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Omics Analyses Reveal a Potential Link between Hormone-Sensitive Lipase and Polyamine Metabolism.

Fernandez, Céline; Krogh, Morten LU ; Wårell, Kristofer LU ; Alm, Kersti LU ; Oredsson, Stina LU ; Persson, Lo LU ; James, Peter LU and Holm, Cecilia LU (2009) In Journal of Proteome Research 8. p.5008-5019
Abstract
Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization from lipid stores, is expressed in the liver and decreased hepatic insulin sensitivity has been reported in our HSL null mouse model. Here, an integrated approach, comprising transcriptomics and proteomics together with targeted metabolite analysis, was used to investigate the liver phenotype of HSL null mice. Oligonucleotide microarray analysis revealed altered expression of genes involved in lipid and polyamine metabolism in HSL null mice compared with wild-type mice and in genes controlling the immune system in mice on high-fat diet versus mice on normal diet. Two-dimensional gel electrophoresis followed by MS and/or MS/MS allowed identification of 52 and 22 unique... (More)
Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization from lipid stores, is expressed in the liver and decreased hepatic insulin sensitivity has been reported in our HSL null mouse model. Here, an integrated approach, comprising transcriptomics and proteomics together with targeted metabolite analysis, was used to investigate the liver phenotype of HSL null mice. Oligonucleotide microarray analysis revealed altered expression of genes involved in lipid and polyamine metabolism in HSL null mice compared with wild-type mice and in genes controlling the immune system in mice on high-fat diet versus mice on normal diet. Two-dimensional gel electrophoresis followed by MS and/or MS/MS allowed identification of 52 and 22 unique proteins differentially regulated according to the genotype and diet, respectively. Changes were observed mainly for proteins related to metabolism, including several proteins involved in polyamine metabolism or exhibiting methyl transferase activity. Despite the coordinated changes in mRNA and protein levels in polyamine pathways, no significant differences in levels of key polyamine metabolites were detected between the two genotypes. This study identifies a link between HSL and polyamine metabolism, which deserves further attention in view of the emerging data suggesting that disturbances in polyamine metabolism may affect insulin sensitivity. The present work also describes a limited correlation between mRNA, protein and metabolite levels, thus, underscoring the importance of integrated approaches. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteome Research
volume
8
pages
5008 - 5019
publisher
The American Chemical Society
external identifiers
  • wos:000276949600010
  • pmid:19785415
  • scopus:70449360655
ISSN
1535-3893
DOI
10.1021/pr9004037
language
English
LU publication?
yes
id
e672da25-2605-41a9-9077-976e74c8ebfb (old id 1500954)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19785415?dopt=Abstract
date added to LUP
2009-11-04 12:29:29
date last changed
2017-01-01 07:30:12
@article{e672da25-2605-41a9-9077-976e74c8ebfb,
  abstract     = {Hormone-sensitive lipase (HSL), a key enzyme in fatty acid mobilization from lipid stores, is expressed in the liver and decreased hepatic insulin sensitivity has been reported in our HSL null mouse model. Here, an integrated approach, comprising transcriptomics and proteomics together with targeted metabolite analysis, was used to investigate the liver phenotype of HSL null mice. Oligonucleotide microarray analysis revealed altered expression of genes involved in lipid and polyamine metabolism in HSL null mice compared with wild-type mice and in genes controlling the immune system in mice on high-fat diet versus mice on normal diet. Two-dimensional gel electrophoresis followed by MS and/or MS/MS allowed identification of 52 and 22 unique proteins differentially regulated according to the genotype and diet, respectively. Changes were observed mainly for proteins related to metabolism, including several proteins involved in polyamine metabolism or exhibiting methyl transferase activity. Despite the coordinated changes in mRNA and protein levels in polyamine pathways, no significant differences in levels of key polyamine metabolites were detected between the two genotypes. This study identifies a link between HSL and polyamine metabolism, which deserves further attention in view of the emerging data suggesting that disturbances in polyamine metabolism may affect insulin sensitivity. The present work also describes a limited correlation between mRNA, protein and metabolite levels, thus, underscoring the importance of integrated approaches.},
  author       = {Fernandez, Céline and Krogh, Morten and Wårell, Kristofer and Alm, Kersti and Oredsson, Stina and Persson, Lo and James, Peter and Holm, Cecilia},
  issn         = {1535-3893},
  language     = {eng},
  pages        = {5008--5019},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Omics Analyses Reveal a Potential Link between Hormone-Sensitive Lipase and Polyamine Metabolism.},
  url          = {http://dx.doi.org/10.1021/pr9004037},
  volume       = {8},
  year         = {2009},
}