Metabolomic evaluation of pulsed electric field-induced stress on potato tissue
Gomez, Federico LU ; Dejmek, Petr LU
; Lundgren, Krister
; Rasmusson, Allan
LU
; Vicente, Antonio
and Moritz, Thomas
(2009)
In Planta
230(3).
p.469-479
- Abstract
- Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in... (More)
- Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1460415
- author
- Gomez, Federico
LU
; Dejmek, Petr
LU
; Lundgren, Krister
; Rasmusson, Allan
LU
; Vicente, Antonio
and Moritz, Thomas
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Electroporation, Cell membrane recovery, Metabolic analysis, multivariate data analysis, OPLS, Wounding, Stress response
- in
- Planta
- volume
- 230
- issue
- 3
- pages
- 469 - 479
- publisher
- Springer
- external identifiers
-
- wos:000268393600003
- scopus:67749099313
- pmid:19495789
- ISSN
- 0032-0935
- DOI
- 10.1007/s00425-009-0950-2
- language
- English
- LU publication?
- yes
- id
- 2ee1792e-5181-4a06-9f74-d90abe12ce77 (old id 1460415)
- date added to LUP
- 2016-04-01 14:18:14
- date last changed
- 2024-04-24 21:58:28
@article{2ee1792e-5181-4a06-9f74-d90abe12ce77,
abstract = {{Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.}},
author = {{Gomez, Federico and Dejmek, Petr and Lundgren, Krister and Rasmusson, Allan and Vicente, Antonio and Moritz, Thomas}},
issn = {{0032-0935}},
keywords = {{Electroporation; Cell membrane recovery; Metabolic analysis; multivariate data analysis; OPLS; Wounding; Stress response}},
language = {{eng}},
number = {{3}},
pages = {{469--479}},
publisher = {{Springer}},
series = {{Planta}},
title = {{Metabolomic evaluation of pulsed electric field-induced stress on potato tissue}},
url = {{http://dx.doi.org/10.1007/s00425-009-0950-2}},
doi = {{10.1007/s00425-009-0950-2}},
volume = {{230}},
year = {{2009}},
}