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Bacterial growth on photochemically transformed leachates from aquatic and terrestrial primary producers

Anesio, Alexandre Magno LU ; Theil-Nielsen, J and Granéli, Wilhelm LU (2000) In Microbial Ecology 40(3). p.200-208
Abstract
We measured bacterial growth on phototransformed dissolved organic matter (DOM) leached from eight different primary producers. Leachates (10 mg C liter(-1)) were exposed to artificial UVA + UVB radiation, or kept in darkness, for 20 h. DOM solutions were subsequently inoculated with lake water bacteria. Photoproduction of dissolved inorganic carbon (DIC), ranging from 3 to 16 mug C liter(-1) h(-1), and changes in the absorptive characteristics of the DOM were observed for all leachates upon UV irradiation. The effects of irradiation exposure on DOM bioavailability varied greatly, depending on leachate and type of bacterial growth criterion. Bacterial carbon utilization (biomass production plus respiration) over the entire incubation... (More)
We measured bacterial growth on phototransformed dissolved organic matter (DOM) leached from eight different primary producers. Leachates (10 mg C liter(-1)) were exposed to artificial UVA + UVB radiation, or kept in darkness, for 20 h. DOM solutions were subsequently inoculated with lake water bacteria. Photoproduction of dissolved inorganic carbon (DIC), ranging from 3 to 16 mug C liter(-1) h(-1), and changes in the absorptive characteristics of the DOM were observed for all leachates upon UV irradiation. The effects of irradiation exposure on DOM bioavailability varied greatly, depending on leachate and type of bacterial growth criterion. Bacterial carbon utilization (biomass production plus respiration) over the entire incubation period (120 h) was enhanced by UV radiation of leachate from the terrestrial leaves, relative to carbon utilization in non-irradiated leachates. Conversely, carbon utilization was reduced by radiation of the leachates from aquatic macrophytes. In a separate experiment, the stable C and N isotope composition of bacteria grown on irradiated and non-irradiated DOM was estimated. Bacterial growth on UV-irradiated DOM was enriched in C-13 relative to the bacteria in the non-irradiated treatments; this result may be explained by selective assimilation of photochemically produced, isotopically enriched labile compounds. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Ecology
volume
40
issue
3
pages
200 - 208
publisher
Springer
external identifiers
  • scopus:0033650594
ISSN
1432-184X
DOI
10.1007/s002480000045
language
English
LU publication?
yes
id
b468c3f8-c3b2-4983-99e4-361eed636572 (old id 146703)
date added to LUP
2007-06-28 12:02:53
date last changed
2017-01-01 04:31:03
@article{b468c3f8-c3b2-4983-99e4-361eed636572,
  abstract     = {We measured bacterial growth on phototransformed dissolved organic matter (DOM) leached from eight different primary producers. Leachates (10 mg C liter(-1)) were exposed to artificial UVA + UVB radiation, or kept in darkness, for 20 h. DOM solutions were subsequently inoculated with lake water bacteria. Photoproduction of dissolved inorganic carbon (DIC), ranging from 3 to 16 mug C liter(-1) h(-1), and changes in the absorptive characteristics of the DOM were observed for all leachates upon UV irradiation. The effects of irradiation exposure on DOM bioavailability varied greatly, depending on leachate and type of bacterial growth criterion. Bacterial carbon utilization (biomass production plus respiration) over the entire incubation period (120 h) was enhanced by UV radiation of leachate from the terrestrial leaves, relative to carbon utilization in non-irradiated leachates. Conversely, carbon utilization was reduced by radiation of the leachates from aquatic macrophytes. In a separate experiment, the stable C and N isotope composition of bacteria grown on irradiated and non-irradiated DOM was estimated. Bacterial growth on UV-irradiated DOM was enriched in C-13 relative to the bacteria in the non-irradiated treatments; this result may be explained by selective assimilation of photochemically produced, isotopically enriched labile compounds.},
  author       = {Anesio, Alexandre Magno and Theil-Nielsen, J and Granéli, Wilhelm},
  issn         = {1432-184X},
  language     = {eng},
  number       = {3},
  pages        = {200--208},
  publisher    = {Springer},
  series       = {Microbial Ecology},
  title        = {Bacterial growth on photochemically transformed leachates from aquatic and terrestrial primary producers},
  url          = {http://dx.doi.org/10.1007/s002480000045},
  volume       = {40},
  year         = {2000},
}