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One of the two genes encoding nucleoid-associated HU proteins in Streptomyces coelicolor is developmentally regulated and specifically involved in spore maturation.

Salerno, Paola LU ; Larsson, Jessica LU ; Bucca, Giselda; Laing, Emma; Smith, Colin P and Flärdh, Klas LU (2009) In Journal of Bacteriology 191. p.6489-6500
Abstract
Streptomyces genomes encode two homologs of the nucleoid-associated HU-proteins. One of them, here designated HupA, is of a conventional type similar to E. coli HUalpha and HUbeta, while the other, HupS, is a two-domain protein. In addition to the N-terminal part that is similar to HU proteins, it has an additional C-terminal domain that is similar to the alanine and lysine-rich C-termini of eukaryotic linker histones. Such two-domain HU proteins are only found among Actinobacteria. In this phylum some organisms have only a single HU-protein of this type with a C-terminal histone H1-like domain (e. g. Hlp in Mycobacterium smegmatis), while others have only a single conventional HU. Yet others, including the streptomycetes, produce both... (More)
Streptomyces genomes encode two homologs of the nucleoid-associated HU-proteins. One of them, here designated HupA, is of a conventional type similar to E. coli HUalpha and HUbeta, while the other, HupS, is a two-domain protein. In addition to the N-terminal part that is similar to HU proteins, it has an additional C-terminal domain that is similar to the alanine and lysine-rich C-termini of eukaryotic linker histones. Such two-domain HU proteins are only found among Actinobacteria. In this phylum some organisms have only a single HU-protein of this type with a C-terminal histone H1-like domain (e. g. Hlp in Mycobacterium smegmatis), while others have only a single conventional HU. Yet others, including the streptomycetes, produce both types of HU-proteins. We show here that the two HU-genes in Streptomyces coelicolor are differentially regulated and that hupS is specifically expressed during sporulation, while hupA is expressed in vegetative hyphae. The developmental upregulation of hupS occurred in sporogenic aerial hyphal compartments, and was dependent on the developmental regulators whiA, whiG, and whiI. HupS was found to be nucleoid-associated in spores, and a hupS deletion mutant had larger average nucleoid size in spores compared to the parent strain. The mutant spores were also defective in heat resistance and spore pigmentation, although they showed apparently normal spore walls and displayed no increased sensitivity to detergents. Overall, the results show that HupS is specifically involved in sporulation and may affect nucleoid architecture and protection in spores of S. coelicolor. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
191
pages
6489 - 6500
publisher
American Society for Microbiology
external identifiers
  • wos:000270723700003
  • scopus:70350462621
ISSN
0021-9193
DOI
10.1128/JB.00709-09
language
English
LU publication?
yes
id
94067ca9-4e46-4da8-9514-21e68a4b729b (old id 1484004)
date added to LUP
2009-10-08 12:16:14
date last changed
2017-12-10 03:51:16
@article{94067ca9-4e46-4da8-9514-21e68a4b729b,
  abstract     = {Streptomyces genomes encode two homologs of the nucleoid-associated HU-proteins. One of them, here designated HupA, is of a conventional type similar to E. coli HUalpha and HUbeta, while the other, HupS, is a two-domain protein. In addition to the N-terminal part that is similar to HU proteins, it has an additional C-terminal domain that is similar to the alanine and lysine-rich C-termini of eukaryotic linker histones. Such two-domain HU proteins are only found among Actinobacteria. In this phylum some organisms have only a single HU-protein of this type with a C-terminal histone H1-like domain (e. g. Hlp in Mycobacterium smegmatis), while others have only a single conventional HU. Yet others, including the streptomycetes, produce both types of HU-proteins. We show here that the two HU-genes in Streptomyces coelicolor are differentially regulated and that hupS is specifically expressed during sporulation, while hupA is expressed in vegetative hyphae. The developmental upregulation of hupS occurred in sporogenic aerial hyphal compartments, and was dependent on the developmental regulators whiA, whiG, and whiI. HupS was found to be nucleoid-associated in spores, and a hupS deletion mutant had larger average nucleoid size in spores compared to the parent strain. The mutant spores were also defective in heat resistance and spore pigmentation, although they showed apparently normal spore walls and displayed no increased sensitivity to detergents. Overall, the results show that HupS is specifically involved in sporulation and may affect nucleoid architecture and protection in spores of S. coelicolor.},
  author       = {Salerno, Paola and Larsson, Jessica and Bucca, Giselda and Laing, Emma and Smith, Colin P and Flärdh, Klas},
  issn         = {0021-9193},
  language     = {eng},
  pages        = {6489--6500},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {One of the two genes encoding nucleoid-associated HU proteins in Streptomyces coelicolor is developmentally regulated and specifically involved in spore maturation.},
  url          = {http://dx.doi.org/10.1128/JB.00709-09},
  volume       = {191},
  year         = {2009},
}