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Specificity of the H-3-triolein assay for pancreatic lipase in blood plasma

Gewert, K; Gregory, PC; Olivecrona, G; Erlanson-Albertsson, Charlotte LU and Pierzynowski, Stefan LU (2005) In Clinical Chemistry and Laboratory Medicine 43(11). p.1211-1214
Abstract
The aim of this study was to investigate the specificity of the H-3-triolein assay and to investigate the recovery of highly purified pancreatic lipase and pancreatic lipase in the form of pure non-activated pig pancreatic juice. Blood plasma from pigs was analysed for pancreatic lipase activity using the H-3-triolein substrate assay, with a method specific for lipoprotein lipase and with a method specific for hepatic lipase. The recovery of pancreatic lipase from pancreatic juice was approximately 100%, while the recovery of highly purified pancreatic lipase in plasma or whole blood was found to be approximately 1%. Preparations of highly concentrated, purified lipoprotein lipase showed activity in the H-3-triolein assay designed for... (More)
The aim of this study was to investigate the specificity of the H-3-triolein assay and to investigate the recovery of highly purified pancreatic lipase and pancreatic lipase in the form of pure non-activated pig pancreatic juice. Blood plasma from pigs was analysed for pancreatic lipase activity using the H-3-triolein substrate assay, with a method specific for lipoprotein lipase and with a method specific for hepatic lipase. The recovery of pancreatic lipase from pancreatic juice was approximately 100%, while the recovery of highly purified pancreatic lipase in plasma or whole blood was found to be approximately 1%. Preparations of highly concentrated, purified lipoprotein lipase showed activity in the H-3-triolein assay designed for pancreatic lipase, but the activity did not exceed 1% of the activity of this enzyme measured in an assay specific for lipoprotein lipase (samples containing physiological levels of lipoprotein lipase did not show any activity in the assay). Hepatic lipase was not measurable under the conditions of the H-3-triolein assay. In conclusion, the H-3-triolein assay showed pronounced specificity for pancreatic lipase compared with lipoprotein lipase or hepatic lipase. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hepatic lipase, lipoprotein lipase, pancreatic lipase
in
Clinical Chemistry and Laboratory Medicine
volume
43
issue
11
pages
1211 - 1214
publisher
De Gruyter
external identifiers
  • wos:000233747000007
  • pmid:16232087
  • scopus:27744458924
ISSN
1434-6621
DOI
10.1515/CCLM.2005.209
language
English
LU publication?
yes
id
47c1cad5-657d-4d9e-befd-788218061e8e (old id 211805)
date added to LUP
2007-08-24 13:23:50
date last changed
2017-01-01 05:06:05
@article{47c1cad5-657d-4d9e-befd-788218061e8e,
  abstract     = {The aim of this study was to investigate the specificity of the H-3-triolein assay and to investigate the recovery of highly purified pancreatic lipase and pancreatic lipase in the form of pure non-activated pig pancreatic juice. Blood plasma from pigs was analysed for pancreatic lipase activity using the H-3-triolein substrate assay, with a method specific for lipoprotein lipase and with a method specific for hepatic lipase. The recovery of pancreatic lipase from pancreatic juice was approximately 100%, while the recovery of highly purified pancreatic lipase in plasma or whole blood was found to be approximately 1%. Preparations of highly concentrated, purified lipoprotein lipase showed activity in the H-3-triolein assay designed for pancreatic lipase, but the activity did not exceed 1% of the activity of this enzyme measured in an assay specific for lipoprotein lipase (samples containing physiological levels of lipoprotein lipase did not show any activity in the assay). Hepatic lipase was not measurable under the conditions of the H-3-triolein assay. In conclusion, the H-3-triolein assay showed pronounced specificity for pancreatic lipase compared with lipoprotein lipase or hepatic lipase.},
  author       = {Gewert, K and Gregory, PC and Olivecrona, G and Erlanson-Albertsson, Charlotte and Pierzynowski, Stefan},
  issn         = {1434-6621},
  keyword      = {hepatic lipase,lipoprotein lipase,pancreatic lipase},
  language     = {eng},
  number       = {11},
  pages        = {1211--1214},
  publisher    = {De Gruyter},
  series       = {Clinical Chemistry and Laboratory Medicine},
  title        = {Specificity of the H-3-triolein assay for pancreatic lipase in blood plasma},
  url          = {http://dx.doi.org/10.1515/CCLM.2005.209},
  volume       = {43},
  year         = {2005},
}