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UROSCAN and UROSCANSEQ : a large-scale multicenter effort towards translation of molecular bladder cancer subtypes into clinical practice–from biobank to RNA-sequencing in real time

Liedberg, Fredrik LU ; Abrahamsson, Johan LU ; Bernardo, Carina LU orcid ; Bläckberg, Mats LU ; Edsjö, Anders LU ; Heidenblad, Markus LU ; Larsson, Christer LU ; Sjödahl, Gottfrid LU and Eriksson, Pontus LU (2023) In Scandinavian Journal of Urology 57(1-6). p.2-9
Abstract

Background: Bladder cancer is molecularly one of the most heterogenous malignancies characterized by equally heterogenous clinical outcomes. Standard morphological assessment with pathology and added immunohistochemical analyses is unable to fully address the heterogeneity, but up to now treatment decisions have been made based on such information only. Bladder cancer molecular subtypes will likely provide means for a more personalized bladder cancer care. Methods: To facilitate further development of bladder cancer molecular subtypes and clinical translation, the UROSCAN-biobank was initiated in 2013 to achieve systematic biobanking of preoperative blood and fresh frozen tumor tissue in a population-based setting. In a second phase, we... (More)

Background: Bladder cancer is molecularly one of the most heterogenous malignancies characterized by equally heterogenous clinical outcomes. Standard morphological assessment with pathology and added immunohistochemical analyses is unable to fully address the heterogeneity, but up to now treatment decisions have been made based on such information only. Bladder cancer molecular subtypes will likely provide means for a more personalized bladder cancer care. Methods: To facilitate further development of bladder cancer molecular subtypes and clinical translation, the UROSCAN-biobank was initiated in 2013 to achieve systematic biobanking of preoperative blood and fresh frozen tumor tissue in a population-based setting. In a second phase, we established in 2018 a parallel logistic pipeline for molecular profiling by RNA-sequencing, to develop and validate clinical implementation of molecular subtyping and actionable molecular target identification in real-time. Results: Until June 2021, 1825 individuals were included in the UROSCAN-biobank, of which 1650 (90%) had primary bladder cancer, 127 (7%) recurrent tumors, and 48 (3%) unknown tumor status. In 159 patients, multiple tumors were sampled, and metachronous tumors were collected in 83 patients. Between 2016 and 2020 the UROSCAN-biobanking included 1122/2999 (37%) of all primary bladder cancer patients in the Southern Healthcare Region. Until June 2021, the corresponding numbers subjected to RNA-sequencing and molecular subtyping was 605 (UROSCANSEQ), of which 52 (9%) samples were not sequenced due to inadequate RNA-quality (n = 47) or technical failure/lost sample (n = 5). Conclusions: The UROSCAN-biobanking and UROSCANSEQ-infrastructure for molecular subtyping by real-time RNA-sequencing represents, to our knowledge, the largest effort of evaluating population-wide molecular classification of bladder cancer.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
biobank, Bladder cancer, molecular subtyping, real-time, RNA-sequencing
in
Scandinavian Journal of Urology
volume
57
issue
1-6
pages
2 - 9
publisher
Taylor & Francis
external identifiers
  • pmid:36540001
  • scopus:85144550641
ISSN
2168-1805
DOI
10.1080/21681805.2022.2159519
language
English
LU publication?
yes
id
1c520a44-fb8a-4e35-8d6c-e369194ac9c8
date added to LUP
2023-01-23 12:48:06
date last changed
2024-03-19 13:42:57
@article{1c520a44-fb8a-4e35-8d6c-e369194ac9c8,
  abstract     = {{<p>Background: Bladder cancer is molecularly one of the most heterogenous malignancies characterized by equally heterogenous clinical outcomes. Standard morphological assessment with pathology and added immunohistochemical analyses is unable to fully address the heterogeneity, but up to now treatment decisions have been made based on such information only. Bladder cancer molecular subtypes will likely provide means for a more personalized bladder cancer care. Methods: To facilitate further development of bladder cancer molecular subtypes and clinical translation, the UROSCAN-biobank was initiated in 2013 to achieve systematic biobanking of preoperative blood and fresh frozen tumor tissue in a population-based setting. In a second phase, we established in 2018 a parallel logistic pipeline for molecular profiling by RNA-sequencing, to develop and validate clinical implementation of molecular subtyping and actionable molecular target identification in real-time. Results: Until June 2021, 1825 individuals were included in the UROSCAN-biobank, of which 1650 (90%) had primary bladder cancer, 127 (7%) recurrent tumors, and 48 (3%) unknown tumor status. In 159 patients, multiple tumors were sampled, and metachronous tumors were collected in 83 patients. Between 2016 and 2020 the UROSCAN-biobanking included 1122/2999 (37%) of all primary bladder cancer patients in the Southern Healthcare Region. Until June 2021, the corresponding numbers subjected to RNA-sequencing and molecular subtyping was 605 (UROSCANSEQ), of which 52 (9%) samples were not sequenced due to inadequate RNA-quality (n = 47) or technical failure/lost sample (n = 5). Conclusions: The UROSCAN-biobanking and UROSCANSEQ-infrastructure for molecular subtyping by real-time RNA-sequencing represents, to our knowledge, the largest effort of evaluating population-wide molecular classification of bladder cancer.</p>}},
  author       = {{Liedberg, Fredrik and Abrahamsson, Johan and Bernardo, Carina and Bläckberg, Mats and Edsjö, Anders and Heidenblad, Markus and Larsson, Christer and Sjödahl, Gottfrid and Eriksson, Pontus}},
  issn         = {{2168-1805}},
  keywords     = {{biobank; Bladder cancer; molecular subtyping; real-time; RNA-sequencing}},
  language     = {{eng}},
  number       = {{1-6}},
  pages        = {{2--9}},
  publisher    = {{Taylor & Francis}},
  series       = {{Scandinavian Journal of Urology}},
  title        = {{UROSCAN and UROSCANSEQ : a large-scale multicenter effort towards translation of molecular bladder cancer subtypes into clinical practice–from biobank to RNA-sequencing in real time}},
  url          = {{http://dx.doi.org/10.1080/21681805.2022.2159519}},
  doi          = {{10.1080/21681805.2022.2159519}},
  volume       = {{57}},
  year         = {{2023}},
}