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Methylhexahydrophthalic anhydride adducted albumin tryptic peptides in nasal lavage fluid.

Jeppsson, Marina LU ; Lindh, Christian LU ; Kåredal, Monica LU ; Nielsen, Jörn LU and Jönsson, Bo A LU (2009) In Inhalation Toxicology 21(12). p.1013-1020
Abstract
Methylhexahydrophthalic anhydride (MHHPA) is a reactive, low molecular weight chemical used in products such as plastics, paints, and electronic components. Exposure to MHHPA may lead to work-related airway diseases such as rhinitis, conjunctivitis, and asthma. Twelve subjects employed at a plant manufacturing electrical capacitors using MHHPA were included in this study. Nasal lavages were collected from subjects before work Monday morning and after work Tuesday afternoon. The levels of MHHPA adducted to serum albumin were analyzed with a straightforward work-up method. The samples were trypsinated before being analyzed with a liquid chromatography-triple quadrupole mass spectrometer. The mass spectrometer was run using selected reaction... (More)
Methylhexahydrophthalic anhydride (MHHPA) is a reactive, low molecular weight chemical used in products such as plastics, paints, and electronic components. Exposure to MHHPA may lead to work-related airway diseases such as rhinitis, conjunctivitis, and asthma. Twelve subjects employed at a plant manufacturing electrical capacitors using MHHPA were included in this study. Nasal lavages were collected from subjects before work Monday morning and after work Tuesday afternoon. The levels of MHHPA adducted to serum albumin were analyzed with a straightforward work-up method. The samples were trypsinated before being analyzed with a liquid chromatography-triple quadrupole mass spectrometer. The mass spectrometer was run using selected reaction monitoring for six adducted peptides. Also, some biomarkers of effect (albumin, total protein, eosinophil cationic protein, and tryptase) were analyzed in nasal lavages. Furthermore, the metabolite MHHP acid in urine after work on Tuesday was analyzed by gas chromatography-mass spectrometry. Symptoms from the airways and the eyes and sensitization were registered. The main result of this study is that protein adducts can be analyzed in vivo after low occupational exposures to MHHPA. The results also show a correlation between adducted peptides and albumin in nasal lavage. Furthermore, there may be a difference in the potential to induce hyperresponsiveness between adducts bound to different amino acids. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Inhalation Toxicology
volume
21
issue
12
pages
1013 - 1020
publisher
Taylor & Francis
external identifiers
  • wos:000273619200006
  • pmid:19772480
  • scopus:70350664450
ISSN
0895-8378
DOI
10.1080/08958370802715997
language
English
LU publication?
yes
id
b2c9ac88-31dd-488e-8967-289ef7d6d384 (old id 1483249)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19772480?dopt=Abstract
date added to LUP
2012-01-18 13:59:31
date last changed
2017-11-19 03:57:56
@article{b2c9ac88-31dd-488e-8967-289ef7d6d384,
  abstract     = {Methylhexahydrophthalic anhydride (MHHPA) is a reactive, low molecular weight chemical used in products such as plastics, paints, and electronic components. Exposure to MHHPA may lead to work-related airway diseases such as rhinitis, conjunctivitis, and asthma. Twelve subjects employed at a plant manufacturing electrical capacitors using MHHPA were included in this study. Nasal lavages were collected from subjects before work Monday morning and after work Tuesday afternoon. The levels of MHHPA adducted to serum albumin were analyzed with a straightforward work-up method. The samples were trypsinated before being analyzed with a liquid chromatography-triple quadrupole mass spectrometer. The mass spectrometer was run using selected reaction monitoring for six adducted peptides. Also, some biomarkers of effect (albumin, total protein, eosinophil cationic protein, and tryptase) were analyzed in nasal lavages. Furthermore, the metabolite MHHP acid in urine after work on Tuesday was analyzed by gas chromatography-mass spectrometry. Symptoms from the airways and the eyes and sensitization were registered. The main result of this study is that protein adducts can be analyzed in vivo after low occupational exposures to MHHPA. The results also show a correlation between adducted peptides and albumin in nasal lavage. Furthermore, there may be a difference in the potential to induce hyperresponsiveness between adducts bound to different amino acids.},
  author       = {Jeppsson, Marina and Lindh, Christian and Kåredal, Monica and Nielsen, Jörn and Jönsson, Bo A},
  issn         = {0895-8378},
  language     = {eng},
  number       = {12},
  pages        = {1013--1020},
  publisher    = {Taylor & Francis},
  series       = {Inhalation Toxicology},
  title        = {Methylhexahydrophthalic anhydride adducted albumin tryptic peptides in nasal lavage fluid.},
  url          = {http://dx.doi.org/10.1080/08958370802715997},
  volume       = {21},
  year         = {2009},
}