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Separation of Selenium, Zinc, and Copper Compounds in Bovine Whey Using Size Exclusion Chromatography Linked to Inductively Coupled Plasma Mass Spectrometry.

Hoac, Tien LU ; Lundh, Thomas LU ; Purup, Stig; Önning, Gunilla LU ; Sejrsen, Kristen and Åkesson, Björn LU (2007) In Journal of Agricultural and Food Chemistry 55(10). p.4237-4243
Abstract
To study the role of trace elements for the quality and nutritional value of bovine milk, the distribution of selenium, zinc, and copper in whey was investigated using a method linking size exclusion chromatography to inductively coupled plasma mass spectrometry (SEC-ICP-MS). Three major peaks were detected for selenium, two peaks for zinc, and five peaks for copper. More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. All zinc was associated with low molecular weight compounds (< 5 kDa) and one of these compounds was probably citrate. More than 60% of the copper eluted in protein fractions and two of the five major peaks... (More)
To study the role of trace elements for the quality and nutritional value of bovine milk, the distribution of selenium, zinc, and copper in whey was investigated using a method linking size exclusion chromatography to inductively coupled plasma mass spectrometry (SEC-ICP-MS). Three major peaks were detected for selenium, two peaks for zinc, and five peaks for copper. More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. All zinc was associated with low molecular weight compounds (< 5 kDa) and one of these compounds was probably citrate. More than 60% of the copper eluted in protein fractions and two of the five major peaks probably contained metallothionein and citrate. This method was used to compare milk and whey produced by organic and conventional feeding procedures. The selenium content in whey and desalted milk produced using organic regimens was significantly lower than that in conventional samples. Moreover, the proportion of selenium in protein fractions of organic whey was significantly smaller than that in conventional whey, but the distributions of zinc and copper did not differ. This study showed that with the SEC-ICP-MS technique the distribution profiles of several trace elements in whey could be studied in the same run and that the selenium profile differed in whey produced by organic and conventional procedures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
trace element distribution, milk, whey, ICP-MS, selenium, copper, zinc
in
Journal of Agricultural and Food Chemistry
volume
55
issue
10
pages
4237 - 4243
publisher
The American Chemical Society
external identifiers
  • wos:000246313200070
  • scopus:34249914819
ISSN
0021-8561
DOI
10.1021/jf070169x
language
English
LU publication?
yes
id
c03222b9-ef1f-48c0-b7ba-61846bbb56a7 (old id 167492)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17447791&dopt=Abstract
date added to LUP
2007-07-12 08:42:12
date last changed
2017-06-18 03:45:22
@article{c03222b9-ef1f-48c0-b7ba-61846bbb56a7,
  abstract     = {To study the role of trace elements for the quality and nutritional value of bovine milk, the distribution of selenium, zinc, and copper in whey was investigated using a method linking size exclusion chromatography to inductively coupled plasma mass spectrometry (SEC-ICP-MS). Three major peaks were detected for selenium, two peaks for zinc, and five peaks for copper. More than 65% of the selenium was found in protein fractions, mainly in fractions coinciding with the major whey proteins beta-lactoglobulin and alpha-lactalbumin. All zinc was associated with low molecular weight compounds (&lt; 5 kDa) and one of these compounds was probably citrate. More than 60% of the copper eluted in protein fractions and two of the five major peaks probably contained metallothionein and citrate. This method was used to compare milk and whey produced by organic and conventional feeding procedures. The selenium content in whey and desalted milk produced using organic regimens was significantly lower than that in conventional samples. Moreover, the proportion of selenium in protein fractions of organic whey was significantly smaller than that in conventional whey, but the distributions of zinc and copper did not differ. This study showed that with the SEC-ICP-MS technique the distribution profiles of several trace elements in whey could be studied in the same run and that the selenium profile differed in whey produced by organic and conventional procedures.},
  author       = {Hoac, Tien and Lundh, Thomas and Purup, Stig and Önning, Gunilla and Sejrsen, Kristen and Åkesson, Björn},
  issn         = {0021-8561},
  keyword      = {trace element distribution,milk,whey,ICP-MS,selenium,copper,zinc},
  language     = {eng},
  number       = {10},
  pages        = {4237--4243},
  publisher    = {The American Chemical Society},
  series       = {Journal of Agricultural and Food Chemistry},
  title        = {Separation of Selenium, Zinc, and Copper Compounds in Bovine Whey Using Size Exclusion Chromatography Linked to Inductively Coupled Plasma Mass Spectrometry.},
  url          = {http://dx.doi.org/10.1021/jf070169x},
  volume       = {55},
  year         = {2007},
}