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Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months

Ahren, Jonatan; Ahrén, Bo LU and Wierup, Nils LU (2010) In Islets 2(6). p.353-356
Abstract
As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this... (More)
As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta cell mass, insulin resistance, islet adaptation, apoptosis, proliferation
in
Islets
volume
2
issue
6
pages
353 - 356
publisher
Landes Bioscience
external identifiers
  • wos:000289689500003
  • scopus:78751563884
ISSN
1938-2022
DOI
10.4161/isl.2.6.13619
language
English
LU publication?
yes
id
24060c8a-6945-48a1-976d-8ea0e4e67362 (old id 1963975)
date added to LUP
2011-05-20 10:56:53
date last changed
2018-11-21 19:49:21
@article{24060c8a-6945-48a1-976d-8ea0e4e67362,
  abstract     = {As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with beta-cell expansion, we have now examined beta-cell volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta-cell volume was estimated as beta-cell area per islet, individual beta-cell size and beta-cell number per islet. Control animals were fed a normal chow (11% fat). We found that beta-cell area per islet and total number of beta-cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta-cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive beta-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive beta-cell expansion as evident by early increased islet beta-cell volume and total number of beta-cells, whereas individual beta-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta-cell volume.},
  author       = {Ahren, Jonatan and Ahrén, Bo and Wierup, Nils},
  issn         = {1938-2022},
  keyword      = {beta cell mass,insulin resistance,islet adaptation,apoptosis,proliferation},
  language     = {eng},
  number       = {6},
  pages        = {353--356},
  publisher    = {Landes Bioscience},
  series       = {Islets},
  title        = {Increased beta-cell volume in mice fed a high-fat diet A dynamic study over 12 months},
  url          = {http://dx.doi.org/10.4161/isl.2.6.13619},
  volume       = {2},
  year         = {2010},
}