Axonal outgrowth in muscle grafts made acellular by chemical extraction
Arai, Takeru ; Kanje, Martin LU ; Lundborg, Göran LU ; Sondell, Mariann ; Liu, Xiao Lin and Dahlin, Lars B. LU
(2000)
In Restorative Neurology and Neuroscience
17(4).
p.165-174
- Abstract
Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of... (More)
Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of basal laminae of the muscle fibers. The freeze/thawed grafts were brittle and lacked elasticity, but resulted in minor changes in major proteins. The axons regenerated through both types of grafts (initial delay 6 days and rate 0.7-0.8 mm/day), which shrunk in length by 25 %. There were no apparent differences with respect to Schwann cells and macrophages. Conclusions: The results suggest that detergent extracted mucle tissue, in which some basal lamina proteins remain but cells are removed, could present a new favourable option for nerve grafting.
(Less)
- author
- Arai, Takeru
; Kanje, Martin
LU
; Lundborg, Göran
LU
; Sondell, Mariann
; Liu, Xiao Lin
and Dahlin, Lars B.
LU
- organization
- publishing date
- 2000-12
- type
- Contribution to journal
- publication status
- published
- keywords
- Axons, Extraction, Macrophages, Muscle, Nerve grafts, Nerve regeneration, Schwann cells
- in
- Restorative Neurology and Neuroscience
- volume
- 17
- issue
- 4
- pages
- 165 - 174
- publisher
- IOS Press
- external identifiers
-
- scopus:0034437578
- ISSN
- 0922-6028
- language
- English
- LU publication?
- yes
- id
- 13c31f1f-67bd-4a2e-b8ff-0fc90e754895
- alternative location
- https://content.iospress.com/articles/restorative-neurology-and-neuroscience/rnn00154
- date added to LUP
- 2019-06-17 14:04:33
- date last changed
- 2025-04-04 01:17:09
@article{13c31f1f-67bd-4a2e-b8ff-0fc90e754895,
abstract = {{<p>Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of basal laminae of the muscle fibers. The freeze/thawed grafts were brittle and lacked elasticity, but resulted in minor changes in major proteins. The axons regenerated through both types of grafts (initial delay 6 days and rate 0.7-0.8 mm/day), which shrunk in length by 25 %. There were no apparent differences with respect to Schwann cells and macrophages. Conclusions: The results suggest that detergent extracted mucle tissue, in which some basal lamina proteins remain but cells are removed, could present a new favourable option for nerve grafting.</p>}},
author = {{Arai, Takeru and Kanje, Martin and Lundborg, Göran and Sondell, Mariann and Liu, Xiao Lin and Dahlin, Lars B.}},
issn = {{0922-6028}},
keywords = {{Axons; Extraction; Macrophages; Muscle; Nerve grafts; Nerve regeneration; Schwann cells}},
language = {{eng}},
number = {{4}},
pages = {{165--174}},
publisher = {{IOS Press}},
series = {{Restorative Neurology and Neuroscience}},
title = {{Axonal outgrowth in muscle grafts made acellular by chemical extraction}},
url = {{https://content.iospress.com/articles/restorative-neurology-and-neuroscience/rnn00154}},
volume = {{17}},
year = {{2000}},
}