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Early changes in gene expression in the dorsal root ganglia after transection of the sciatic nerve; effects of amphiregulin and PAI-1 on regeneration.

Nilsson, Anna LU ; Moller, Kristian LU ; Dahlin, Lars LU ; Lundborg, Göran LU and Kanje, Martin LU (2005) In Brain Research. Molecular Brain Research 136(1-2). p.65-74
Abstract
To characterize the gene activity that may be required for neuronal survival and regeneration, we used the Affymetrix GeneChip Mu74A to screen 12000 genes and expressed sequence tag (EST) mRNA from L4 and L5 mouse dorsal root ganglia (DRG) 12 h and 24 h after sciatic nerve transection. At 12 h, we found 17 upregulated transcripts, and at 24 h, 49 that met our criteria of a significant 2-fold increase in expression. The alterations included a total of eight transcription factors and several genes associated with TGF-beta- and IL-6-mediated signaling. Two of the changes, amphiregulin and plasminogen activator inhibitor-1 (PAI-1), were confirmed by real-time quantitative PCR (QPCR). Addition of amphiregulin (20 ng/ml) to organ-cultured DRG... (More)
To characterize the gene activity that may be required for neuronal survival and regeneration, we used the Affymetrix GeneChip Mu74A to screen 12000 genes and expressed sequence tag (EST) mRNA from L4 and L5 mouse dorsal root ganglia (DRG) 12 h and 24 h after sciatic nerve transection. At 12 h, we found 17 upregulated transcripts, and at 24 h, 49 that met our criteria of a significant 2-fold increase in expression. The alterations included a total of eight transcription factors and several genes associated with TGF-beta- and IL-6-mediated signaling. Two of the changes, amphiregulin and plasminogen activator inhibitor-1 (PAI-1), were confirmed by real-time quantitative PCR (QPCR). Addition of amphiregulin (20 ng/ml) to organ-cultured DRG stimulated axonal outgrowth while PAI-1 (20 nM) inhibited migration of Schwann cells from the ganglia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dorsal root ganglia, Gene, Micro-array, Mouse, Nerve regeneration
in
Brain Research. Molecular Brain Research
volume
136
issue
1-2
pages
65 - 74
publisher
Elsevier
external identifiers
  • pmid:15893588
  • wos:000229497400008
  • scopus:19044374577
ISSN
0169-328X
DOI
10.1016/j.molbrainres.2005.01.008
language
English
LU publication?
yes
id
1c10c016-1bd5-426d-9978-ffe8ec9fd84b (old id 138014)
date added to LUP
2007-07-23 12:41:27
date last changed
2017-01-01 06:37:46
@article{1c10c016-1bd5-426d-9978-ffe8ec9fd84b,
  abstract     = {To characterize the gene activity that may be required for neuronal survival and regeneration, we used the Affymetrix GeneChip Mu74A to screen 12000 genes and expressed sequence tag (EST) mRNA from L4 and L5 mouse dorsal root ganglia (DRG) 12 h and 24 h after sciatic nerve transection. At 12 h, we found 17 upregulated transcripts, and at 24 h, 49 that met our criteria of a significant 2-fold increase in expression. The alterations included a total of eight transcription factors and several genes associated with TGF-beta- and IL-6-mediated signaling. Two of the changes, amphiregulin and plasminogen activator inhibitor-1 (PAI-1), were confirmed by real-time quantitative PCR (QPCR). Addition of amphiregulin (20 ng/ml) to organ-cultured DRG stimulated axonal outgrowth while PAI-1 (20 nM) inhibited migration of Schwann cells from the ganglia.},
  author       = {Nilsson, Anna and Moller, Kristian and Dahlin, Lars and Lundborg, Göran and Kanje, Martin},
  issn         = {0169-328X},
  keyword      = {Dorsal root ganglia,Gene,Micro-array,Mouse,Nerve regeneration},
  language     = {eng},
  number       = {1-2},
  pages        = {65--74},
  publisher    = {Elsevier},
  series       = {Brain Research. Molecular Brain Research},
  title        = {Early changes in gene expression in the dorsal root ganglia after transection of the sciatic nerve; effects of amphiregulin and PAI-1 on regeneration.},
  url          = {http://dx.doi.org/10.1016/j.molbrainres.2005.01.008},
  volume       = {136},
  year         = {2005},
}