Advanced

Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke

Carlen, Marie; Meletis, Konstantinos; Goritz, Christian; Darsalia, Vladimer LU ; Evergren, Emma; Tanigaki, Kenji; Amendola, Mario; Barnabe-Heider, Fanie; Yeung, Maggie S. Y. and Naldini, Luigi, et al. (2009) In Nature Neuroscience 12(3). p.259-267
Abstract
Neurons are continuously generated from stem cells in discrete regions in the adult mammalian brain. We found that ependymal cells lining the lateral ventricles were quiescent and did not contribute to adult neurogenesis under normal conditions in mice but instead gave rise to neuroblasts and astrocytes in response to stroke. Ependymal cell quiescence was actively maintained by canonical Notch signaling. Inhibition of this pathway in uninjured animals allowed ependymal cells to enter the cell cycle and produce olfactory bulb neurons, whereas forced Notch signaling was sufficient to block the ependymal cell response to stroke. Ependymal cells were depleted by stroke and failed to self-renew sufficiently to maintain their own population.... (More)
Neurons are continuously generated from stem cells in discrete regions in the adult mammalian brain. We found that ependymal cells lining the lateral ventricles were quiescent and did not contribute to adult neurogenesis under normal conditions in mice but instead gave rise to neuroblasts and astrocytes in response to stroke. Ependymal cell quiescence was actively maintained by canonical Notch signaling. Inhibition of this pathway in uninjured animals allowed ependymal cells to enter the cell cycle and produce olfactory bulb neurons, whereas forced Notch signaling was sufficient to block the ependymal cell response to stroke. Ependymal cells were depleted by stroke and failed to self-renew sufficiently to maintain their own population. Thus, although ependymal cells act as primary cells in the neural lineage to produce neurons and glial cells after stroke, they do not fulfill defining criteria for stem cells under these conditions and instead serve as a reservoir that is recruited by injury. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Neuroscience
volume
12
issue
3
pages
259 - 267
publisher
Nature Publishing Group
external identifiers
  • wos:000263577900010
  • scopus:60749099023
ISSN
1546-1726
DOI
10.1038/nn.2268
language
English
LU publication?
yes
id
4e73d8d8-aee6-4a90-ac16-4f2d90df0295 (old id 1372102)
date added to LUP
2009-05-08 14:07:48
date last changed
2017-12-17 03:41:39
@article{4e73d8d8-aee6-4a90-ac16-4f2d90df0295,
  abstract     = {Neurons are continuously generated from stem cells in discrete regions in the adult mammalian brain. We found that ependymal cells lining the lateral ventricles were quiescent and did not contribute to adult neurogenesis under normal conditions in mice but instead gave rise to neuroblasts and astrocytes in response to stroke. Ependymal cell quiescence was actively maintained by canonical Notch signaling. Inhibition of this pathway in uninjured animals allowed ependymal cells to enter the cell cycle and produce olfactory bulb neurons, whereas forced Notch signaling was sufficient to block the ependymal cell response to stroke. Ependymal cells were depleted by stroke and failed to self-renew sufficiently to maintain their own population. Thus, although ependymal cells act as primary cells in the neural lineage to produce neurons and glial cells after stroke, they do not fulfill defining criteria for stem cells under these conditions and instead serve as a reservoir that is recruited by injury.},
  author       = {Carlen, Marie and Meletis, Konstantinos and Goritz, Christian and Darsalia, Vladimer and Evergren, Emma and Tanigaki, Kenji and Amendola, Mario and Barnabe-Heider, Fanie and Yeung, Maggie S. Y. and Naldini, Luigi and Honjo, Tasuku and Kokaia, Zaal and Shupliakov, Oleg and Cassidy, Robert M. and Lindvall, Olle and Frisen, Jonas},
  issn         = {1546-1726},
  language     = {eng},
  number       = {3},
  pages        = {259--267},
  publisher    = {Nature Publishing Group},
  series       = {Nature Neuroscience},
  title        = {Forebrain ependymal cells are Notch-dependent and generate neuroblasts and astrocytes after stroke},
  url          = {http://dx.doi.org/10.1038/nn.2268},
  volume       = {12},
  year         = {2009},
}