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Glucose-stimulated insulin secretion correlates with beta-cell lipolysis.

Sörhede Winzell, Maria LU ; Ström, Kristoffer LU ; Holm, Cecilia LU and Ahrén, Bo LU (2006) In Nutrition Metabolism and Cardiovascular Diseases 16. p.11-16
Abstract
Background and aims: Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cell and in mouse islets. Methods and results: INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM, or 16.7 mM... (More)
Background and aims: Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cell and in mouse islets. Methods and results: INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM, or 16.7 mM glucose for 1 h. Medium samples were collected and analyzed for insulin and glycerol. Triglycerides were measured in both INS-1 cells and islets. There was a dose-dependent glucose-stimulated lipolysis in INS-1 cells, which strongly correlated with insulin secretion (r = 0.85, P < 0.0001). The same phenomenon was observed in mouse islets (r = 0.9, P = 0.013). Low levels of triglycerides, which were observed in INS-1 cells pre-cultured at 3.3 mM glucose, were associated with reduced GSIS. Conclusions: This study suggests that lipids obtained from lipolysis of intracellular triglycerides are involved in GSIS. (c) 2005 Elsevier B.V. All rights reserved. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
insulin secretion, glycerol, islets, INS-1 cells, triglycerides
in
Nutrition Metabolism and Cardiovascular Diseases
volume
16
pages
11 - 16
publisher
Elsevier
external identifiers
  • wos:000236769800004
  • scopus:33645458999
  • pmid:16530123
ISSN
1590-3729
DOI
10.1016/j.numecd.2005.11.006
language
English
LU publication?
yes
id
94fbf24d-cf18-4e9e-b2ca-fb6c359d9714 (old id 154729)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16530123&dopt=Abstract
date added to LUP
2016-04-01 12:15:38
date last changed
2021-08-04 05:20:42
@article{94fbf24d-cf18-4e9e-b2ca-fb6c359d9714,
  abstract     = {Background and aims: Lipids are needed for optimal glucose-stimulated insulin secretion (GSIS), and long-chain acyl-CoA (LC-CoA) has been suggested as one candidate molecule active as a lipidic coupling factor. LC-CoAs may be available to the beta-cell via uptake of circulating free fatty acids or from hydrolysis of intracellularly stored triglycerides. Inhibition of lipolysis in rat islets using a non-specific lipase inhibitor (orlistat) resulted in blunted GSIS. The aim of this study was to investigate the relationship between GSIS and lipolysis in clonal beta-cell and in mouse islets. Methods and results: INS-1 cells, cultured overnight at 3.3 mM or 11.1 mM glucose, or freshly isolated islets were incubated with 3.3 mM, or 16.7 mM glucose for 1 h. Medium samples were collected and analyzed for insulin and glycerol. Triglycerides were measured in both INS-1 cells and islets. There was a dose-dependent glucose-stimulated lipolysis in INS-1 cells, which strongly correlated with insulin secretion (r = 0.85, P &lt; 0.0001). The same phenomenon was observed in mouse islets (r = 0.9, P = 0.013). Low levels of triglycerides, which were observed in INS-1 cells pre-cultured at 3.3 mM glucose, were associated with reduced GSIS. Conclusions: This study suggests that lipids obtained from lipolysis of intracellular triglycerides are involved in GSIS. (c) 2005 Elsevier B.V. All rights reserved.},
  author       = {Sörhede Winzell, Maria and Ström, Kristoffer and Holm, Cecilia and Ahrén, Bo},
  issn         = {1590-3729},
  language     = {eng},
  pages        = {11--16},
  publisher    = {Elsevier},
  series       = {Nutrition Metabolism and Cardiovascular Diseases},
  title        = {Glucose-stimulated insulin secretion correlates with beta-cell lipolysis.},
  url          = {http://dx.doi.org/10.1016/j.numecd.2005.11.006},
  doi          = {10.1016/j.numecd.2005.11.006},
  volume       = {16},
  year         = {2006},
}