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Characterization of the Lipid Droplet Proteome of a Clonal Insulin-producing β-Cell Line (INS-1 832/13).

Larsson, Sara LU ; Resjö, Svante LU ; Gomez, Maria LU ; James, Peter and Holm, Cecilia LU (2012) In Journal of Proteome Research 11(2). p.1264-1273
Abstract
Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins that were specific to droplets. The proteins fall into six categories based on function or previously observed localization: metabolism, endoplasmic reticulum/ribosomes, mitochondria, vesicle formation and transport, signaling, and miscellaneous.... (More)
Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins that were specific to droplets. The proteins fall into six categories based on function or previously observed localization: metabolism, endoplasmic reticulum/ribosomes, mitochondria, vesicle formation and transport, signaling, and miscellaneous. The protein profile reinforces the emerging picture of the lipid droplet as an active and dynamic organelle involved in lipid homeostasis and intracellular trafficking. Proteins belonging to the category mitochondria were highly represented, suggesting that the β-cell mitochondria and lipid droplets form a metabolic unit of potential relevance for insulin secretion. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
insulin, proteomics, lipid droplets, beta-cells
in
Journal of Proteome Research
volume
11
issue
2
pages
1264 - 1273
publisher
The American Chemical Society
external identifiers
  • wos:000300458300064
  • pmid:22268682
  • scopus:84856656225
ISSN
1535-3893
DOI
10.1021/pr200957p
language
English
LU publication?
yes
id
e3354c22-f260-44b1-acc8-24b207192dff (old id 2336060)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22268682?dopt=Abstract
date added to LUP
2012-02-01 22:46:20
date last changed
2017-10-01 03:29:52
@article{e3354c22-f260-44b1-acc8-24b207192dff,
  abstract     = {Lipids are known to play a crucial role both in the normal control of insulin release and in the deterioration of β-cell function, as observed in type 2 diabetes. Despite this established dual role of lipids, little is known about lipid storage and handling in β-cells. Here, we isolated lipid droplets from oleate-incubated INS-1 832/13 cells and characterized the lipid droplet proteome. In a total of four rounds of droplet isolation and proteomic analysis by HPLC-MS/MS, we identified 96 proteins that were specific to droplets. The proteins fall into six categories based on function or previously observed localization: metabolism, endoplasmic reticulum/ribosomes, mitochondria, vesicle formation and transport, signaling, and miscellaneous. The protein profile reinforces the emerging picture of the lipid droplet as an active and dynamic organelle involved in lipid homeostasis and intracellular trafficking. Proteins belonging to the category mitochondria were highly represented, suggesting that the β-cell mitochondria and lipid droplets form a metabolic unit of potential relevance for insulin secretion.},
  author       = {Larsson, Sara and Resjö, Svante and Gomez, Maria and James, Peter and Holm, Cecilia},
  issn         = {1535-3893},
  keyword      = {insulin,proteomics,lipid droplets,beta-cells},
  language     = {eng},
  number       = {2},
  pages        = {1264--1273},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Characterization of the Lipid Droplet Proteome of a Clonal Insulin-producing β-Cell Line (INS-1 832/13).},
  url          = {http://dx.doi.org/10.1021/pr200957p},
  volume       = {11},
  year         = {2012},
}