Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans

Sunds, Anne Vuholm ; Roland, Ida Schwartz ; Sundekilde, Ulrik Kræmer ; Thesbjerg, Martin Nørmark ; Robinson, Randall ; Bunyatratchata, Apichaya ; Glantz, Maria LU ; Paulsson, Marie LU ; Leskauskaite, Daiva and Pihlanto, Anne , et al. (2021) In Metabolites 11(10).
Abstract

Little is known about the extent of variation and activity of naturally occurring milk glycosidases and their potential to degrade milk glycans. A multi-omics approach was used to investigate the relationship between glycosidases and important bioactive compounds such as free oligo-saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays activities of eight indigenous glycosidases were determined, and by mass spectrometry and1H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk samples from eight native North European cattle breeds. The results showed a clear variation in glycosidase activities among the native breeds. Interestingly, negative... (More)

Little is known about the extent of variation and activity of naturally occurring milk glycosidases and their potential to degrade milk glycans. A multi-omics approach was used to investigate the relationship between glycosidases and important bioactive compounds such as free oligo-saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays activities of eight indigenous glycosidases were determined, and by mass spectrometry and1H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk samples from eight native North European cattle breeds. The results showed a clear variation in glycosidase activities among the native breeds. Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-D-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, P-value < 0.001) indicating cleavage of fucosylated glycans in milk at room temperature. The results obtained suggest that milk glycosidases might partially degrade valuable glycans, which would result in lower recovery of glycans and thus represent a loss for the dairy ingredients industry if these activities are pronounced.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cattle breeds, Mass spectrometry, Natural glycosidases, NMR spectroscopy, O-linked glycans, Oligosaccharides, Principal component analysis
in
Metabolites
volume
11
issue
10
article number
662
publisher
MDPI AG
external identifiers
  • pmid:34677377
  • scopus:85116125407
ISSN
2218-1989
DOI
10.3390/metabo11100662
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
id
26663814-b515-4df5-8772-955d58aa5a0b
date added to LUP
2021-10-20 15:38:20
date last changed
2024-06-15 18:31:47
@article{26663814-b515-4df5-8772-955d58aa5a0b,
  abstract     = {{<p>Little is known about the extent of variation and activity of naturally occurring milk glycosidases and their potential to degrade milk glycans. A multi-omics approach was used to investigate the relationship between glycosidases and important bioactive compounds such as free oligo-saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays activities of eight indigenous glycosidases were determined, and by mass spectrometry and<sup>1</sup>H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk samples from eight native North European cattle breeds. The results showed a clear variation in glycosidase activities among the native breeds. Interestingly, negative correlations between some glycosidases including β-galactosidase, N-acetyl-β-D-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, P-value &lt; 0.001) indicating cleavage of fucosylated glycans in milk at room temperature. The results obtained suggest that milk glycosidases might partially degrade valuable glycans, which would result in lower recovery of glycans and thus represent a loss for the dairy ingredients industry if these activities are pronounced.</p>}},
  author       = {{Sunds, Anne Vuholm and Roland, Ida Schwartz and Sundekilde, Ulrik Kræmer and Thesbjerg, Martin Nørmark and Robinson, Randall and Bunyatratchata, Apichaya and Glantz, Maria and Paulsson, Marie and Leskauskaite, Daiva and Pihlanto, Anne and Inglingstad, Ragnhild and Devold, Tove Gulbrandsen and Vegarud, Gerd Elisabeth and Birgisdottir, Bryndis Eva and Gudjonsdottir, Maria and Barile, Daniela and Larsen, Lotte Bach and Poulsen, Nina Aagaard}},
  issn         = {{2218-1989}},
  keywords     = {{Cattle breeds; Mass spectrometry; Natural glycosidases; NMR spectroscopy; O-linked glycans; Oligosaccharides; Principal component analysis}},
  language     = {{eng}},
  number       = {{10}},
  publisher    = {{MDPI AG}},
  series       = {{Metabolites}},
  title        = {{Naturally occurring glycosidases in milk from native cattle breeds : Activity and consequences on free and protein bound-glycans}},
  url          = {{http://dx.doi.org/10.3390/metabo11100662}},
  doi          = {{10.3390/metabo11100662}},
  volume       = {{11}},
  year         = {{2021}},
}