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mRNA for pancreatic uncoupling protein 2 increases in two models of acute experimental pancreatitis in rats and mice

Segersvard, R; Rippe, Catarina LU ; DuPlantier, M; Herrington, MK; Isaksson, B; Adrian, TE; Erlanson-Albertsson, Charlotte LU and Permert, J (2005) In Cell and Tissue Research 320(2). p.251-258
Abstract
Uncoupling-protein 2 (UCP2) is a mitochondrial protein that appears to be involved in cellular oxidant defense and in the regulation of oncotic cell death, both of which are important features of acute pancreatitis. However, UCP2 expression in acute pancreatitis has not been previously reported. In the current experiments, pancreatic gene expression was studied by real-time reverse-transcription/polymerase chain reaction and Northern blots. Two models of acute experimental pancreatitis were investigated: cerulein-induced pancreatitis in mice at two different time points and taurocholate-induced pancreatitis in rats at two degrees of severity. After cerulein administration, acinar injury and leukocyte infiltration was significantly higher... (More)
Uncoupling-protein 2 (UCP2) is a mitochondrial protein that appears to be involved in cellular oxidant defense and in the regulation of oncotic cell death, both of which are important features of acute pancreatitis. However, UCP2 expression in acute pancreatitis has not been previously reported. In the current experiments, pancreatic gene expression was studied by real-time reverse-transcription/polymerase chain reaction and Northern blots. Two models of acute experimental pancreatitis were investigated: cerulein-induced pancreatitis in mice at two different time points and taurocholate-induced pancreatitis in rats at two degrees of severity. After cerulein administration, acinar injury and leukocyte infiltration was significantly higher at 24 h compared with 12 h after the first injection of cerulein (P < 0.05, P < 0.005, respectively). UCP2 mRNA was unchanged at 12 h but was nearly 12-fold greater than control levels after 24 h (P < 0.001). UCP2 gene expression correlated with acinar injury (r=0.69; P < 0.001). By 72 h after taurocholate administration, the severe group had more necrosis than the mild group (P < 0.005). Pancreatic UCP2 mRNA was increased fourfold in the severe group compared with controls (P < 0.01). UCP2 expression correlated with parenchymal necrosis (r=0.61; P < 0.01). Thus, pancreatic UCP2 mRNA increased in two models of acute pancreatitis. The increase in UCP2 gene expression was correlated with the severity of the disease. Up-regulation of UCP2 in the pancreas may be a protective response to oxidative stress, but this increase may also have a negative influence on cellular energy metabolism. Therefore, acinar UCP2 may be an important modifier of the severity of acute pancreatitis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
uncoupling proteins, acute pancreatitis, oxidative stress, rat (male Zucker), mouse, UCP2, (female C57/B16)
in
Cell and Tissue Research
volume
320
issue
2
pages
251 - 258
publisher
Springer
external identifiers
  • wos:000228728500006
  • pmid:15782323
  • scopus:18844388946
ISSN
1432-0878
DOI
10.1007/s00441-004-1024-1
language
English
LU publication?
yes
id
8a3e14e8-04e1-41f9-894d-d16091cf75a1 (old id 244514)
date added to LUP
2007-08-20 09:25:48
date last changed
2017-04-09 03:38:29
@article{8a3e14e8-04e1-41f9-894d-d16091cf75a1,
  abstract     = {Uncoupling-protein 2 (UCP2) is a mitochondrial protein that appears to be involved in cellular oxidant defense and in the regulation of oncotic cell death, both of which are important features of acute pancreatitis. However, UCP2 expression in acute pancreatitis has not been previously reported. In the current experiments, pancreatic gene expression was studied by real-time reverse-transcription/polymerase chain reaction and Northern blots. Two models of acute experimental pancreatitis were investigated: cerulein-induced pancreatitis in mice at two different time points and taurocholate-induced pancreatitis in rats at two degrees of severity. After cerulein administration, acinar injury and leukocyte infiltration was significantly higher at 24 h compared with 12 h after the first injection of cerulein (P &lt; 0.05, P &lt; 0.005, respectively). UCP2 mRNA was unchanged at 12 h but was nearly 12-fold greater than control levels after 24 h (P &lt; 0.001). UCP2 gene expression correlated with acinar injury (r=0.69; P &lt; 0.001). By 72 h after taurocholate administration, the severe group had more necrosis than the mild group (P &lt; 0.005). Pancreatic UCP2 mRNA was increased fourfold in the severe group compared with controls (P &lt; 0.01). UCP2 expression correlated with parenchymal necrosis (r=0.61; P &lt; 0.01). Thus, pancreatic UCP2 mRNA increased in two models of acute pancreatitis. The increase in UCP2 gene expression was correlated with the severity of the disease. Up-regulation of UCP2 in the pancreas may be a protective response to oxidative stress, but this increase may also have a negative influence on cellular energy metabolism. Therefore, acinar UCP2 may be an important modifier of the severity of acute pancreatitis.},
  author       = {Segersvard, R and Rippe, Catarina and DuPlantier, M and Herrington, MK and Isaksson, B and Adrian, TE and Erlanson-Albertsson, Charlotte and Permert, J},
  issn         = {1432-0878},
  keyword      = {uncoupling proteins,acute pancreatitis,oxidative stress,rat (male Zucker),mouse,UCP2,(female C57/B16)},
  language     = {eng},
  number       = {2},
  pages        = {251--258},
  publisher    = {Springer},
  series       = {Cell and Tissue Research},
  title        = {mRNA for pancreatic uncoupling protein 2 increases in two models of acute experimental pancreatitis in rats and mice},
  url          = {http://dx.doi.org/10.1007/s00441-004-1024-1},
  volume       = {320},
  year         = {2005},
}