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Proteomic Profiling of Acoustically Isolated Extracellular Vesicles from Blood Plasma during Murine Bacterial Sepsis

Broman, Axel LU ; Chao, Yashuan LU ; Shannon, Oonagh LU ; Laurell, Thomas LU and Malmström, Johan LU orcid (2025) In Journal of Proteome Research
Abstract

Sepsis is a life-threatening condition caused by a dysregulated host response to an infection and is a leading cause of death worldwide. The condition is variable, which in combination with insufficient clinical markers, makes it challenging to predict when infection will progress to sepsis and to categorize patients into homogeneous patient subgroups. In this study, we demonstrate the use of acoustic trapping to rapidly enrich extracellular vesicles (EVs) from minute volumes of blood plasma from experimental mouse models of sepsis infected with the Gram-positive pathogen
Streptococcus pyogenes or the Gram-negative pathogen
Escherichia coli. Using quantitative mass spectrometry-based proteomics, we characterized the proteome... (More)

Sepsis is a life-threatening condition caused by a dysregulated host response to an infection and is a leading cause of death worldwide. The condition is variable, which in combination with insufficient clinical markers, makes it challenging to predict when infection will progress to sepsis and to categorize patients into homogeneous patient subgroups. In this study, we demonstrate the use of acoustic trapping to rapidly enrich extracellular vesicles (EVs) from minute volumes of blood plasma from experimental mouse models of sepsis infected with the Gram-positive pathogen
Streptococcus pyogenes or the Gram-negative pathogen
Escherichia coli. Using quantitative mass spectrometry-based proteomics, we characterized the proteome of EVs and plasma to demonstrate that the EVs expand the observable proteome in plasma, with an emphasis on cellular processes and signaling. In our models, systemic bacterial infection altered the EV and plasma proteomes differently, with a predominant effect on proteins related to leukocyte migration in the EVs and on metabolism in the plasma. Finally, we show that
E. coli infection significantly impacted metabolism, whereas
S. pyogenes infection mainly affected the inflammatory response and neutrophil degranulation in our models. Collectively, our findings demonstrate that the acoustic trap facilitates access to plasma EVs, which in turn provides additional biological information which was not obtained from the plasma proteome alone.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
in
Journal of Proteome Research
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:105010315415
  • pmid:40641435
ISSN
1535-3893
DOI
10.1021/acs.jproteome.5c00267
language
English
LU publication?
yes
id
14f55d96-f148-4020-a51f-2e7dfd3bcba0
date added to LUP
2025-07-31 09:19:27
date last changed
2025-08-01 04:03:00
@article{14f55d96-f148-4020-a51f-2e7dfd3bcba0,
  abstract     = {{<p>Sepsis is a life-threatening condition caused by a dysregulated host response to an infection and is a leading cause of death worldwide. The condition is variable, which in combination with insufficient clinical markers, makes it challenging to predict when infection will progress to sepsis and to categorize patients into homogeneous patient subgroups. In this study, we demonstrate the use of acoustic trapping to rapidly enrich extracellular vesicles (EVs) from minute volumes of blood plasma from experimental mouse models of sepsis infected with the Gram-positive pathogen <br>
 Streptococcus pyogenes or the Gram-negative pathogen <br>
 Escherichia coli. Using quantitative mass spectrometry-based proteomics, we characterized the proteome of EVs and plasma to demonstrate that the EVs expand the observable proteome in plasma, with an emphasis on cellular processes and signaling. In our models, systemic bacterial infection altered the EV and plasma proteomes differently, with a predominant effect on proteins related to leukocyte migration in the EVs and on metabolism in the plasma. Finally, we show that<br>
 E. coli infection significantly impacted metabolism, whereas <br>
 S. pyogenes infection mainly affected the inflammatory response and neutrophil degranulation in our models. Collectively, our findings demonstrate that the acoustic trap facilitates access to plasma EVs, which in turn provides additional biological information which was not obtained from the plasma proteome alone.<br>
 </p>}},
  author       = {{Broman, Axel and Chao, Yashuan and Shannon, Oonagh and Laurell, Thomas and Malmström, Johan}},
  issn         = {{1535-3893}},
  language     = {{eng}},
  month        = {{07}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{Proteomic Profiling of Acoustically Isolated Extracellular Vesicles from Blood Plasma during Murine Bacterial Sepsis}},
  url          = {{http://dx.doi.org/10.1021/acs.jproteome.5c00267}},
  doi          = {{10.1021/acs.jproteome.5c00267}},
  year         = {{2025}},
}