Cognitive Impairment and Metabolite Profile Alterations in the Hippocampus and Cortex of Male and Female Mice Exposed to a Fat and Sugar-Rich Diet are Normalized by Diet Reversal
Garcia-Serrano, Alba LU ; Mohr, Adélaïde A ; Philippe, Juliette LU ; Skoug, Cecilia LU ; Spégel, Peter LU and Duarte, Joao NM LU
(2022)
In Aging and Disease
13(1).
p.267-283
- Abstract
- Diabetes impacts on brain metabolism, structure and function. Alterations in brain metabolism have been observed in obesity and diabetes models induced by exposure to diets rich in saturated fat and/or sugar and have been linked to memory impairment. However, it remains to be determined whether brain dysfunction induced by obesogenic diets results from permanent brain alterations. We tested the hypothesis that an obesogenic diet (high-fat and high-sucrose diet; HFHSD) causes reversible changes in hippocampus and cortex metabolism and alterations in behavior. Mice were exposed to HFHSD for 24 weeks or for 16 weeks followed by 8 weeks of diet normalization. Development of the metabolic syndrome, changes in behavior, and brain metabolite... (More)
- Diabetes impacts on brain metabolism, structure and function. Alterations in brain metabolism have been observed in obesity and diabetes models induced by exposure to diets rich in saturated fat and/or sugar and have been linked to memory impairment. However, it remains to be determined whether brain dysfunction induced by obesogenic diets results from permanent brain alterations. We tested the hypothesis that an obesogenic diet (high-fat and high-sucrose diet; HFHSD) causes reversible changes in hippocampus and cortex metabolism and alterations in behavior. Mice were exposed to HFHSD for 24 weeks or for 16 weeks followed by 8 weeks of diet normalization. Development of the metabolic syndrome, changes in behavior, and brain metabolite profiles by magnetic resonance spectroscopy (MRS) were assessed longitudinally. Control mice were fed an ingredient-matched low-fat and low-sugar diet. Mice fed the HFHSD developed obesity, glucose intolerance and insulin resistance, with a more severe phenotype in male than female mice. Relative to controls, both male and female HFHSD-fed mice showed increased anxiety-like behavior, impaired memory in object recognition tasks, but preserved working spatial memory as evaluated by spontaneous alternation in a Y-maze. Alterations in the metabolite profiles were observed both in the hippocampus and cortex but were more distinct in the hippocampus. HFHSD-induced metabolic changes included altered levels of lactate, glutamate, GABA, glutathione, taurine, N-acetylaspartate, total creatine and total choline. Notably, HFHSD-induced metabolic syndrome, anxiety, memory impairment, and brain metabolic alterations recovered upon diet normalization for 8 weeks. In conclusion, cortical and hippocampal derangements induced by long-term HFHSD consumption are reversible rather than being the result of permanent tissue damage. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/219fa0f9-4a3b-4ce0-8b1e-dab7fbfe5bc6
- author
- Garcia-Serrano, Alba
LU
; Mohr, Adélaïde A
; Philippe, Juliette
LU
; Skoug, Cecilia
LU
; Spégel, Peter
LU
and Duarte, Joao NM
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Aging and Disease
- volume
- 13
- issue
- 1
- pages
- 17 pages
- publisher
- Buck Institute for Age Research
- external identifiers
-
- scopus:85124414644
- pmid:35111373
- ISSN
- 2152-5250
- DOI
- 10.14336/AD.2021.0720
- language
- English
- LU publication?
- yes
- id
- 219fa0f9-4a3b-4ce0-8b1e-dab7fbfe5bc6
- date added to LUP
- 2021-09-21 20:27:07
- date last changed
- 2023-05-24 14:16:27
@article{219fa0f9-4a3b-4ce0-8b1e-dab7fbfe5bc6,
abstract = {{Diabetes impacts on brain metabolism, structure and function. Alterations in brain metabolism have been observed in obesity and diabetes models induced by exposure to diets rich in saturated fat and/or sugar and have been linked to memory impairment. However, it remains to be determined whether brain dysfunction induced by obesogenic diets results from permanent brain alterations. We tested the hypothesis that an obesogenic diet (high-fat and high-sucrose diet; HFHSD) causes reversible changes in hippocampus and cortex metabolism and alterations in behavior. Mice were exposed to HFHSD for 24 weeks or for 16 weeks followed by 8 weeks of diet normalization. Development of the metabolic syndrome, changes in behavior, and brain metabolite profiles by magnetic resonance spectroscopy (MRS) were assessed longitudinally. Control mice were fed an ingredient-matched low-fat and low-sugar diet. Mice fed the HFHSD developed obesity, glucose intolerance and insulin resistance, with a more severe phenotype in male than female mice. Relative to controls, both male and female HFHSD-fed mice showed increased anxiety-like behavior, impaired memory in object recognition tasks, but preserved working spatial memory as evaluated by spontaneous alternation in a Y-maze. Alterations in the metabolite profiles were observed both in the hippocampus and cortex but were more distinct in the hippocampus. HFHSD-induced metabolic changes included altered levels of lactate, glutamate, GABA, glutathione, taurine, N-acetylaspartate, total creatine and total choline. Notably, HFHSD-induced metabolic syndrome, anxiety, memory impairment, and brain metabolic alterations recovered upon diet normalization for 8 weeks. In conclusion, cortical and hippocampal derangements induced by long-term HFHSD consumption are reversible rather than being the result of permanent tissue damage.}},
author = {{Garcia-Serrano, Alba and Mohr, Adélaïde A and Philippe, Juliette and Skoug, Cecilia and Spégel, Peter and Duarte, Joao NM}},
issn = {{2152-5250}},
language = {{eng}},
number = {{1}},
pages = {{267--283}},
publisher = {{Buck Institute for Age Research}},
series = {{Aging and Disease}},
title = {{Cognitive Impairment and Metabolite Profile Alterations in the Hippocampus and Cortex of Male and Female Mice Exposed to a Fat and Sugar-Rich Diet are Normalized by Diet Reversal}},
url = {{http://dx.doi.org/10.14336/AD.2021.0720}},
doi = {{10.14336/AD.2021.0720}},
volume = {{13}},
year = {{2022}},
}