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Modeling of spectral changes for depth localization of fluorescent inclusion

Svensson, Jenny LU and Andersson-Engels, Stefan LU (2005) In Optics Express 13(11). p.4263-4274
Abstract
We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2-3 mm (relative error of 10-15%) in a 20 mm thick tissue slab.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Optics Express
volume
13
issue
11
pages
4263 - 4274
publisher
OSA
external identifiers
  • wos:000229534500043
  • scopus:21244469457
ISSN
1094-4087
language
English
LU publication?
yes
id
a0214f6d-85d6-4743-992e-a87481a38304 (old id 238674)
alternative location
http://www.opticsexpress.org/abstract.cfm?id=84168
date added to LUP
2007-08-20 09:46:43
date last changed
2017-01-01 07:13:14
@article{a0214f6d-85d6-4743-992e-a87481a38304,
  abstract     = {We have performed modeling of fluorescence signals from inclusions inside turbid media to investigate the influence of a limited fluorescence contrast and how accurately the depth can be determined by using the spectral information. The depth was determined by forming a ratio of simulated fluorescence intensities at two wavelengths. The results show that it is important to consider the background autofluorescence in determining the depth of a fluorescent inclusion. It is also necessary to know the optical properties of the tissue to obtain the depth. A 20% error in absorption or scattering coefficients yields an error in the determined depth of approximately 2-3 mm (relative error of 10-15%) in a 20 mm thick tissue slab.},
  author       = {Svensson, Jenny and Andersson-Engels, Stefan},
  issn         = {1094-4087},
  language     = {eng},
  number       = {11},
  pages        = {4263--4274},
  publisher    = {OSA},
  series       = {Optics Express},
  title        = {Modeling of spectral changes for depth localization of fluorescent inclusion},
  volume       = {13},
  year         = {2005},
}