Study on administration of 1,5-anhydro-D-fructose in C57BL/6J mice challenged with high-fat diet
(2010) In BMC Endocrine Disorders 10.- Abstract
- 1,5-Anhydro-D-fructose (AF) is a mono-saccharide directly formed from starch and glycogen by the action of alpha-1,4-glucan lyase (EC 4.2.2.13). Our previous study has indicated that AF increases glucose tolerance and insulin secretion in NMRI mice after administration through a gastric gavage in a single dose at 150 mg per mouse. In this study, we used high-fat feeding of C57BL/6J mice to examine the influence of long-term administration of AF on glucose-stimulated insulin secretion in vivo and in vitro. We found that 8-weeks of high-fat feeding increased body weight, fasting blood glucose and insulin levels in C57BL/6J mice when compared to mice fed normal diet. Impaired glucose tolerance was also observed in mice receiving 8-weeks of... (More)
- 1,5-Anhydro-D-fructose (AF) is a mono-saccharide directly formed from starch and glycogen by the action of alpha-1,4-glucan lyase (EC 4.2.2.13). Our previous study has indicated that AF increases glucose tolerance and insulin secretion in NMRI mice after administration through a gastric gavage in a single dose at 150 mg per mouse. In this study, we used high-fat feeding of C57BL/6J mice to examine the influence of long-term administration of AF on glucose-stimulated insulin secretion in vivo and in vitro. We found that 8-weeks of high-fat feeding increased body weight, fasting blood glucose and insulin levels in C57BL/6J mice when compared to mice fed normal diet. Impaired glucose tolerance was also observed in mice receiving 8-weeks of high-fat diet. In contrast, AF (1.5 g/kg/day), administered through drinking water for 8-weeks, did not affect body weight or food and water intake in mice fed either the high-fat or normal diet. There was no difference in basal blood glucose or insulin levels between AF-treated and control group. Oral glucose tolerance test (OGTT) showed that AF did not affect glucose-stimulated insulin secretion in mice. In in vitro studies with isolated islets, AF did not influence glucose-stimulated insulin secretion in mice receiving either high-fat or normal diet. We therefore conclude that when given through drinking water for 8 weeks at 1.5 g/kg/day, AF has no effect on glucose-stimulated insulin secretion in C57BL/6J mice challenged with a high-fat diet. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2227393
- author
- Mei, Jie LU ; Yu, Shukun LU and Ahrén, Bo LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BMC Endocrine Disorders
- volume
- 10
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000297287300001
- scopus:77957902686
- pmid:20958989
- ISSN
- 1472-6823
- DOI
- 10.1186/1472-6823-10-17
- language
- English
- LU publication?
- yes
- id
- 6c8c5949-7a4a-4e86-8a60-896e43a9863a (old id 2227393)
- date added to LUP
- 2016-04-01 12:58:12
- date last changed
- 2024-11-22 00:10:28
@article{6c8c5949-7a4a-4e86-8a60-896e43a9863a,
abstract = {{1,5-Anhydro-D-fructose (AF) is a mono-saccharide directly formed from starch and glycogen by the action of alpha-1,4-glucan lyase (EC 4.2.2.13). Our previous study has indicated that AF increases glucose tolerance and insulin secretion in NMRI mice after administration through a gastric gavage in a single dose at 150 mg per mouse. In this study, we used high-fat feeding of C57BL/6J mice to examine the influence of long-term administration of AF on glucose-stimulated insulin secretion in vivo and in vitro. We found that 8-weeks of high-fat feeding increased body weight, fasting blood glucose and insulin levels in C57BL/6J mice when compared to mice fed normal diet. Impaired glucose tolerance was also observed in mice receiving 8-weeks of high-fat diet. In contrast, AF (1.5 g/kg/day), administered through drinking water for 8-weeks, did not affect body weight or food and water intake in mice fed either the high-fat or normal diet. There was no difference in basal blood glucose or insulin levels between AF-treated and control group. Oral glucose tolerance test (OGTT) showed that AF did not affect glucose-stimulated insulin secretion in mice. In in vitro studies with isolated islets, AF did not influence glucose-stimulated insulin secretion in mice receiving either high-fat or normal diet. We therefore conclude that when given through drinking water for 8 weeks at 1.5 g/kg/day, AF has no effect on glucose-stimulated insulin secretion in C57BL/6J mice challenged with a high-fat diet.}},
author = {{Mei, Jie and Yu, Shukun and Ahrén, Bo}},
issn = {{1472-6823}},
language = {{eng}},
publisher = {{BioMed Central (BMC)}},
series = {{BMC Endocrine Disorders}},
title = {{Study on administration of 1,5-anhydro-D-fructose in C57BL/6J mice challenged with high-fat diet}},
url = {{https://lup.lub.lu.se/search/files/3077517/2343267.pdf}},
doi = {{10.1186/1472-6823-10-17}},
volume = {{10}},
year = {{2010}},
}