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Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

Gomez, Federico LU ; Dejmek, Petr LU ; Lundgren, Krister; Rasmusson, Allan LU ; Vicente, Antonio and Moritz, Thomas (2009) In Planta 230(3). p.469-479
Abstract
Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in... (More)
Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Electroporation, Cell membrane recovery, Metabolic analysis, multivariate data analysis, OPLS, Wounding, Stress response
in
Planta
volume
230
issue
3
pages
469 - 479
publisher
Springer
external identifiers
  • wos:000268393600003
  • scopus:67749099313
ISSN
0032-0935
DOI
10.1007/s00425-009-0950-2
language
English
LU publication?
yes
id
2ee1792e-5181-4a06-9f74-d90abe12ce77 (old id 1460415)
date added to LUP
2009-08-24 16:09:32
date last changed
2017-10-08 04:00:53
@article{2ee1792e-5181-4a06-9f74-d90abe12ce77,
  abstract     = {Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.},
  author       = {Gomez, Federico and Dejmek, Petr and Lundgren, Krister and Rasmusson, Allan and Vicente, Antonio and Moritz, Thomas},
  issn         = {0032-0935},
  keyword      = {Electroporation,Cell membrane recovery,Metabolic analysis,multivariate data analysis,OPLS,Wounding,Stress response},
  language     = {eng},
  number       = {3},
  pages        = {469--479},
  publisher    = {Springer},
  series       = {Planta},
  title        = {Metabolomic evaluation of pulsed electric field-induced stress on potato tissue},
  url          = {http://dx.doi.org/10.1007/s00425-009-0950-2},
  volume       = {230},
  year         = {2009},
}