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Gastrointestinal conditions influence the solution behaviour of cereal beta-glucans in vitro

Ulmius, Matilda LU ; Adapa, Srimannarayana; Önning, Gunilla LU and Nilsson, Lars LU (2012) In Food Chemistry 130(3). p.536-540
Abstract
The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific

detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans

in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed

... (More)
The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific

detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans

in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed

aggregates. Additionally, the aggregates from pure b-glucans were considerably denser after intestinal digestion. This may be construed as gel-formation in the small intestine, which should be tested for its relevance to health effects. Our results signal the difficulties in predicting b-glucan activity in the gastrointestinal tract purely from analysis of the fibre-rich product. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cereal b-glucan, Oat bran, Field-flow fractionation, Gastrointestinal model, Calcofluor
in
Food Chemistry
volume
130
issue
3
pages
536 - 540
publisher
Elsevier
external identifiers
  • wos:000296039400009
  • scopus:80052698795
ISSN
1873-7072
DOI
10.1016/j.foodchem.2011.07.066
language
English
LU publication?
yes
id
a8a94d8a-8da7-4a86-8f6a-22c4a7acc432 (old id 2277726)
date added to LUP
2012-01-09 09:16:16
date last changed
2017-09-24 03:09:19
@article{a8a94d8a-8da7-4a86-8f6a-22c4a7acc432,
  abstract     = {The solution behaviour of b-glucans in a gastrointestinal model was investigated in order to explore the mechanisms explaining the physiological effects of the soluble fibre. Asymmetrical flow field-flow fractionation was used to determine the molar mass distribution, and in-line calcofluor labelling allowed specific<br/><br>
detection of b-glucans in complex samples. When dispersed in water, weight-average molar mass (Mw) was determined to 1 * 10^6 g/mol for pure oat and barley b-glucans, and 200 * 10^6 g/mol for b-glucans<br/><br>
in oat bran, indicating that the b-glucans were aggregating. Samples from the gastric digestion displayed disrupted aggregates, while samples from the small intestinal digestion contained re-formed<br/><br>
aggregates. Additionally, the aggregates from pure b-glucans were considerably denser after intestinal digestion. This may be construed as gel-formation in the small intestine, which should be tested for its relevance to health effects. Our results signal the difficulties in predicting b-glucan activity in the gastrointestinal tract purely from analysis of the fibre-rich product.},
  author       = {Ulmius, Matilda and Adapa, Srimannarayana and Önning, Gunilla and Nilsson, Lars},
  issn         = {1873-7072},
  keyword      = {Cereal b-glucan,Oat bran,Field-flow fractionation,Gastrointestinal model,Calcofluor},
  language     = {eng},
  number       = {3},
  pages        = {536--540},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Gastrointestinal conditions influence the solution behaviour of cereal beta-glucans in vitro},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2011.07.066},
  volume       = {130},
  year         = {2012},
}