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Two-dimensional gel electrophoresis of proteins and peptides in bovine milk

Lindmark Månsson, Helena LU ; Timgren, Anna LU ; Alden, G and Paulsson, Marie LU (2005) In International Dairy Journal 15(2). p.111-121
Abstract
Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first dimension, while 15% T homogeneous gels were used in the second dimension. 2-DE evaluation of samples with various somatic cell counts showed an increased number of peptides with increased cell count. 2-DE analysis of samples heated at 65 and 85 degreesC for 30 min showed a decrease in the number of proteins and peptides with increased... (More)
Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first dimension, while 15% T homogeneous gels were used in the second dimension. 2-DE evaluation of samples with various somatic cell counts showed an increased number of peptides with increased cell count. 2-DE analysis of samples heated at 65 and 85 degreesC for 30 min showed a decrease in the number of proteins and peptides with increased temperature. Colostrum exhibited a peptide pattern in which the intensity and number of spots decreased with time postpartum. These results show that 2-DE is a useful tool to detect variations of proteins and peptides in milk. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Peptides, Proteins, Somatic cell counts, Two-dimensional gel electrophoresis, Colostrum, Bovine milk, Heat treatment
in
International Dairy Journal
volume
15
issue
2
pages
111 - 121
publisher
Elsevier
external identifiers
  • wos:000226570900002
  • scopus:11844284815
ISSN
0958-6946
DOI
10.1016/j.idairyj.2004.06.010
language
English
LU publication?
yes
id
a1261cdd-a892-40a8-82db-bf4e719fee4a (old id 152079)
date added to LUP
2007-07-18 15:35:14
date last changed
2017-07-30 04:40:14
@article{a1261cdd-a892-40a8-82db-bf4e719fee4a,
  abstract     = {Proteins and peptides in bovine milk and colostrum were separated by two-dimensional gel electrophoresis (2-DE). The proteins were separated in the first dimension by isoelectric focusing in the pH ranges 4-7 and 6-11, and in the second dimension by sodium dodecyl sulphate polyacrylamide gel electrophoresis on 12.5% T homogeneous gels. The peptides were separated in the pH range 3-10 in the first dimension, while 15% T homogeneous gels were used in the second dimension. 2-DE evaluation of samples with various somatic cell counts showed an increased number of peptides with increased cell count. 2-DE analysis of samples heated at 65 and 85 degreesC for 30 min showed a decrease in the number of proteins and peptides with increased temperature. Colostrum exhibited a peptide pattern in which the intensity and number of spots decreased with time postpartum. These results show that 2-DE is a useful tool to detect variations of proteins and peptides in milk.},
  author       = {Lindmark Månsson, Helena and Timgren, Anna and Alden, G and Paulsson, Marie},
  issn         = {0958-6946},
  keyword      = {Peptides,Proteins,Somatic cell counts,Two-dimensional gel electrophoresis,Colostrum,Bovine milk,Heat treatment},
  language     = {eng},
  number       = {2},
  pages        = {111--121},
  publisher    = {Elsevier},
  series       = {International Dairy Journal},
  title        = {Two-dimensional gel electrophoresis of proteins and peptides in bovine milk},
  url          = {http://dx.doi.org/10.1016/j.idairyj.2004.06.010},
  volume       = {15},
  year         = {2005},
}