Early changes in gene expression in the dorsal root ganglia after transection of the sciatic nerve; effects of amphiregulin and PAI-1 on regeneration.
Nilsson, Anna LU ; Moller, Kristian LU ; Dahlin, Lars LU
; Lundborg, Göran
LU
and Kanje, Martin
LU
(2005)
In Brain Research. Molecular Brain Research
136(1-2).
p.65-74
- Abstract
- To characterize the gene activity that may be required for neuronal survival and regeneration, we used the Affymetrix GeneChip Mu74A to screen 12000 genes and expressed sequence tag (EST) mRNA from L4 and L5 mouse dorsal root ganglia (DRG) 12 h and 24 h after sciatic nerve transection. At 12 h, we found 17 upregulated transcripts, and at 24 h, 49 that met our criteria of a significant 2-fold increase in expression. The alterations included a total of eight transcription factors and several genes associated with TGF-beta- and IL-6-mediated signaling. Two of the changes, amphiregulin and plasminogen activator inhibitor-1 (PAI-1), were confirmed by real-time quantitative PCR (QPCR). Addition of amphiregulin (20 ng/ml) to organ-cultured DRG... (More)
- To characterize the gene activity that may be required for neuronal survival and regeneration, we used the Affymetrix GeneChip Mu74A to screen 12000 genes and expressed sequence tag (EST) mRNA from L4 and L5 mouse dorsal root ganglia (DRG) 12 h and 24 h after sciatic nerve transection. At 12 h, we found 17 upregulated transcripts, and at 24 h, 49 that met our criteria of a significant 2-fold increase in expression. The alterations included a total of eight transcription factors and several genes associated with TGF-beta- and IL-6-mediated signaling. Two of the changes, amphiregulin and plasminogen activator inhibitor-1 (PAI-1), were confirmed by real-time quantitative PCR (QPCR). Addition of amphiregulin (20 ng/ml) to organ-cultured DRG stimulated axonal outgrowth while PAI-1 (20 nM) inhibited migration of Schwann cells from the ganglia. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138014
- author
- Nilsson, Anna
LU
; Moller, Kristian
LU
; Dahlin, Lars
LU
; Lundborg, Göran
LU
and Kanje, Martin
LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Dorsal root ganglia, Gene, Micro-array, Mouse, Nerve regeneration
- in
- Brain Research. Molecular Brain Research
- volume
- 136
- issue
- 1-2
- pages
- 65 - 74
- publisher
- Elsevier
- external identifiers
-
- pmid:15893588
- wos:000229497400008
- scopus:19044374577
- pmid:15893588
- ISSN
- 0169-328X
- DOI
- 10.1016/j.molbrainres.2005.01.008
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hand Surgery Research Group (013241910), Functional Zoology (432112239), Medical Neurochemistry (ceased) (LUR000012), Department of Biology (000016100), Division of Clinical Chemistry and Pharmacology (013250300)
- id
- 1c10c016-1bd5-426d-9978-ffe8ec9fd84b (old id 138014)
- date added to LUP
- 2016-04-01 15:20:12
- date last changed
- 2022-01-28 04:48:47
@article{1c10c016-1bd5-426d-9978-ffe8ec9fd84b,
abstract = {{To characterize the gene activity that may be required for neuronal survival and regeneration, we used the Affymetrix GeneChip Mu74A to screen 12000 genes and expressed sequence tag (EST) mRNA from L4 and L5 mouse dorsal root ganglia (DRG) 12 h and 24 h after sciatic nerve transection. At 12 h, we found 17 upregulated transcripts, and at 24 h, 49 that met our criteria of a significant 2-fold increase in expression. The alterations included a total of eight transcription factors and several genes associated with TGF-beta- and IL-6-mediated signaling. Two of the changes, amphiregulin and plasminogen activator inhibitor-1 (PAI-1), were confirmed by real-time quantitative PCR (QPCR). Addition of amphiregulin (20 ng/ml) to organ-cultured DRG stimulated axonal outgrowth while PAI-1 (20 nM) inhibited migration of Schwann cells from the ganglia.}},
author = {{Nilsson, Anna and Moller, Kristian and Dahlin, Lars and Lundborg, Göran and Kanje, Martin}},
issn = {{0169-328X}},
keywords = {{Dorsal root ganglia; Gene; Micro-array; Mouse; Nerve regeneration}},
language = {{eng}},
number = {{1-2}},
pages = {{65--74}},
publisher = {{Elsevier}},
series = {{Brain Research. Molecular Brain Research}},
title = {{Early changes in gene expression in the dorsal root ganglia after transection of the sciatic nerve; effects of amphiregulin and PAI-1 on regeneration.}},
url = {{http://dx.doi.org/10.1016/j.molbrainres.2005.01.008}},
doi = {{10.1016/j.molbrainres.2005.01.008}},
volume = {{136}},
year = {{2005}},
}