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Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.

Bolmeson, Caroline LU ; Esguerra, Jonathan LU ; Salehi, S Albert LU ; Speidel, Dina LU ; Eliasson, Lena LU and Cilio, Corrado LU (2011) In Biochemical and Biophysical Research Communications Dec. p.16-22
Abstract
Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors... (More)
Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
Dec
pages
16 - 22
publisher
Elsevier
external identifiers
  • wos:000286487700004
  • pmid:21094635
  • scopus:78650902669
ISSN
1090-2104
DOI
10.1016/j.bbrc.2010.11.024
language
English
LU publication?
yes
id
9ffc6bca-c591-4b85-a04b-a94f6b0eb346 (old id 1731717)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21094635?dopt=Abstract
date added to LUP
2010-12-01 13:27:15
date last changed
2017-07-09 04:35:54
@article{9ffc6bca-c591-4b85-a04b-a94f6b0eb346,
  abstract     = {Many microRNAs (miRNAs) are known to be cell-type specific and are implicated in development of diseases. We investigated the global expression pattern of miRNAs in human pancreatic islets compared to liver and skeletal muscle, using bead-based technology and quantitative RT-PCR. In addition to the known islet-specific miR-375, we also found enrichment of miR-127-3p, miR-184, miR-195 and miR-493∗ in the pancreatic islets. The expression of miR-375, miR-127-3p, miR-184 and the liver-enriched miR-122 were positively correlated to insulin biosynthesis, while the expression of miR-127-3p and miR-184 were negatively correlated to glucose-stimulated insulin secretion (GSIS). These correlations were absent in islets of glucose intolerant donors (HbA1c⩾6.1). We suggest the presence of an islet-specific miRNA network, which consists of at least miR-375, miR-127-3p and miR-184, potentially involved in insulin secretion. Our results provide new insight into miRNA-mediated regulation of insulin secretion in healthy and glucose intolerant subjects.},
  author       = {Bolmeson, Caroline and Esguerra, Jonathan and Salehi, S Albert and Speidel, Dina and Eliasson, Lena and Cilio, Corrado},
  issn         = {1090-2104},
  language     = {eng},
  pages        = {16--22},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Differences in islet-enriched miRNAs in healthy and glucose intolerant human subjects.},
  url          = {http://dx.doi.org/10.1016/j.bbrc.2010.11.024},
  volume       = {Dec},
  year         = {2011},
}