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Axonal outgrowth in muscle grafts made acellular by chemical extraction

Arai, Takeru ; Kanje, Martin LU ; Lundborg, Göran LU ; Sondell, Mariann ; Liu, Xiao Lin and Dahlin, Lars B. LU (2000) In Restorative Neurology and Neuroscience 17(4). p.165-174
Abstract

Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of... (More)

Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of basal laminae of the muscle fibers. The freeze/thawed grafts were brittle and lacked elasticity, but resulted in minor changes in major proteins. The axons regenerated through both types of grafts (initial delay 6 days and rate 0.7-0.8 mm/day), which shrunk in length by 25 %. There were no apparent differences with respect to Schwann cells and macrophages. Conclusions: The results suggest that detergent extracted mucle tissue, in which some basal lamina proteins remain but cells are removed, could present a new favourable option for nerve grafting.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axons, Extraction, Macrophages, Muscle, Nerve grafts, Nerve regeneration, Schwann cells
in
Restorative Neurology and Neuroscience
volume
17
issue
4
pages
165 - 174
publisher
IOS Press
external identifiers
  • scopus:0034437578
ISSN
0922-6028
language
English
LU publication?
yes
id
13c31f1f-67bd-4a2e-b8ff-0fc90e754895
alternative location
https://content.iospress.com/articles/restorative-neurology-and-neuroscience/rnn00154
date added to LUP
2019-06-17 14:04:33
date last changed
2020-06-16 12:53:22
@article{13c31f1f-67bd-4a2e-b8ff-0fc90e754895,
  abstract     = {<p>Purpose: To compare nerve regeneration in autologous detergent extracted and freeze-thawed muscle grafts and to electrophoretically characterize the grafts. Methods: Autologous acellular muscle grafts were created either by freeze/thawing or by detergent extraction and then used to bridge a 10 mm gap in rat sciatic nerve. The autologous grafts were compared with respect to protein content, using electrophoresis preimplantation, and axonal outgrowth, Schwann cell and macrophage content, using immunocytochemistry (neurofilaments, S-100 protein, ED 1 macrophages) at 5-20 days postimplantation. Results: The extracted muscle grafts were elastic, but the amount of several proteins was reduced and laminin was still present at a position of basal laminae of the muscle fibers. The freeze/thawed grafts were brittle and lacked elasticity, but resulted in minor changes in major proteins. The axons regenerated through both types of grafts (initial delay 6 days and rate 0.7-0.8 mm/day), which shrunk in length by 25 %. There were no apparent differences with respect to Schwann cells and macrophages. Conclusions: The results suggest that detergent extracted mucle tissue, in which some basal lamina proteins remain but cells are removed, could present a new favourable option for nerve grafting.</p>},
  author       = {Arai, Takeru and Kanje, Martin and Lundborg, Göran and Sondell, Mariann and Liu, Xiao Lin and Dahlin, Lars B.},
  issn         = {0922-6028},
  language     = {eng},
  number       = {4},
  pages        = {165--174},
  publisher    = {IOS Press},
  series       = {Restorative Neurology and Neuroscience},
  title        = {Axonal outgrowth in muscle grafts made acellular by chemical extraction},
  url          = {https://content.iospress.com/articles/restorative-neurology-and-neuroscience/rnn00154},
  volume       = {17},
  year         = {2000},
}