Glucose-stimulated insulin secretion correlates with beta-cell lipolysis.
(2006) In Nutrition Metabolism and Cardiovascular Diseases 16. p.11-16- Abstract
- Background and aims: Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cell and in mouse islets. Methods and results: INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM, or 16.7 mM... (More)
- Background and aims: Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cell and in mouse islets. Methods and results: INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM, or 16.7 mM glucose for 1 h. Medium samples were collected and analyzed for insulin and glycerol. Triglycerides were measured in both INS-1 cells and islets. There was a dose-dependent glucose-stimulated lipolysis in INS-1 cells, which strongly correlated with insulin secretion (r = 0.85, P < 0.0001). The same phenomenon was observed in mouse islets (r = 0.9, P = 0.013). Low levels of triglycerides, which were observed in INS-1 cells pre-cultured at 3.3 mM glucose, were associated with reduced GSIS. Conclusions: This study suggests that lipids obtained from lipolysis of intracellular triglycerides are involved in GSIS. (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/154729
- author
- Sörhede Winzell, Maria LU ; Ström, Kristoffer LU ; Holm, Cecilia LU and Ahrén, Bo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- insulin secretion, glycerol, islets, INS-1 cells, triglycerides
- in
- Nutrition Metabolism and Cardiovascular Diseases
- volume
- 16
- pages
- 11 - 16
- publisher
- Elsevier
- external identifiers
-
- wos:000236769800004
- scopus:33645458999
- pmid:16530123
- ISSN
- 1590-3729
- DOI
- 10.1016/j.numecd.2005.11.006
- language
- English
- LU publication?
- yes
- id
- 94fbf24d-cf18-4e9e-b2ca-fb6c359d9714 (old id 154729)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16530123&dopt=Abstract
- date added to LUP
- 2016-04-01 12:15:38
- date last changed
- 2024-01-08 13:57:43
@article{94fbf24d-cf18-4e9e-b2ca-fb6c359d9714, abstract = {{Background and aims: Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cell and in mouse islets. Methods and results: INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM, or 16.7 mM glucose for 1 h. Medium samples were collected and analyzed for insulin and glycerol. Triglycerides were measured in both INS-1 cells and islets. There was a dose-dependent glucose-stimulated lipolysis in INS-1 cells, which strongly correlated with insulin secretion (r = 0.85, P < 0.0001). The same phenomenon was observed in mouse islets (r = 0.9, P = 0.013). Low levels of triglycerides, which were observed in INS-1 cells pre-cultured at 3.3 mM glucose, were associated with reduced GSIS. Conclusions: This study suggests that lipids obtained from lipolysis of intracellular triglycerides are involved in GSIS. (c) 2005 Elsevier B.V. All rights reserved.}}, author = {{Sörhede Winzell, Maria and Ström, Kristoffer and Holm, Cecilia and Ahrén, Bo}}, issn = {{1590-3729}}, keywords = {{insulin secretion; glycerol; islets; INS-1 cells; triglycerides}}, language = {{eng}}, pages = {{11--16}}, publisher = {{Elsevier}}, series = {{Nutrition Metabolism and Cardiovascular Diseases}}, title = {{Glucose-stimulated insulin secretion correlates with beta-cell lipolysis.}}, url = {{http://dx.doi.org/10.1016/j.numecd.2005.11.006}}, doi = {{10.1016/j.numecd.2005.11.006}}, volume = {{16}}, year = {{2006}}, }