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Wholegrain oat diet changes the expression of genes associated with intestinal bile acid transport

Andersson, Kristina E. LU ; Chawade, Aakash ; Thuresson, Narda LU ; Rascon, Ana LU ; Öste, Rickard LU ; Sterner, Olov LU ; Olsson, Olof LU and Hellstrand, Per LU (2017) In Molecular Nutrition and Food Research 61(7).
Abstract

Scope: The molecular mechanisms underlying the cholesterol-lowering properties of oats are only partly known. To study possible pathways involved, we investigated gene expressions in the liver and small intestine of mice fed oats. Method and results: Cholesterol and bile acids were analyzed in plasma and feces from LDL-receptor deficient (LDLr-/-) mice fed Western diet with wholegrain oats. A transcriptome analysis of mRNA from liver and jejunum was performed together with quantitative RT-PCR. Oat-fed mice had lower levels of plasma lipids and increased levels of bile acids and cholesterol in feces compared with controls. Two hundred thirty nine genes in jejunum and 25 genes in liver were differentially expressed (FDR... (More)

Scope: The molecular mechanisms underlying the cholesterol-lowering properties of oats are only partly known. To study possible pathways involved, we investigated gene expressions in the liver and small intestine of mice fed oats. Method and results: Cholesterol and bile acids were analyzed in plasma and feces from LDL-receptor deficient (LDLr-/-) mice fed Western diet with wholegrain oats. A transcriptome analysis of mRNA from liver and jejunum was performed together with quantitative RT-PCR. Oat-fed mice had lower levels of plasma lipids and increased levels of bile acids and cholesterol in feces compared with controls. Two hundred thirty nine genes in jejunum and 25 genes in liver were differentially expressed (FDR corrected p < 0.05). The most affected biological process in jejunum was lipid biosynthesis and regulation. The apical sodium-dependent bile acid transporter (ASBT, Slc10a) and the intracellular bile acid binding protein (Fabp6) were both upregulated, whereas small heterodimer partner-1 (Shp-1) and apolipoprotein CII (Apoc2) were downregulated. Conclusions: Whole oats attenuated responses typically induced by high-fat diet. Increased expression of genes for intestinal bile acid uptake following oat consumption suggests retention in the gut lumen rather than decreased uptake capacity as cause for the increased bile acid excretion and the concomitant reduction of plasma cholesterol.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bile acids, Cholesterol, Diet, Gene expression, Oats
in
Molecular Nutrition and Food Research
volume
61
issue
7
article number
1600874
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:28205325
  • wos:000404730400010
  • scopus:85017033426
ISSN
1613-4125
DOI
10.1002/mnfr.201600874
language
English
LU publication?
yes
id
24978bd5-af0d-4252-8ede-45815aaaa166
date added to LUP
2017-05-05 13:23:32
date last changed
2025-01-07 12:36:12
@article{24978bd5-af0d-4252-8ede-45815aaaa166,
  abstract     = {{<p>Scope: The molecular mechanisms underlying the cholesterol-lowering properties of oats are only partly known. To study possible pathways involved, we investigated gene expressions in the liver and small intestine of mice fed oats. Method and results: Cholesterol and bile acids were analyzed in plasma and feces from LDL-receptor deficient (LDLr<sup>-/-</sup>) mice fed Western diet with wholegrain oats. A transcriptome analysis of mRNA from liver and jejunum was performed together with quantitative RT-PCR. Oat-fed mice had lower levels of plasma lipids and increased levels of bile acids and cholesterol in feces compared with controls. Two hundred thirty nine genes in jejunum and 25 genes in liver were differentially expressed (FDR corrected p &lt; 0.05). The most affected biological process in jejunum was lipid biosynthesis and regulation. The apical sodium-dependent bile acid transporter (ASBT, Slc10a) and the intracellular bile acid binding protein (Fabp6) were both upregulated, whereas small heterodimer partner-1 (Shp-1) and apolipoprotein CII (Apoc2) were downregulated. Conclusions: Whole oats attenuated responses typically induced by high-fat diet. Increased expression of genes for intestinal bile acid uptake following oat consumption suggests retention in the gut lumen rather than decreased uptake capacity as cause for the increased bile acid excretion and the concomitant reduction of plasma cholesterol.</p>}},
  author       = {{Andersson, Kristina E. and Chawade, Aakash and Thuresson, Narda and Rascon, Ana and Öste, Rickard and Sterner, Olov and Olsson, Olof and Hellstrand, Per}},
  issn         = {{1613-4125}},
  keywords     = {{Bile acids; Cholesterol; Diet; Gene expression; Oats}},
  language     = {{eng}},
  number       = {{7}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Molecular Nutrition and Food Research}},
  title        = {{Wholegrain oat diet changes the expression of genes associated with intestinal bile acid transport}},
  url          = {{http://dx.doi.org/10.1002/mnfr.201600874}},
  doi          = {{10.1002/mnfr.201600874}},
  volume       = {{61}},
  year         = {{2017}},
}