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Effects on weight gain and gut microbiota in rats given bacterial supplements and a high-energy-dense diet from fetal life through to 6 months of age

Linninge, Caroline LU ; Molin, Göran LU ; Fåk, Frida LU ; Johansson Hagslätt, Marie-Louise; Jakesevic, Maja LU ; Håkansson, Åsa LU ; Jeppsson, Bengt LU ; Weström, Björn LU and Ahrné, Siv LU (2011) In British Journal of Nutrition 106(6). p.887-895
Abstract
The aim of this study was to assess the long-term effects of a high-energy dense diet, supplemented with Lactobacillus plantarum (Lp) or Escherichia coli (Ec) on weight gain, fattening and the gut microbiota in rats. Since the mother’s dietary habits can influence offspring physiology, the dietary regimes started with the dams at pregnancy and through lactation, and continued with the offspring for six months. The weight gain of group Lp was lower than for groups C (control) and Ec (P=0•086). More retroperitoneal adipose tissue (P=0•030) and higher plasma leptin (P=0•035) were seen in group Ec compared to group Lp. The viable count of Enterobacteriaceae was higher in group Ec than in group Lp (P=0•019) and when all animals were compared,... (More)
The aim of this study was to assess the long-term effects of a high-energy dense diet, supplemented with Lactobacillus plantarum (Lp) or Escherichia coli (Ec) on weight gain, fattening and the gut microbiota in rats. Since the mother’s dietary habits can influence offspring physiology, the dietary regimes started with the dams at pregnancy and through lactation, and continued with the offspring for six months. The weight gain of group Lp was lower than for groups C (control) and Ec (P=0•086). More retroperitoneal adipose tissue (P=0•030) and higher plasma leptin (P=0•035) were seen in group Ec compared to group Lp. The viable count of Enterobacteriaceae was higher in group Ec than in group Lp (P=0•019) and when all animals were compared, Enterobacteriaceae correlated positively with body weight (r=0•428, P=0•029). Bacterial diversity was lower in group Ec than in groups C (P=<0•05) and Lp (P=<0•05). Firmicutes, Bacteroidetes and Verrucomicrobia dominated in all groups, but Bacteroidetes were more prevalent in group C than in groups Lp (P=0•036) and Ec (P=0•056). The same five bacterial families dominated the microbiota of groups Ec and C, and four of these were also present in group Lp. The other five families dominating in group Lp were not found in any of the other groups. Multivariate data analysis pointed in the same directions as the univariate statistics. Our results suggest that supplementation of L. plantarum or E. coli can have long-term effects on the composition of the intestinal microbiota, as well as on weight gain and fattening. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
body weight, probiotics, biochemical markers, gut microbiota, high-energy dense diet
in
British Journal of Nutrition
volume
106
issue
6
pages
887 - 895
publisher
Cambridge University Press
external identifiers
  • wos:000295403000013
  • scopus:80555156674
ISSN
1475-2662
DOI
10.1017/S0007114511001036
language
English
LU publication?
yes
id
7fcd9695-dc00-46ec-a747-622e39c781c8 (old id 1890839)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21450114?dopt=Abstract
date added to LUP
2011-04-20 09:32:19
date last changed
2017-11-19 03:00:45
@article{7fcd9695-dc00-46ec-a747-622e39c781c8,
  abstract     = {The aim of this study was to assess the long-term effects of a high-energy dense diet, supplemented with Lactobacillus plantarum (Lp) or Escherichia coli (Ec) on weight gain, fattening and the gut microbiota in rats. Since the mother’s dietary habits can influence offspring physiology, the dietary regimes started with the dams at pregnancy and through lactation, and continued with the offspring for six months. The weight gain of group Lp was lower than for groups C (control) and Ec (P=0•086). More retroperitoneal adipose tissue (P=0•030) and higher plasma leptin (P=0•035) were seen in group Ec compared to group Lp. The viable count of Enterobacteriaceae was higher in group Ec than in group Lp (P=0•019) and when all animals were compared, Enterobacteriaceae correlated positively with body weight (r=0•428, P=0•029). Bacterial diversity was lower in group Ec than in groups C (P=&lt;0•05) and Lp (P=&lt;0•05). Firmicutes, Bacteroidetes and Verrucomicrobia dominated in all groups, but Bacteroidetes were more prevalent in group C than in groups Lp (P=0•036) and Ec (P=0•056). The same five bacterial families dominated the microbiota of groups Ec and C, and four of these were also present in group Lp. The other five families dominating in group Lp were not found in any of the other groups. Multivariate data analysis pointed in the same directions as the univariate statistics. Our results suggest that supplementation of L. plantarum or E. coli can have long-term effects on the composition of the intestinal microbiota, as well as on weight gain and fattening.},
  author       = {Linninge, Caroline and Molin, Göran and Fåk, Frida and Johansson Hagslätt, Marie-Louise and Jakesevic, Maja and Håkansson, Åsa and Jeppsson, Bengt and Weström, Björn and Ahrné, Siv},
  issn         = {1475-2662},
  keyword      = {body weight,probiotics,biochemical markers,gut microbiota,high-energy dense diet},
  language     = {eng},
  number       = {6},
  pages        = {887--895},
  publisher    = {Cambridge University Press},
  series       = {British Journal of Nutrition},
  title        = {Effects on weight gain and gut microbiota in rats given bacterial supplements and a high-energy-dense diet from fetal life through to 6 months of age},
  url          = {http://dx.doi.org/10.1017/S0007114511001036},
  volume       = {106},
  year         = {2011},
}