitive and the viability drops more than 3 log units in the absence of protective freeze-drying medium. Of the freeze-drying media tested, lactose, sucrose, trehalose, glutamate, sucrose with glutamate, skimmed milk, and skimmed milk with trehalose, skimmed milk gave the lowest survival (0.6 ± 0.2%) and sucrose the highest (6.4 ± 1.2%). Cellular accumulation of sucrose from the freeze-drying medium and the protective effect of sucrose were dependent on sucrose concentration. The effect of initial cell concentration, from 1 × 107 to 5 × 1010 CFU/ml, on survival after freeze-drying was studied for carbon starved cells with sucrose as freeze-drying medium. The highest freeze-drying survival values, 15–25%, were obtained for initial cell concentrations between 1 × 109 and 1 × 1010 CFU/ml. For cell concentrations outside this window more than 10 times lower survival values were observed. P. chlororaphis was cultivated to induce stress response that could confer protection against freeze-drying inactivation. Carbon starvation and, to a lesser extent, heat treatment enhanced freeze-drying tolerance. By combining optimal cell concentration, optimal sucrose concentration and carbon starvation the survival after freeze-drying was 26 ± 6%.},
  author       = {Palmfeldt, Johan and Rådström, Peter and Hahn-Hägerdal, Bärbel},
  issn         = {0011-2240},
  keyword      = {Freeze-drying,Gram-negative bacteria,Sucrose,Cryoprotectant,Pseudomonas chlororaphis,Stress,Storage,Lyophilization},
  language     = {eng},
  number       = {1},
  pages        = {21--29},
  publisher    = {Elsevier},
  series       = {Cryobiology},
  title        = {Optimisation of initial cell concentration enhances freeze-drying tolerance of Pseudomonas chlororaphis.},
  url          = {http://dx.doi.org/10.1016/S0011-2240(03)00065-8},
  volume       = {47},
  year         = {2003},
}