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Bile duct obstruction is associated with early postoperative upregulation of liver uncoupling protein-2 and reduced circulating glucose concentration in the rat

Enochsson, Lars; Isaksson, Bengt; Strommer, Lovisa; Erlanson-Albertsson, Charlotte LU and Permert, Johan (2010) In Nutrition 26(4). p.405-410
Abstract
Objective: To evaluate whether upregulation of liver and muscle uncoupling protein 2 (UCP-2) is an acute phenomenon in obstructive jaundice and associated with secondary metabolic effects. Methods: Male Sprague-Dawley rats were divided into four groups bile duct ligated (BDL) and sham-operated pair-fed (PF), ad libitum fed (AL), and controls BDL. PF. and AL rats were further divided into subgroups according to the interval postoperatively when they were reanesthetized and sampled for tissue and blood 2, 4, and 8 d, respectively Bilirubin, liver enzymes. glucose, free fatty acids, and insulin in blood plasma were analyzed Liver and muscle tissue were sampled for UCP-2 and adenosine triphosphate analysis. Results: The BDL rats showed an... (More)
Objective: To evaluate whether upregulation of liver and muscle uncoupling protein 2 (UCP-2) is an acute phenomenon in obstructive jaundice and associated with secondary metabolic effects. Methods: Male Sprague-Dawley rats were divided into four groups bile duct ligated (BDL) and sham-operated pair-fed (PF), ad libitum fed (AL), and controls BDL. PF. and AL rats were further divided into subgroups according to the interval postoperatively when they were reanesthetized and sampled for tissue and blood 2, 4, and 8 d, respectively Bilirubin, liver enzymes. glucose, free fatty acids, and insulin in blood plasma were analyzed Liver and muscle tissue were sampled for UCP-2 and adenosine triphosphate analysis. Results: The BDL rats showed an increase of the liver UCP-2 expression compared with PF and AL rats (P < 0 05) 4 d postoperatively. Liver adenosine triphosphate in BDL rats showed a decrease compared with sham-operated controls at all intervals < 0 05) Plasma glucose concentration in BDL rats was decreased compared with the other groups. Free fatty acids showed an initial increase 2 d postoperatively compared with sham-operated controls and PF and AL rats (P < 0 05) at the corresponding time point Conclusion: Obstructive jaundice is associated with an early upregulation of liver UCP-2. reduced liver adenosine triphosphate content, and decreased plasma glucose concentration, supporting the hypothesis that obstructive Jaundice results in impaired energy homeostasis in the liver. which might cause decreased glucose output and hypoglycemia as a consequence (C) 2010 Elsevier Inc. All flats reserved (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein, Uncoupling, Obstructive jaundice, Bile duct ligation, Mitochondria
in
Nutrition
volume
26
issue
4
pages
405 - 410
publisher
Elsevier
external identifiers
  • wos:000277704200009
  • scopus:77349087797
ISSN
1873-1244
DOI
10.1016/j.nut.2009.05.005
language
English
LU publication?
yes
id
e9cdd55e-7431-48f8-b9a9-01a3bb6f0978 (old id 1617311)
date added to LUP
2010-06-22 11:52:34
date last changed
2018-05-29 10:07:09
@article{e9cdd55e-7431-48f8-b9a9-01a3bb6f0978,
  abstract     = {Objective: To evaluate whether upregulation of liver and muscle uncoupling protein 2 (UCP-2) is an acute phenomenon in obstructive jaundice and associated with secondary metabolic effects. Methods: Male Sprague-Dawley rats were divided into four groups bile duct ligated (BDL) and sham-operated pair-fed (PF), ad libitum fed (AL), and controls BDL. PF. and AL rats were further divided into subgroups according to the interval postoperatively when they were reanesthetized and sampled for tissue and blood 2, 4, and 8 d, respectively Bilirubin, liver enzymes. glucose, free fatty acids, and insulin in blood plasma were analyzed Liver and muscle tissue were sampled for UCP-2 and adenosine triphosphate analysis. Results: The BDL rats showed an increase of the liver UCP-2 expression compared with PF and AL rats (P &lt; 0 05) 4 d postoperatively. Liver adenosine triphosphate in BDL rats showed a decrease compared with sham-operated controls at all intervals &lt; 0 05) Plasma glucose concentration in BDL rats was decreased compared with the other groups. Free fatty acids showed an initial increase 2 d postoperatively compared with sham-operated controls and PF and AL rats (P &lt; 0 05) at the corresponding time point Conclusion: Obstructive jaundice is associated with an early upregulation of liver UCP-2. reduced liver adenosine triphosphate content, and decreased plasma glucose concentration, supporting the hypothesis that obstructive Jaundice results in impaired energy homeostasis in the liver. which might cause decreased glucose output and hypoglycemia as a consequence (C) 2010 Elsevier Inc. All flats reserved},
  author       = {Enochsson, Lars and Isaksson, Bengt and Strommer, Lovisa and Erlanson-Albertsson, Charlotte and Permert, Johan},
  issn         = {1873-1244},
  keyword      = {protein,Uncoupling,Obstructive jaundice,Bile duct ligation,Mitochondria},
  language     = {eng},
  number       = {4},
  pages        = {405--410},
  publisher    = {Elsevier},
  series       = {Nutrition},
  title        = {Bile duct obstruction is associated with early postoperative upregulation of liver uncoupling protein-2 and reduced circulating glucose concentration in the rat},
  url          = {http://dx.doi.org/10.1016/j.nut.2009.05.005},
  volume       = {26},
  year         = {2010},
}