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Oncostatin M signaling in human glioma cell lines

Krona, A ; Järnum, Sofia LU ; Salford, Leif LU ; Widegren, Bengt LU and Aman, P (2005) In Oncology Reports 13(5). p.807-811
Abstract
We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFR beta and OSMR beta. OSM treatment induced phosphorylation of STAT3 and STAT1... (More)
We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFR beta and OSMR beta. OSM treatment induced phosphorylation of STAT3 and STAT1 indicating presence of a functional JAK/STAT pathway. No OSM effect on proliferation was observed. OSM gave no protective effects against tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced cytotoxicity. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cytotoxicity, STAT3, proliferation, oncostatin M, astrocytoma, brain tumor
in
Oncology Reports
volume
13
issue
5
pages
807 - 811
publisher
D.A. Spandidos
external identifiers
  • pmid:15809742
  • wos:000228440500004
  • scopus:25144517324
ISSN
1791-2431
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Cell and Organism Biology (Closed 2011.) (011002100), Clinic of Neurosurgery: University Hospital, Lund (LUR000009), Department of Experimental Medical Science (013210000), Division IV (013230800)
id
e92327fb-85b9-4113-b55d-a22fe8b5da64 (old id 246339)
alternative location
http://147.52.72.117/OR/2005/volume13/number5/807.pdf
date added to LUP
2016-04-01 12:03:45
date last changed
2020-01-12 09:08:14
@article{e92327fb-85b9-4113-b55d-a22fe8b5da64,
  abstract     = {We have recently found that oncostatin M (OSM) is overexpressed in most human brain tumors. The effects of OSM are unclear with conflicting reports of growth stimulatory or inhibitory effects in various cell types. The aim of this study was to investigate the effects of OSM in 5 glioma cell lines and 7 short-term cultures of human gliomas and in normal cultured human astrocytes. None of the cell lines and short-term cultured tumor cells expressed OSM in vitro. OSM signals through a gp130 containing receptor complex over the JAK/STAT pathway. Immunofluorescence and RT-PCR analysis showed that the tumor cells express gp130 and the other receptor components, LIFR beta and OSMR beta. OSM treatment induced phosphorylation of STAT3 and STAT1 indicating presence of a functional JAK/STAT pathway. No OSM effect on proliferation was observed. OSM gave no protective effects against tumor necrosis factor-related apoptosis inducing ligand (TRAIL)-induced cytotoxicity.},
  author       = {Krona, A and Järnum, Sofia and Salford, Leif and Widegren, Bengt and Aman, P},
  issn         = {1791-2431},
  language     = {eng},
  number       = {5},
  pages        = {807--811},
  publisher    = {D.A. Spandidos},
  series       = {Oncology Reports},
  title        = {Oncostatin M signaling in human glioma cell lines},
  url          = {http://147.52.72.117/OR/2005/volume13/number5/807.pdf},
  volume       = {13},
  year         = {2005},
}