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An alternative method for calibration of flow field flow fractionation channels for hydrodynamic radius determination : The nanoemulsion method (featuring multi angle light scattering)

Bolinsson, Hans LU ; Lu, Yi LU ; Hall, Stephen LU ; Nilsson, Lars LU and Håkansson, Andreas LU (2018) In Journal of Chromatography A 1533. p.155-163
Abstract

This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations. The novel method uses an oil-in-water nanoemulsion together with multi angle light scattering (MALS) and elution theory to determine channel height from an AF4 experiment. The method is validated using two orthogonal methods; first, by using standard particle elution experiments and, secondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy as with the traditional channel height... (More)

This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations. The novel method uses an oil-in-water nanoemulsion together with multi angle light scattering (MALS) and elution theory to determine channel height from an AF4 experiment. The method is validated using two orthogonal methods; first, by using standard particle elution experiments and, secondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy as with the traditional channel height determination technique. However, the nanoemulsion method can be used under more challenging conditions than standard particles, as the nanoemulsion remains stable in a wider pH range than the previously used standard particles. Moreover, the novel method is also more cost effective.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
AF4, Channel height, Flow field-flow fractionation, Multi angle light scattering, Nanoemulsion, X-ray computed tomography
in
Journal of Chromatography A
volume
1533
pages
9 pages
publisher
Elsevier
external identifiers
  • scopus:85039782632
ISSN
0021-9673
DOI
10.1016/j.chroma.2017.12.026
language
English
LU publication?
yes
id
1cd53846-3684-4920-b3c0-e2ff734d8c85
date added to LUP
2018-01-09 10:23:07
date last changed
2018-10-03 10:29:37
@article{1cd53846-3684-4920-b3c0-e2ff734d8c85,
  abstract     = {<p>This study suggests a novel method for determination of the channel height in asymmetrical flow field-flow fractionation (AF4), which can be used for calibration of the channel for hydrodynamic radius determinations. The novel method uses an oil-in-water nanoemulsion together with multi angle light scattering (MALS) and elution theory to determine channel height from an AF4 experiment. The method is validated using two orthogonal methods; first, by using standard particle elution experiments and, secondly, by imaging an assembled and carrier liquid filled channel by x-ray computed tomography (XCT). It is concluded that the channel height can be determined with approximately the same accuracy as with the traditional channel height determination technique. However, the nanoemulsion method can be used under more challenging conditions than standard particles, as the nanoemulsion remains stable in a wider pH range than the previously used standard particles. Moreover, the novel method is also more cost effective.</p>},
  author       = {Bolinsson, Hans and Lu, Yi and Hall, Stephen and Nilsson, Lars and Håkansson, Andreas},
  issn         = {0021-9673},
  keyword      = {AF4,Channel height,Flow field-flow fractionation,Multi angle light scattering,Nanoemulsion,X-ray computed tomography},
  language     = {eng},
  month        = {01},
  pages        = {155--163},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {An alternative method for calibration of flow field flow fractionation channels for hydrodynamic radius determination : The nanoemulsion method (featuring multi angle light scattering)},
  url          = {http://dx.doi.org/10.1016/j.chroma.2017.12.026},
  volume       = {1533},
  year         = {2018},
}