Advanced

Combined gene overexpression of neuropeptide Y and its receptor Y5 in the hippocampus suppresses seizures

Gotzsche, Casper R.; Nikitidou, Litsa LU ; Toft Sörensen, Andreas LU ; Olesen, Mikkel V.; Sorensen, Gunnar; Christiansen, Soren H. O.; Ängehagen, Mikael LU ; Woldbye, David P. D. and Kokaia, Merab LU (2012) In Neurobiology of Disease 45(1). p.288-296
Abstract
We recently demonstrated that recombinant adeno-associated viral vector-induced hippocampal overexpression of neuropeptide Y receptor, Y2, exerts a seizure-suppressant effect in kindling and kainate-induced models of epilepsy in rats. Interestingly, additional overexpression of neuropeptide Y in the hippocampus strengthened the seizure-suppressant effect of transgene Y2 receptors. Here we show for the first time that another neuropeptide Y receptor, Y5, can also be overexpressed in the hippocampus. However, unlike Y2 receptor overexpression, transgene Y5 receptors in the hippocampus had no effect on kainate-induced motor seizures in rats. However, combined overexpression of Y5 receptors and neuropeptide Y exerted prominent suppression of... (More)
We recently demonstrated that recombinant adeno-associated viral vector-induced hippocampal overexpression of neuropeptide Y receptor, Y2, exerts a seizure-suppressant effect in kindling and kainate-induced models of epilepsy in rats. Interestingly, additional overexpression of neuropeptide Y in the hippocampus strengthened the seizure-suppressant effect of transgene Y2 receptors. Here we show for the first time that another neuropeptide Y receptor, Y5, can also be overexpressed in the hippocampus. However, unlike Y2 receptor overexpression, transgene Y5 receptors in the hippocampus had no effect on kainate-induced motor seizures in rats. However, combined overexpression of Y5 receptors and neuropeptide Y exerted prominent suppression of seizures. This seizure-suppressant effect of combination gene therapy with Y5 receptors and neuropeptide Y was significantly stronger as compared to neuropeptide Y overexpression alone. These results suggest that overexpression of Y5 receptors in combination with neuropeptide Y could be an alternative approach for more effective suppression of hippocampal seizures. (C) 2011 Elsevier Inc. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Y5 neuropeptide Y receptor, Adeno-associated viral vectors, Kainic acid, seizures, Gene therapy
in
Neurobiology of Disease
volume
45
issue
1
pages
288 - 296
publisher
Elsevier
external identifiers
  • wos:000297883500032
  • scopus:81955162899
ISSN
0969-9961
DOI
10.1016/j.nbd.2011.08.012
language
English
LU publication?
yes
id
f046f8a3-49e9-4411-8a0e-52c410381986 (old id 2278874)
date added to LUP
2012-01-11 13:23:58
date last changed
2017-03-03 14:36:13
@article{f046f8a3-49e9-4411-8a0e-52c410381986,
  abstract     = {We recently demonstrated that recombinant adeno-associated viral vector-induced hippocampal overexpression of neuropeptide Y receptor, Y2, exerts a seizure-suppressant effect in kindling and kainate-induced models of epilepsy in rats. Interestingly, additional overexpression of neuropeptide Y in the hippocampus strengthened the seizure-suppressant effect of transgene Y2 receptors. Here we show for the first time that another neuropeptide Y receptor, Y5, can also be overexpressed in the hippocampus. However, unlike Y2 receptor overexpression, transgene Y5 receptors in the hippocampus had no effect on kainate-induced motor seizures in rats. However, combined overexpression of Y5 receptors and neuropeptide Y exerted prominent suppression of seizures. This seizure-suppressant effect of combination gene therapy with Y5 receptors and neuropeptide Y was significantly stronger as compared to neuropeptide Y overexpression alone. These results suggest that overexpression of Y5 receptors in combination with neuropeptide Y could be an alternative approach for more effective suppression of hippocampal seizures. (C) 2011 Elsevier Inc. All rights reserved.},
  author       = {Gotzsche, Casper R. and Nikitidou, Litsa and Toft Sörensen, Andreas and Olesen, Mikkel V. and Sorensen, Gunnar and Christiansen, Soren H. O. and Ängehagen, Mikael and Woldbye, David P. D. and Kokaia, Merab},
  issn         = {0969-9961},
  keyword      = {Y5 neuropeptide Y receptor,Adeno-associated viral vectors,Kainic acid,seizures,Gene therapy},
  language     = {eng},
  number       = {1},
  pages        = {288--296},
  publisher    = {Elsevier},
  series       = {Neurobiology of Disease},
  title        = {Combined gene overexpression of neuropeptide Y and its receptor Y5 in the hippocampus suppresses seizures},
  url          = {http://dx.doi.org/10.1016/j.nbd.2011.08.012},
  volume       = {45},
  year         = {2012},
}