Identification and characterization of antigen-specific t-cells in viral infections
(2024) In Methods in Molecular Biology 2813. p.295-308- Abstract
Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2939ded2-87f9-4799-b238-2fca7a7f47fc
- author
- Trubach, Darya ; Muleta, Konjit Getachew LU ; Lahl, Katharina LU and Saini, Sunil Kumar
- organization
- publishing date
- 2024
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- CD8+ T-cells, Cytokine secretion assay, Flow cytometry, Rotavirus, SARS-CoV-2, T-cell epitopes, T-cell staining, Tetramers
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 2813
- pages
- 14 pages
- publisher
- Humana Press
- external identifiers
-
- scopus:85196598846
- pmid:38888785
- ISSN
- 1064-3745
- 1940-6029
- DOI
- 10.1007/978-1-0716-3890-3_19
- language
- English
- LU publication?
- yes
- additional info
- ยด
- id
- 2939ded2-87f9-4799-b238-2fca7a7f47fc
- date added to LUP
- 2024-09-04 15:07:00
- date last changed
- 2024-09-18 16:20:13
@inbook{2939ded2-87f9-4799-b238-2fca7a7f47fc, abstract = {{<p>Identification and characterization of CD8<sup>+</sup> T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantifica-tion and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.</p>}}, author = {{Trubach, Darya and Muleta, Konjit Getachew and Lahl, Katharina and Saini, Sunil Kumar}}, booktitle = {{Methods in Molecular Biology}}, issn = {{1064-3745}}, keywords = {{CD8+ T-cells; Cytokine secretion assay; Flow cytometry; Rotavirus; SARS-CoV-2; T-cell epitopes; T-cell staining; Tetramers}}, language = {{eng}}, pages = {{295--308}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Identification and characterization of antigen-specific t-cells in viral infections}}, url = {{http://dx.doi.org/10.1007/978-1-0716-3890-3_19}}, doi = {{10.1007/978-1-0716-3890-3_19}}, volume = {{2813}}, year = {{2024}}, }