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Ghrelin affects gastrectomy-induced decrease in UCP1 and beta(3)-AR mRNA expression in mice.

Lindqvist, Andreas LU ; de la Cour, Charlotta LU ; Håkanson, Rolf LU and Erlanson-Albertsson, Charlotte LU (2007) In Regulatory Peptides 142(1-2). p.24-28
Abstract
this study we investigated the effects of gastrectomy (Gx) and of the gastric hormone, ghrelin, on the expression of proteins in brown adipose tissue (BAT) that are thought to be involved in thermogenesis. Heat production in BAT is known to depend upon activation and increased expression of β3-adrenergic receptors (β3-AR) and the consequent up-regulation of uncoupling protein 1 (UCP1). Mice were subjected to Gx or sham operation. One week later they started to receive daily subcutaneous injections of either saline or ghrelin (12 nmol) for two or eight weeks. Neither Gx nor ghrelin affected daily food intake. Gx did not lower body weight gain (except during the first post-operative week) but Gx mice responded to eight weeks of ghrelin... (More)
this study we investigated the effects of gastrectomy (Gx) and of the gastric hormone, ghrelin, on the expression of proteins in brown adipose tissue (BAT) that are thought to be involved in thermogenesis. Heat production in BAT is known to depend upon activation and increased expression of β3-adrenergic receptors (β3-AR) and the consequent up-regulation of uncoupling protein 1 (UCP1). Mice were subjected to Gx or sham operation. One week later they started to receive daily subcutaneous injections of either saline or ghrelin (12 nmol) for two or eight weeks. Neither Gx nor ghrelin affected daily food intake. Gx did not lower body weight gain (except during the first post-operative week) but Gx mice responded to eight weeks of ghrelin treatment with a greater body weight increase (37%, p < 0.05) than saline-injected Gx mice; sham-operated mice did not respond to ghrelin. Gx resulted in a greatly reduced expression of both UCP1 and β3-AR mRNA in BAT (50% reduction or more, p < 0.01) compared to sham-operated mice. Eight weeks of ghrelin treatment raised the UCP1 as well as the β3-AR mRNA expression in the Gx mice, whereas two weeks of ghrelin treatment decreased UCP1 and β3-AR mRNA expression compared to Gx mice receiving saline. In fact, mRNA expression in Gx mice after treatment with ghrelin for eight weeks was similar to that in saline-treated sham-operated mice. Ghrelin did not affect UCP1 and β3-AR mRNA in sham-operated mice neither two nor eight weeks after the operation. The results suggest 1) that signals from the stomach stimulate BAT UCP1 (and possibly thermogenesis) and 2) that ghrelin may contribute to the control of UCP1 expression. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
β3-adrenergic receptor, Ghrelin, UCP1, Thermogenesis
in
Regulatory Peptides
volume
142
issue
1-2
pages
24 - 28
publisher
Elsevier
external identifiers
  • wos:000247365000004
  • scopus:34248575910
ISSN
1873-1686
DOI
10.1016/j.regpep.2007.01.007
language
English
LU publication?
yes
id
1e811f8d-3c8d-4c08-9018-5354a66cb4c6 (old id 166953)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17328973&dopt=Abstract
date added to LUP
2007-07-17 08:56:43
date last changed
2017-07-30 03:32:37
@article{1e811f8d-3c8d-4c08-9018-5354a66cb4c6,
  abstract     = {this study we investigated the effects of gastrectomy (Gx) and of the gastric hormone, ghrelin, on the expression of proteins in brown adipose tissue (BAT) that are thought to be involved in thermogenesis. Heat production in BAT is known to depend upon activation and increased expression of β3-adrenergic receptors (β3-AR) and the consequent up-regulation of uncoupling protein 1 (UCP1). Mice were subjected to Gx or sham operation. One week later they started to receive daily subcutaneous injections of either saline or ghrelin (12 nmol) for two or eight weeks. Neither Gx nor ghrelin affected daily food intake. Gx did not lower body weight gain (except during the first post-operative week) but Gx mice responded to eight weeks of ghrelin treatment with a greater body weight increase (37%, p &lt; 0.05) than saline-injected Gx mice; sham-operated mice did not respond to ghrelin. Gx resulted in a greatly reduced expression of both UCP1 and β3-AR mRNA in BAT (50% reduction or more, p &lt; 0.01) compared to sham-operated mice. Eight weeks of ghrelin treatment raised the UCP1 as well as the β3-AR mRNA expression in the Gx mice, whereas two weeks of ghrelin treatment decreased UCP1 and β3-AR mRNA expression compared to Gx mice receiving saline. In fact, mRNA expression in Gx mice after treatment with ghrelin for eight weeks was similar to that in saline-treated sham-operated mice. Ghrelin did not affect UCP1 and β3-AR mRNA in sham-operated mice neither two nor eight weeks after the operation. The results suggest 1) that signals from the stomach stimulate BAT UCP1 (and possibly thermogenesis) and 2) that ghrelin may contribute to the control of UCP1 expression.},
  author       = {Lindqvist, Andreas and de la Cour, Charlotta and Håkanson, Rolf and Erlanson-Albertsson, Charlotte},
  issn         = {1873-1686},
  keyword      = {β3-adrenergic receptor,Ghrelin,UCP1,Thermogenesis},
  language     = {eng},
  number       = {1-2},
  pages        = {24--28},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Ghrelin affects gastrectomy-induced decrease in UCP1 and beta(3)-AR mRNA expression in mice.},
  url          = {http://dx.doi.org/10.1016/j.regpep.2007.01.007},
  volume       = {142},
  year         = {2007},
}