Tumor selectivity at short times following systemic administration of a liposomal temoporfin formulation in a murine tumor model
(2007) In Photochemistry and Photobiology 83(5). p.1211-1219- Abstract
- Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is one of the most potent photodynamically active substances in clinical use. Treatment protocols for Temoporfin-mediated photodynamic therapy often rely on drug-light intervals of several days in order for the photosensitizer to accumulate within the target tissue, though tumor selectivity is limited. Here, the mTHPC localization was studied at 2-8 h following systemic administration of a liposomal Temoporfin formulation (0.15 mg kg(-1) b.w.) in HT29 human colon adermcarcinoma in NMRI nu/nu mice. Photosensitizer distribution within tumor and internal organs was investigated by means of high performance liquid chromatography following chemical extraction, as well as in situ... (More)
- Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is one of the most potent photodynamically active substances in clinical use. Treatment protocols for Temoporfin-mediated photodynamic therapy often rely on drug-light intervals of several days in order for the photosensitizer to accumulate within the target tissue, though tumor selectivity is limited. Here, the mTHPC localization was studied at 2-8 h following systemic administration of a liposomal Temoporfin formulation (0.15 mg kg(-1) b.w.) in HT29 human colon adermcarcinoma in NMRI nu/nu mice. Photosensitizer distribution within tumor and internal organs was investigated by means of high performance liquid chromatography following chemical extraction, as well as in situ fluorescence imaging and point-monitoring fluorescence spectroscopy. For tumor tissue, the Temoporfin concentrations at 4 h (0.16 +/- 0.024 ng mg(-1)) and 8 h (0.18 +/- 0.064 ng mg(-1)) were significantly higher than at 2 It (0.08 +/- 0.026 ng mg(-1)). The average tumor-to-muscle and the tumor-to-skin selectivity were 6.6 and 2, respectively, and did not vary significantly with time after photosensitizer injection. In plasma, the Temoporfin concentration was low (0.07 +/- 0.07 ng mg(-1)) and showed no significant variation with time. Our results indicate a rapid biodistribution and clearance from the bloodstream. Within the same type of organ, data from both fluorescence methods generally exhibited a significant correlation with the extraction results. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2259586
- author
- Svensson, Jenny LU ; Johansson, Ann LU ; Grafe, S ; Gitter, B ; Trebst, T ; Bendsoe, N ; Andersson-Engels, Stefan LU and Svanberg, Katarina LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Photochemistry and Photobiology
- volume
- 83
- issue
- 5
- pages
- 1211 - 1219
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000249881400027
- scopus:34548733709
- ISSN
- 0031-8655
- DOI
- 10.1111/j.1751-1097.2007.00146.x.
- language
- English
- LU publication?
- yes
- id
- 765cb90d-1629-466a-91d3-36d6799eb4fa (old id 2259586)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17880517&dopt=Abstract
- date added to LUP
- 2016-04-04 07:56:50
- date last changed
- 2025-01-05 22:49:34
@article{765cb90d-1629-466a-91d3-36d6799eb4fa,
abstract = {{Meso-tetra(hydroxyphenyl)chlorin (mTHPC) (INN: Temoporfin) is one of the most potent photodynamically active substances in clinical use. Treatment protocols for Temoporfin-mediated photodynamic therapy often rely on drug-light intervals of several days in order for the photosensitizer to accumulate within the target tissue, though tumor selectivity is limited. Here, the mTHPC localization was studied at 2-8 h following systemic administration of a liposomal Temoporfin formulation (0.15 mg kg(-1) b.w.) in HT29 human colon adermcarcinoma in NMRI nu/nu mice. Photosensitizer distribution within tumor and internal organs was investigated by means of high performance liquid chromatography following chemical extraction, as well as in situ fluorescence imaging and point-monitoring fluorescence spectroscopy. For tumor tissue, the Temoporfin concentrations at 4 h (0.16 +/- 0.024 ng mg(-1)) and 8 h (0.18 +/- 0.064 ng mg(-1)) were significantly higher than at 2 It (0.08 +/- 0.026 ng mg(-1)). The average tumor-to-muscle and the tumor-to-skin selectivity were 6.6 and 2, respectively, and did not vary significantly with time after photosensitizer injection. In plasma, the Temoporfin concentration was low (0.07 +/- 0.07 ng mg(-1)) and showed no significant variation with time. Our results indicate a rapid biodistribution and clearance from the bloodstream. Within the same type of organ, data from both fluorescence methods generally exhibited a significant correlation with the extraction results.}},
author = {{Svensson, Jenny and Johansson, Ann and Grafe, S and Gitter, B and Trebst, T and Bendsoe, N and Andersson-Engels, Stefan and Svanberg, Katarina}},
issn = {{0031-8655}},
language = {{eng}},
number = {{5}},
pages = {{1211--1219}},
publisher = {{Wiley-Blackwell}},
series = {{Photochemistry and Photobiology}},
title = {{Tumor selectivity at short times following systemic administration of a liposomal temoporfin formulation in a murine tumor model}},
url = {{https://lup.lub.lu.se/search/files/5160165/2297644.pdf}},
doi = {{10.1111/j.1751-1097.2007.00146.x.}},
volume = {{83}},
year = {{2007}},
}