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Graft repair of a peripheral nerve without the sacrifice of a healthy donor nerve by the use of acutely dissociated autologous Schwann cells.

Nilsson, Anna LU ; Dahlin, Lars LU ; Lundborg, Göran LU and Kanje, Martin LU (2005) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 39(1). p.1-6
Abstract
We hypothesised that pieces of nerve that are normally trimmed away during graft repair could be a source of Schwann cells and used to create an autologous cell-containing nerve graft. To test the idea a transsection injury was made on the rat sciatic nerve. Seven days later, pieces from the proximal and distal stumps were used for the immediate isolation of its non-neuronal cells. These cells, of which 80% were Schwann cells, were injected into a silicone tube bridging a 10 mm gap on the same nerve. Tubes containing cells showed superior regeneration of nerve fibres across the gap compared with tubes with no cells. This new repair technique is rapid, does not require the sacrifice of a healthy donor nerve, or the time-consuming culturing... (More)
We hypothesised that pieces of nerve that are normally trimmed away during graft repair could be a source of Schwann cells and used to create an autologous cell-containing nerve graft. To test the idea a transsection injury was made on the rat sciatic nerve. Seven days later, pieces from the proximal and distal stumps were used for the immediate isolation of its non-neuronal cells. These cells, of which 80% were Schwann cells, were injected into a silicone tube bridging a 10 mm gap on the same nerve. Tubes containing cells showed superior regeneration of nerve fibres across the gap compared with tubes with no cells. This new repair technique is rapid, does not require the sacrifice of a healthy donor nerve, or the time-consuming culturing and expansion of Schwann cells. The method has the potential to replace current methods for repair of extended nerve injuries. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Sciatic nerve, nerve repair, Schwann cell
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
volume
39
issue
1
pages
1 - 6
publisher
Taylor & Francis
external identifiers
  • wos:000227282500001
  • pmid:15848958
  • scopus:14644425251
ISSN
1651-2073
DOI
10.1080/02844310410017979
language
English
LU publication?
yes
id
ea16b829-d8e0-41bf-8239-daa291e582d4 (old id 136028)
date added to LUP
2007-07-23 12:43:04
date last changed
2017-02-12 04:11:32
@article{ea16b829-d8e0-41bf-8239-daa291e582d4,
  abstract     = {We hypothesised that pieces of nerve that are normally trimmed away during graft repair could be a source of Schwann cells and used to create an autologous cell-containing nerve graft. To test the idea a transsection injury was made on the rat sciatic nerve. Seven days later, pieces from the proximal and distal stumps were used for the immediate isolation of its non-neuronal cells. These cells, of which 80% were Schwann cells, were injected into a silicone tube bridging a 10 mm gap on the same nerve. Tubes containing cells showed superior regeneration of nerve fibres across the gap compared with tubes with no cells. This new repair technique is rapid, does not require the sacrifice of a healthy donor nerve, or the time-consuming culturing and expansion of Schwann cells. The method has the potential to replace current methods for repair of extended nerve injuries.},
  author       = {Nilsson, Anna and Dahlin, Lars and Lundborg, Göran and Kanje, Martin},
  issn         = {1651-2073},
  keyword      = {Sciatic nerve,nerve repair,Schwann cell},
  language     = {eng},
  number       = {1},
  pages        = {1--6},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery},
  title        = {Graft repair of a peripheral nerve without the sacrifice of a healthy donor nerve by the use of acutely dissociated autologous Schwann cells.},
  url          = {http://dx.doi.org/10.1080/02844310410017979},
  volume       = {39},
  year         = {2005},
}