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Study on administration of 1,5-anhydro-D-fructose in C57BL/6J mice challenged with high-fat diet

Mei, Jie LU ; Yu, Shukun LU and Ahrén, Bo LU (2010) In BMC Endocrine Disorders 10.
Abstract
1,5-Anhydro-D-fructose (AF) is a mono-saccharide directly formed from starch and glycogen by the action of alpha-1,4-glucan lyase (EC 4.2.2.13). Our previous study has indicated that AF increases glucose tolerance and insulin secretion in NMRI mice after administration through a gastric gavage in a single dose at 150 mg per mouse. In this study, we used high-fat feeding of C57BL/6J mice to examine the influence of long-term administration of AF on glucose-stimulated insulin secretion in vivo and in vitro. We found that 8-weeks of high-fat feeding increased body weight, fasting blood glucose and insulin levels in C57BL/6J mice when compared to mice fed normal diet. Impaired glucose tolerance was also observed in mice receiving 8-weeks of... (More)
1,5-Anhydro-D-fructose (AF) is a mono-saccharide directly formed from starch and glycogen by the action of alpha-1,4-glucan lyase (EC 4.2.2.13). Our previous study has indicated that AF increases glucose tolerance and insulin secretion in NMRI mice after administration through a gastric gavage in a single dose at 150 mg per mouse. In this study, we used high-fat feeding of C57BL/6J mice to examine the influence of long-term administration of AF on glucose-stimulated insulin secretion in vivo and in vitro. We found that 8-weeks of high-fat feeding increased body weight, fasting blood glucose and insulin levels in C57BL/6J mice when compared to mice fed normal diet. Impaired glucose tolerance was also observed in mice receiving 8-weeks of high-fat diet. In contrast, AF (1.5 g/kg/day), administered through drinking water for 8-weeks, did not affect body weight or food and water intake in mice fed either the high-fat or normal diet. There was no difference in basal blood glucose or insulin levels between AF-treated and control group. Oral glucose tolerance test (OGTT) showed that AF did not affect glucose-stimulated insulin secretion in mice. In in vitro studies with isolated islets, AF did not influence glucose-stimulated insulin secretion in mice receiving either high-fat or normal diet. We therefore conclude that when given through drinking water for 8 weeks at 1.5 g/kg/day, AF has no effect on glucose-stimulated insulin secretion in C57BL/6J mice challenged with a high-fat diet. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BMC Endocrine Disorders
volume
10
publisher
BioMed Central
external identifiers
  • wos:000297287300001
  • scopus:77957902686
ISSN
1472-6823
DOI
10.1186/1472-6823-10-17
language
English
LU publication?
yes
id
6c8c5949-7a4a-4e86-8a60-896e43a9863a (old id 2227393)
date added to LUP
2011-12-23 12:27:24
date last changed
2018-05-29 09:48:39
@article{6c8c5949-7a4a-4e86-8a60-896e43a9863a,
  abstract     = {1,5-Anhydro-D-fructose (AF) is a mono-saccharide directly formed from starch and glycogen by the action of alpha-1,4-glucan lyase (EC 4.2.2.13). Our previous study has indicated that AF increases glucose tolerance and insulin secretion in NMRI mice after administration through a gastric gavage in a single dose at 150 mg per mouse. In this study, we used high-fat feeding of C57BL/6J mice to examine the influence of long-term administration of AF on glucose-stimulated insulin secretion in vivo and in vitro. We found that 8-weeks of high-fat feeding increased body weight, fasting blood glucose and insulin levels in C57BL/6J mice when compared to mice fed normal diet. Impaired glucose tolerance was also observed in mice receiving 8-weeks of high-fat diet. In contrast, AF (1.5 g/kg/day), administered through drinking water for 8-weeks, did not affect body weight or food and water intake in mice fed either the high-fat or normal diet. There was no difference in basal blood glucose or insulin levels between AF-treated and control group. Oral glucose tolerance test (OGTT) showed that AF did not affect glucose-stimulated insulin secretion in mice. In in vitro studies with isolated islets, AF did not influence glucose-stimulated insulin secretion in mice receiving either high-fat or normal diet. We therefore conclude that when given through drinking water for 8 weeks at 1.5 g/kg/day, AF has no effect on glucose-stimulated insulin secretion in C57BL/6J mice challenged with a high-fat diet.},
  author       = {Mei, Jie and Yu, Shukun and Ahrén, Bo},
  issn         = {1472-6823},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Endocrine Disorders},
  title        = {Study on administration of 1,5-anhydro-D-fructose in C57BL/6J mice challenged with high-fat diet},
  url          = {http://dx.doi.org/10.1186/1472-6823-10-17},
  volume       = {10},
  year         = {2010},
}