Advanced

Neural stem and progenitor cells retain their potential for proliferation and differentiation into functional neurons despite lower number in aged brain.

Ahlenius, Henrik LU ; Visan, Violeta LU ; Kokaia, Merab LU ; Lindvall, Olle LU and Kokaia, Zaal LU (2009) In The Journal of neuroscience : the official journal of the Society for Neuroscience 29(14). p.4408-4419
Abstract
Neurogenesis in the subventricular zone (SVZ), which gives rise to new neurons in the olfactory bulb, continues throughout life but declines with increasing age. Little is known about how aging affects the intrinsic properties of the neural stem and progenitor cells (NSCs) in SVZ and the functional characteristics of their neuronal progeny. Here, we have compared the properties of NSCs isolated from embryonic lateral ganglionic eminence and adult and aged SVZ in mice using in vivo and in vitro systems, analyzed their gene expression profile, and studied their electrophysiological characteristics before and after differentiation into neurons. We show a loss of NSCs in SVZ from aged mice accompanied by reduced expression of genes for NSC... (More)
Neurogenesis in the subventricular zone (SVZ), which gives rise to new neurons in the olfactory bulb, continues throughout life but declines with increasing age. Little is known about how aging affects the intrinsic properties of the neural stem and progenitor cells (NSCs) in SVZ and the functional characteristics of their neuronal progeny. Here, we have compared the properties of NSCs isolated from embryonic lateral ganglionic eminence and adult and aged SVZ in mice using in vivo and in vitro systems, analyzed their gene expression profile, and studied their electrophysiological characteristics before and after differentiation into neurons. We show a loss of NSCs in SVZ from aged mice accompanied by reduced expression of genes for NSC markers, developmentally important transcription factors, and neurogenic factors. However, when isolated in vitro, the NSCs from SVZ of aged animals have capacity for proliferation and multilineage differentiation, including production of functional neurons, similar to that of NSCs in adult mice, albeit with lower efficacy. These properties are of major importance when considering therapeutic applications of neuronal replacement from endogenous NSCs in the injured, aged brain. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of neuroscience : the official journal of the Society for Neuroscience
volume
29
issue
14
pages
4408 - 4419
publisher
Society for Neuroscience
external identifiers
  • wos:000265009600009
  • pmid:19357268
  • scopus:65549125564
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.6003-08.2009
language
English
LU publication?
yes
id
e73dc994-458a-43e0-a7b1-a3003e42476b (old id 1392241)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19357268?dopt=Abstract
date added to LUP
2009-05-06 14:22:23
date last changed
2017-04-02 04:17:01
@article{e73dc994-458a-43e0-a7b1-a3003e42476b,
  abstract     = {Neurogenesis in the subventricular zone (SVZ), which gives rise to new neurons in the olfactory bulb, continues throughout life but declines with increasing age. Little is known about how aging affects the intrinsic properties of the neural stem and progenitor cells (NSCs) in SVZ and the functional characteristics of their neuronal progeny. Here, we have compared the properties of NSCs isolated from embryonic lateral ganglionic eminence and adult and aged SVZ in mice using in vivo and in vitro systems, analyzed their gene expression profile, and studied their electrophysiological characteristics before and after differentiation into neurons. We show a loss of NSCs in SVZ from aged mice accompanied by reduced expression of genes for NSC markers, developmentally important transcription factors, and neurogenic factors. However, when isolated in vitro, the NSCs from SVZ of aged animals have capacity for proliferation and multilineage differentiation, including production of functional neurons, similar to that of NSCs in adult mice, albeit with lower efficacy. These properties are of major importance when considering therapeutic applications of neuronal replacement from endogenous NSCs in the injured, aged brain.},
  author       = {Ahlenius, Henrik and Visan, Violeta and Kokaia, Merab and Lindvall, Olle and Kokaia, Zaal},
  issn         = {1529-2401},
  language     = {eng},
  number       = {14},
  pages        = {4408--4419},
  publisher    = {Society for Neuroscience},
  series       = {The Journal of neuroscience : the official journal of the Society for Neuroscience},
  title        = {Neural stem and progenitor cells retain their potential for proliferation and differentiation into functional neurons despite lower number in aged brain.},
  url          = {http://dx.doi.org/10.1523/JNEUROSCI.6003-08.2009},
  volume       = {29},
  year         = {2009},
}