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Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate.

Kåredal, Monica LU ; Mörtstedt, Harriet LU ; Jeppsson, Marina LU ; Diab, Kerstin LU ; Nielsen, Jörn LU ; Jönsson, Bo A LU and Lindh, Christian LU (2010) In Journal of Proteome Research okt. p.5620-5628
Abstract
Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples... (More)
Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteome Research
volume
okt
pages
5620 - 5628
publisher
The American Chemical Society
external identifiers
  • wos:000283810500011
  • pmid:20815409
  • scopus:78149393619
ISSN
1535-3893
DOI
10.1021/pr100436a
language
English
LU publication?
yes
id
7e77ce40-173d-4a02-9701-b9069e151fda (old id 1688570)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/20815409?dopt=Abstract
date added to LUP
2010-10-05 08:42:35
date last changed
2018-05-29 09:55:49
@article{7e77ce40-173d-4a02-9701-b9069e151fda,
  abstract     = {Hairdressers are frequently exposed to bleaching powder containing persulfates, a group of compounds that may induce hypersensitivity in the airways. The mechanism causing this reaction is not clear. The aim of this study was to identify changes in the nasal lavage fluid proteome after challenge with potassium persulfate in hairdressers with bleaching powder-associated rhinitis. Furthermore, we aimed to compare their response to that of hairdressers without nasal symptoms, and atopic subjects with pollen-associated nasal symptoms. To study the pathogenesis of persulfate-associated rhinitis, the response in protein expression from the upper airway was assessed by time-dependent proteomic expression analysis of nasal lavage fluids. Samples were prepared by pooling nasal lavage fluids from the groups at different time points after challenge. Samples were depleted of high-abundant proteins, labeled with iTRAQ and analyzed by online 2D-nanoLC-MS/MS. Differences in the protein pattern between the three groups were observed. Most proteins with differentially expressed levels were involved in pathways of lipid transportation and antimicrobial activities. The major finding was increased abundance of apolipoprotein A-1, 20 min postchallenge, detected solely in the group of symptomatic hairdressers. Our results suggest there may be differences between the mechanisms responsible for the rhinitis in the symptomatic and atopic group.},
  author       = {Kåredal, Monica and Mörtstedt, Harriet and Jeppsson, Marina and Diab, Kerstin and Nielsen, Jörn and Jönsson, Bo A and Lindh, Christian},
  issn         = {1535-3893},
  language     = {eng},
  pages        = {5620--5628},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Time-Dependent Proteomic iTRAQ Analysis of Nasal Lavage of Hairdressers Challenged by Persulfate.},
  url          = {http://dx.doi.org/10.1021/pr100436a},
  volume       = {okt},
  year         = {2010},
}